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. 1971 Jan;105(1):150–155. doi: 10.1128/jb.105.1.150-155.1971

Evidence for a Magnesium Pump in Bacillus cereus T

Gale B Schmidt 1,2, C L Rosano 1,2, C Hurwitz 1,2
PMCID: PMC248334  PMID: 4993319

Abstract

Unlike Escherichia coli, Bacillus cereus T appears to accumulate Mg2+ in its cell sap against a concentration gradient. Over a range of Mg2+ in the growth medium from 5 × 10−5 to 1.35 × 10−2m, the concentration of Mg2+ in the cell sap of B. cereus T was maintained at about 6 × 10−3m, and ribosome-bound Mg2+ and spermidine, as well as the spermidine concentration in the cell sap, appear to be unaffected by the concentration of Mg2+ in the growth medium. Inhibition of growth of E. coli by streptomycin is progressively reversed by increasing the concentration of Mg2+ in the growth medium above 5 mm. The finding that similar increases of Mg2+ in the growth medium did not reverse the inhibition of B. cereus T is also consistent with the conclusion that B. cereus T, unlike E. coli, accumulates Mg2+ to a constant concentration in its cell sap.

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Selected References

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