Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1970 Oct;104(1):581–584. doi: 10.1128/jb.104.1.581-584.1970

Cytochemical Localization of Catalase Activity in Yeast Peroxisomes

H-P Hoffmann 1, A Szabo 1, C J Avers 1
PMCID: PMC248244  PMID: 5473910

Abstract

Diaminobenzidine oxidation product occurred in peroxisomes and in the intracristate spaces of mitochondria. The reaction was inhibited only in peroxisomes when 3-amino-1,2,4-triazole was present, but cyanide and azide inhibited deposition in both kinds of organelles.

Full text

PDF
582

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AVERS C. J., PFEFFER C. R., RANCOURT M. W. ACRIFLAVINE INDUCTION OF DIFFERENT KINDS OF "PETITE" MITOCHONDRIAL POPULATIONS IN SACCHAROMYCES CEREVISIAE. J Bacteriol. 1965 Aug;90:481–494. doi: 10.1128/jb.90.2.481-494.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Avers C. J. Distribution of cytochrome c peroxidase activity in wild-type and petite cells of bakers' yeast grown aerobically and anaerobically. J Bacteriol. 1967 Oct;94(4):1225–1235. doi: 10.1128/jb.94.4.1225-1235.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Avers C. J., Federman M. The occurrence in yeast of cytoplasmic granules which resemble microbodies. J Cell Biol. 1968 May;37(2):555–559. doi: 10.1083/jcb.37.2.555. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Beard M. E., Novikoff A. B. Distribution of peroxisomes (microbodies) in the nephron of the rat: a cytochemical study. J Cell Biol. 1969 Aug;42(2):501–518. doi: 10.1083/jcb.42.2.501. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. De Duve C., Baudhuin P. Peroxisomes (microbodies and related particles). Physiol Rev. 1966 Apr;46(2):323–357. doi: 10.1152/physrev.1966.46.2.323. [DOI] [PubMed] [Google Scholar]
  6. Fahimi H. D. Cytochemical localization of peroxidatic activity of catalase in rat hepatic microbodies (peroxisomes). J Cell Biol. 1969 Nov;43(2):275–288. doi: 10.1083/jcb.43.2.275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Frederick S. E., Newcomb E. H. Cytochemical localization of catalase in leaf microbodies (peroxisomes). J Cell Biol. 1969 Nov;43(2):343–353. doi: 10.1083/jcb.43.2.343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Graham R. C., Jr, Karnovsky M. J. The histochemical demonstration of uricase activity. J Histochem Cytochem. 1965 Jul-Aug;13(6):448–453. doi: 10.1177/13.6.448. [DOI] [PubMed] [Google Scholar]
  9. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. NAGAI S. Induction of the respiration-deficient mutation in yeast by various synthetic dyes. Science. 1959 Oct 30;130(3383):1188–1189. doi: 10.1126/science.130.3383.1188-a. [DOI] [PubMed] [Google Scholar]
  11. Neal W. K., Hoffmann H. P., Avers C. J., Price C. A. Derepression of mitochondria in yeast spheroplasts. Biochem Biophys Res Commun. 1970 Feb 6;38(3):414–422. doi: 10.1016/0006-291x(70)90729-1. [DOI] [PubMed] [Google Scholar]
  12. Novikoff A. B., Goldfischer S. Visualization of peroxisomes (microbodies) and mitochondria with diaminobenzidine. J Histochem Cytochem. 1969 Oct;17(10):675–680. doi: 10.1177/17.10.675. [DOI] [PubMed] [Google Scholar]
  13. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Szabo A. S., Avers C. J. Some aspects of regulation of peroxisomes and mitochondria in yeast. Ann N Y Acad Sci. 1969 Dec 19;168(2):302–312. doi: 10.1111/j.1749-6632.1969.tb43117.x. [DOI] [PubMed] [Google Scholar]
  15. Yonetani T., Ohnishi T. Cytochrome c peroxidase, a mitochondrial enzyme of yeast. J Biol Chem. 1966 Jun 25;241(12):2983–2984. [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES