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. 1970 Jul;103(1):71–80. doi: 10.1128/jb.103.1.71-80.1970

Genetic Exchange Between Escherichia coli Strains in the Mouse Intestine

Randall T Jones 1,2,1, Roy Curtiss III 1,2
PMCID: PMC248040  PMID: 4912531

Abstract

Germ-free mice contaminated with selected Escherichia coli strains were used for experiments designed to demonstrate gene transfer and recombinant formation in vivo. The well-characterized conjugation system of E. coli K-12 was examined in these experiments. Contamination of germ-free mice with a polyauxotrophic F− strain followed by the addition of isogenic Hfr, F′, or F+ strains resulted in the appearance of all recombinant classes at frequencies that would be expected from an in vitro mating experiment. Inheritance of unselected donor markers occurred at frequencies that were dependent on linkage relationships established in experiments in vitro. The presence of Lactobacillus had no influence on gene transfer and recombinant formation in an F′ × F− in vivo mating. The R factor ROR-1 was transferred from E. coli strain M7-18 to an E. coli F− strain in the mouse intestine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berg C. M., Curtiss R., 3rd Transposition derivatives of an Hfr strain of Escherichia coli K-12. Genetics. 1967 Jul;56(3):503–525. doi: 10.1093/genetics/56.3.503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CURTIS S. R., 3rd CHROMOSOMAL ABERRATIONS ASSOCIATED WITH MUTATIONS TO BACTERIOPHAGE RESISTANCE IN ESCHERICHIA COLI. J Bacteriol. 1965 Jan;89:28–40. doi: 10.1128/jb.89.1.28-40.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Curtiss R., 3rd Bacterial conjugation. Annu Rev Microbiol. 1969;23:69–136. doi: 10.1146/annurev.mi.23.100169.000441. [DOI] [PubMed] [Google Scholar]
  4. Curtiss R., 3rd, Caro L. G., Allison D. P., Stallions D. R. Early stages of conjugation in Escherichia coli. J Bacteriol. 1969 Nov;100(2):1091–1104. doi: 10.1128/jb.100.2.1091-1104.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Curtiss R., 3rd, Charamella L. J., Stallions D. R., Mays J. A. Parental functions during conjugation in Escherichia coli K-12. Bacteriol Rev. 1968 Dec;32(4 Pt 1):320–348. [PMC free article] [PubMed] [Google Scholar]
  6. Curtiss R., 3rd, Stallions D. R. Probability of F integration and frequency of stable Hfr donors in F+ populations of Escherichia coli K-12. Genetics. 1969 Sep;63(1):27–38. doi: 10.1093/genetics/63.1.27. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Curtiss R., 3rd Ultraviolet-induced genetic recombination in a partially diploid strain of Escherichia coli. Genetics. 1968 Jan;58(1):9–54. doi: 10.1093/genetics/58.1.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. DAWSON G. W., SMITH-KEARY P. F. Episomic control of mutation in Salmonella typhimurium. Heredity (Edinb) 1963 Feb;18:1–20. doi: 10.1038/hdy.1963.1. [DOI] [PubMed] [Google Scholar]
  9. Ducluzeau R., Galinha A. Recombinaison in vivo entre une souche Hfr et une souche F d'Escherichia coli K 12, ensemencées dans le tube digestif de souris axéniques. C R Acad Sci Hebd Seances Acad Sci D. 1967 Jan 4;264(1):177–180. [PubMed] [Google Scholar]
  10. Ducluzeau R., Raibaud P. Sur la sélection in vivo des recombinants issus du croisement entre deux souches d'Escherichia coli ensemencées dans le tube digestif de souris axéniques. C R Acad Sci Hebd Seances Acad Sci D. 1968 May 6;266(19):2013–2016. [PubMed] [Google Scholar]
  11. FONG J., CHIN D., ELBERG S. S. Studies on tubercle bacillusmonocyte relationship. IV. Effects of passage in normal and immune systems upon virulent bacilli. J Exp Med. 1961 Jul 1;114:75–87. doi: 10.1084/jem.114.1.75. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Guinée P. A. Transfer of multiple drug resistance from Escherichia coli to Salmonella typhi murium in the mouse intestine. Antonie Van Leeuwenhoek. 1965;31(3):314–322. doi: 10.1007/BF02045911. [DOI] [PubMed] [Google Scholar]
