Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1970 Oct;104(1):434–442. doi: 10.1128/jb.104.1.434-442.1970

Polyphasic Taxonomy of the Genus Vibrio: Polynucleotide Sequence Relationships Among Selected Vibrio Species

R V Citarella 1, R R Colwell 1
PMCID: PMC248228  PMID: 5473902

Abstract

Polynucleotide relationships among selected Vibrio species were examined by means of deoxyribonucleic acid (DNA) reassociation reactions and chromatography on hydroxyapatite. Relative levels of intraspecific DNA duplex formation (V. cholerae-V. cholerae and V. parahaemolyticus-V. parahaemolyticus) were found to be high at 60 C (>80%), and only minimally reduced at 75 C. Interspecific DNA duplexes between V. cholerae DNA and that of the non-cholera vibrios also exhibited high relative levels of formation at 60 C (>80%) and, with one exception, were only slightly reduced at 75 C. The thermal stability of these duplexes formed at 60 or 75 C was virtually identical to that of homologous V. cholerae DNA duplexes. The degree of reassociation and the thermal stability of V. cholerae-non-cholera vibrio DNA duplexes suggests relatively little evolutionary divergence in these organisms. In all other interspecific DNA reassociation reactions, only low levels of DNA duplex formation were noted at 60 C (<25%), and these were drastically reduced (>50%) at 75 C. The degree of nucleotide sequence divergence indicated by these reactions suggests that these Vibrio species are not significantly related to V. cholerae or V. parahaemolyticus. Reassociation reactions between V. cholerae DNA and the DNA of V. parahaemolyticus indicated these species were not significantly related to each other.

