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. 1989 Mar;63(3):1023–1030. doi: 10.1128/jvi.63.3.1023-1030.1989

Replication of adeno-associated virus in cells irradiated with UV light at 254 nm.

B Yakobson 1, T A Hrynko 1, M J Peak 1, E Winocour 1
PMCID: PMC247794  PMID: 2536816

Abstract

Irradiation of simian virus 40 (ori mutant)-transformed Chinese hamster embryo cells (OD4 line) with UV light induced a cellular capacity which supported a full cycle of helper-independent adeno-associated virus replication. Monochromatic UV light at 254 nm was about 1,000-fold more effective than UV light at 313 nm, indicating that cellular nucleic acid is the primary chromophore in the UV-induced process leading to permissiveness for adeno-associated virus replication. The UV irradiation and the infection could be separated for up to 12 h without substantial loss of permissiveness. During this time interval, the induction process was partly sensitive to cycloheximide, suggesting a requirement for de novo protein synthesis.

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Selected References

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