  13. Jarolmen H., Kemp G. R factor transmission in vivo. J Bacteriol. 1969 Aug;99(2):487–490. doi: 10.1128/jb.99.2.487-490.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. KASUYA M. TRANSFER OF DRUG RESISTANCE BETWEEN ENTERIC BACTERIA INDUCED IN THE MOUSE INTESTINE. J Bacteriol. 1964 Aug;88:322–328. doi: 10.1128/jb.88.2.322-328.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. LEDERBERG J., LEDERBERG E. M. Replica plating and indirect selection of bacterial mutants. J Bacteriol. 1952 Mar;63(3):399–406. doi: 10.1128/jb.63.3.399-406.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. LEDERBERG J., LEDERBERG E. M., ZINDER N. D., LIVELY E. R. Recombination analysis of bacterial heredity. Cold Spring Harb Symp Quant Biol. 1951;16:413–443. doi: 10.1101/sqb.1951.016.01.030. [DOI] [PubMed] [Google Scholar]
  17. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  18. Lederberg J. Gene Recombination and Linked Segregations in Escherichia Coli. Genetics. 1947 Sep;32(5):505–525. doi: 10.1093/genetics/32.5.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Low B. Low recombination frequency for markers very near the origin in conjugation in E. coli. Genet Res. 1965 Nov;6(3):469–473. doi: 10.1017/s0016672300004341. [DOI] [PubMed] [Google Scholar]
  20. Meynell E., Meynell G. G., Datta N. Phylogenetic relationships of drug-resistance factors and other transmissible bacterial plasmids. Bacteriol Rev. 1968 Mar;32(1):55–83. doi: 10.1128/br.32.1.55-83.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. NOVICK R. P., RICHMOND M. H. NATURE AND INTERACTIONS OF THE GENETIC ELEMENTS GOVERNING PENICILLINASE SYNTHESIS IN STAPHYLOCOCCUS AUREUS. J Bacteriol. 1965 Aug;90:467–480. doi: 10.1128/jb.90.2.467-480.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Nomura M. Colicins and related bacteriocins. Annu Rev Microbiol. 1967;21:257–284. doi: 10.1146/annurev.mi.21.100167.001353. [DOI] [PubMed] [Google Scholar]
  23. Orskov I., Orskov F. Episome-carried surface antigen K88 of Escherichia coli. I. Transmission of the determinant of the K88 antigen and influence on the transfer of chromosomal markers. J Bacteriol. 1966 Jan;91(1):69–75. doi: 10.1128/jb.91.1.69-75.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Reed N. D., Sieckmann D. G., Georgi C. E. Transfer of infectious drug resistance in microbially defined mice. J Bacteriol. 1969 Oct;100(1):22–26. doi: 10.1128/jb.100.1.22-26.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. SUGINO Y., HIROTA Y. Conjugal fertility associated with resistance factor R in Escherichia coli. J Bacteriol. 1962 Nov;84:902–910. doi: 10.1128/jb.84.5.902-910.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Salzman T. C., Klemm L. Transfer of antibiotic resistance (R factor) in the mouse intestine. Proc Soc Exp Biol Med. 1968 Jun;128(2):392–394. doi: 10.3181/00379727-128-33020. [DOI] [PubMed] [Google Scholar]
  27. Smith H. W., Halls S. The transmissible nature of the genetic factor in Escherichia coli that controls haemolysin production. J Gen Microbiol. 1967 Apr;47(1):153–161. doi: 10.1099/00221287-47-1-153. [DOI] [PubMed] [Google Scholar]
  28. TREXLER P. C., REYNOLDS L. I. Flexible film apparatus for the rearing and use of germfree animals. Appl Microbiol. 1957 Nov;5(6):406–412. doi: 10.1128/am.5.6.406-412.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. WALBURG H. E., Jr, UPTON A. C., TYNDALL R. L., HARRIS W. W., COSGROVE G. E. PRELIMINARY OBSERVATIONS ON SPONTANEOUS AND RADIATION-INDUCED LEUKEMIA IN GERMFREE MICE. Proc Soc Exp Biol Med. 1965 Jan;118:11–14. doi: 10.3181/00379727-118-29743. [DOI] [PubMed] [Google Scholar]
  30. WATANABE T. Infective heredity of multiple drug resistance in bacteria. Bacteriol Rev. 1963 Mar;27:87–115. doi: 10.1128/br.27.1.87-115.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Walton J. In vivo transfer of infectious drug resistance. Nature. 1966 Jul 16;211(5046):312–313. doi: 10.1038/211312a0. [DOI] [PubMed] [Google Scholar]

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