Full text

PDF
442

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brenner D. J., Fanning G. R., Johnson K. E., Citarella R. V., Falkow S. Polynucleotide sequence relationships among members of Enterobacteriaceae. J Bacteriol. 1969 May;98(2):637–650. doi: 10.1128/jb.98.2.637-650.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brenner D. J., Fanning G. R., Rake A. V., Johnson K. E. Batch procedure for thermal elution of DNA from hydroxyapatite. Anal Biochem. 1969 Apr 4;28(1):447–459. doi: 10.1016/0003-2697(69)90199-7. [DOI] [PubMed] [Google Scholar]
  3. Britten R. J., Kohne D. E. Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms. Science. 1968 Aug 9;161(3841):529–540. doi: 10.1126/science.161.3841.529. [DOI] [PubMed] [Google Scholar]
  4. COLWELL R. R., MANDEL M. ADANSONIAN ANALYSIS AND DEOXYRIBONUCLEIC ACID BASE COMPOSITION OF SOME GRAM-NEGATIVE BACTERIA. J Bacteriol. 1964 Jun;87:1412–1422. doi: 10.1128/jb.87.6.1412-1422.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. COLWELL R. R., MORITA R. Y. REISOLATION AND EMENDATION OF DESCRIPTION OF VIBRIO MARINUS (RUSSELL) FORD. J Bacteriol. 1964 Oct;88:831–837. doi: 10.1128/jb.88.4.831-837.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Clausen T. Measurement of 32P activity in a liquid scintillation counter without the use of scintillator. Anal Biochem. 1968 Jan;22(1):70–73. doi: 10.1016/0003-2697(68)90260-1. [DOI] [PubMed] [Google Scholar]
  7. Colwell R. R. Polyphasic taxonomy of the genus vibrio: numerical taxonomy of Vibrio cholerae, Vibrio parahaemolyticus, and related Vibrio species. J Bacteriol. 1970 Oct;104(1):410–433. doi: 10.1128/jb.104.1.410-433.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. DAVIS G. H., PARK R. W. A taxonomic study of certain bacteria currently classified as Vibrio species. J Gen Microbiol. 1962 Jan;27:101–119. doi: 10.1099/00221287-27-1-101. [DOI] [PubMed] [Google Scholar]
  9. Felsenfeld O. A review of recent trends in cholera research and control. With an annex on the isolation and identification of cholera vibrios. Bull World Health Organ. 1966;34(2):161–195. [PMC free article] [PubMed] [Google Scholar]
  10. Fujino T., Miwatani T., Yasuda J., Kondo M., Takeda Y., Akita Y., Kotera K., Okada M., Nishimune H., Shimizu Y. Taxonomic studies on the bacterial strains isolated from cases of "shirasu" food-poisoning (Pasteurella parahaemolytica) and related microorganisms. Biken J. 1965 Jun;8(2):63–71. [PubMed] [Google Scholar]
  11. HANSEN A. J., WEEKS O. B., COLWELL R. R. TAXONOMY OF PSEUDOMONAS PISCICIDA (BEIN) BUCK, MEYERS, AND LEIFSON. J Bacteriol. 1965 Mar;89:752–761. doi: 10.1128/jb.89.3.752-761.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hanaoka M., Kato Y., Amano T. Complementary examination of DNA'S among Vibrio species. Biken J. 1969 Sep;12(3):181–185. [PubMed] [Google Scholar]
  13. Hill L. R. An index to deoxyribonucleic acid base compositions of bacterial species. J Gen Microbiol. 1966 Sep;44(3):419–437. doi: 10.1099/00221287-44-3-419. [DOI] [PubMed] [Google Scholar]
  14. Krantz G. E., Colwell R. R., Lovelace E. Vibrio parahaemolyticus from the blue crab Callinectes sapidus in Chesapeake Bay. Science. 1969 Jun 13;164(3885):1286–1287. doi: 10.1126/science.164.3885.1286. [DOI] [PubMed] [Google Scholar]
  15. MACLEOD R. A. THE QUESTION OF THE EXISTENCE OF SPECIFIC MARINE BACTERIA. Bacteriol Rev. 1965 Mar;29:9–24. [PMC free article] [PubMed] [Google Scholar]
  16. MANDELL J. D., HERSHEY A. D. A fractionating column for analysis of nucleic acids. Anal Biochem. 1960 Jun;1:66–77. doi: 10.1016/0003-2697(60)90020-8. [DOI] [PubMed] [Google Scholar]
  17. MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol. 1962 Jul;5:109–118. doi: 10.1016/s0022-2836(62)80066-7. [DOI] [PubMed] [Google Scholar]
  18. MARMUR J., FALKOW S., MANDEL M. NEW APPROACHES TO BACTERIAL TAXONOMY. Annu Rev Microbiol. 1963;17:329–372. doi: 10.1146/annurev.mi.17.100163.001553. [DOI] [PubMed] [Google Scholar]
  19. MCINTYRE O. R., FEELEY J. C., GREENOUGH W. B., 3rd, BENENSON A. S., HASSAN S. I., SAAD A. DIARRHEA CAUSED BY NON-CHOLERA VIBRIOS. Am J Trop Med Hyg. 1965 May;14:412–418. doi: 10.4269/ajtmh.1965.14.412. [DOI] [PubMed] [Google Scholar]
  20. MIYAZAWA Y., THOMAS C. A., Jr NUCLEOTIDE COMPOSITION OF SHORT SEGMENTS OF DNA MOLECULES. J Mol Biol. 1965 Feb;11:223–237. doi: 10.1016/s0022-2836(65)80053-5. [DOI] [PubMed] [Google Scholar]
  21. Mandel M. New approaches to bacterial taxonomy: perspective and prospects. Annu Rev Microbiol. 1969;23:239–274. doi: 10.1146/annurev.mi.23.100169.001323. [DOI] [PubMed] [Google Scholar]
  22. Mandel M., Weeks O. B., Colwell R. R. Deoxyribonucleic acid base composition of Pseudomonas piscicida. J Bacteriol. 1965 Nov;90(5):1492–1493. doi: 10.1128/jb.90.5.1492-1493.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Martin M. A., Hoyer B. H. Thermal stabilities and species specificities of reannealed animal deoxyribonucleic acids. Biochemistry. 1966 Aug;5(8):2706–2713. doi: 10.1021/bi00872a030. [DOI] [PubMed] [Google Scholar]
  24. McIntyre O. R., Feeley J. C. Characteristics of non-cholera Vibrios isolated from cases of human diarrhoea. Bull World Health Organ. 1965;32(5):627–632. [PMC free article] [PubMed] [Google Scholar]
  25. SAKAZAKI R., IWANAMI S., FUKUMI H. STUDIES ON THE ENTEROPATHOGENIC, FACULTATIVELY HALOPHILIC BACTERIA, VIBRIO PARAHAEMOLYTICUS. I. MORPHOLOGICAL, CULTURAL AND BIOCHEMICAL PROPERTIES AND ITS TAXONOMICAL POSITION. Jpn J Med Sci Biol. 1963 Aug;16:161–188. doi: 10.7883/yoken1952.16.161. [DOI] [PubMed] [Google Scholar]
  26. SCHILDKRAUT C. L., MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J Mol Biol. 1962 Jun;4:430–443. doi: 10.1016/s0022-2836(62)80100-4. [DOI] [PubMed] [Google Scholar]
  27. SEBALD M., VERON M. TENEUR EN BASES DE L'ADN ET CLASSIFICATION DES VIBRIONS. Ann Inst Pasteur (Paris) 1963 Nov;105:897–910. [PubMed] [Google Scholar]
  28. Sakazaki R. Proposal of Vibrio alginolyticus for the biotype 2 of Vibrio parahaemolyticus. Jpn J Med Sci Biol. 1968 Oct;21(5):359–362. doi: 10.7883/yoken1952.21.359. [DOI] [PubMed] [Google Scholar]
  29. TYLER M. E., BIELLING M. C., PRATT D. B. Mineral requirements and other characters of selected marine bacteria. J Gen Microbiol. 1960 Aug;23:153–161. doi: 10.1099/00221287-23-1-153. [DOI] [PubMed] [Google Scholar]
  30. Twedt R. M., Spaulding P. L., Hall H. E. Morphological, cultural, biochemical, and serological comparison of Japanese strains of Vibrio parahemolyticus with related cultures isolated in the United States. J Bacteriol. 1969 May;98(2):511–518. doi: 10.1128/jb.98.2.511-518.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. WOHLHIETER J. A., FALKOW S., CITARELLA R. V., BARON L. S. CHARACTERIZATION OF DNA FROM A PROTEUS STRAIN HARBORING AN EPISOME. J Mol Biol. 1964 Aug;9:576–588. doi: 10.1016/s0022-2836(64)80228-x. [DOI] [PubMed] [Google Scholar]
  32. el Shawi N., Thewaini A. J. Non-agglutinable vibrios isolated in the 1966 epidemic of cholera in Irag. Bull World Health Organ. 1969;40(1):163–166. [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES