Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1971 Jan;105(1):190–199. doi: 10.1128/jb.105.1.190-199.1971

Effects of Essential Amino Acid Starvation in Streptococcus faecalis: Structural Change in the 50S Ribosomal Subunit

R J Ziegler a,1, L Daneo-Moore a
PMCID: PMC248341  PMID: 4925120

Abstract

The 50S ribosomal subunits obtained from exponential-phase and valine-limited cultures of Streptococcus faecalis 9790 differed in protein content. Approximately 35% of the valine-limited subunits showed protein losses when analyzed by CsCl density centrifugation after formaldehyde fixation. Results of density gradient analysis and of acrylamide gel electrophoresis localized the protein to a “split” subunit fraction. No differences could be detected in the protein contents of 30S subunits from exponential-phase and valine-limited cultures.

Full text

PDF
193

Images in this article

197Image
on p.197
198Image
on p.198

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Britten R. J., Roberts R. B. High-Resolution Density Gradient Sedimentation Analysis. Science. 1960 Jan 1;131(3392):32–33. doi: 10.1126/science.131.3392.32. [DOI] [PubMed] [Google Scholar]
  2. Gesteland R. F., Staehelin T. Electrophoretic analysis of proteins from normal and cesium chloride-treated Escherichia coli ribosomes. J Mol Biol. 1967 Mar 14;24(2):149–155. doi: 10.1016/0022-2836(67)90323-3. [DOI] [PubMed] [Google Scholar]
  3. LEBOY P. S., COX E. C., FLAKS J. G. THE CHROMOSOMAL SITE SPECIFYING A RIBOSOMAL PROTEIN IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1964 Dec;52:1367–1374. doi: 10.1073/pnas.52.6.1367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  5. Lucas-Lenard J., Lipmann F. Separation of three microbial amino acid polymerization factors. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1562–1566. doi: 10.1073/pnas.55.6.1562. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. MESELSON M., NOMURA M., BRENNER S., DAVERN C., SCHLESSINGER D. CONSERVATION OF RIBOSOMES DURING BACTERIAL GROWTH. J Mol Biol. 1964 Sep;9:696–711. doi: 10.1016/s0022-2836(64)80176-5. [DOI] [PubMed] [Google Scholar]
  7. Moore L. D., Shockman G. D. Accumulation of an inhibitor of protein synthesis after valine starvation. Biochem Biophys Res Commun. 1966 Aug 23;24(4):541–548. doi: 10.1016/0006-291x(66)90354-8. [DOI] [PubMed] [Google Scholar]
  8. Moore L. D., Umbreit W. W. Membrane-associated protein synthesis in Streptococcus faecalis. Biochim Biophys Acta. 1965 Jul 15;103(3):466–477. doi: 10.1016/0005-2787(65)90139-5. [DOI] [PubMed] [Google Scholar]
  9. Moss B., Ingram V. M. The repression and induction by thyroxin of hemoglobin synthesis during amphibian metamorphosis. Proc Natl Acad Sci U S A. 1965 Sep;54(3):967–974. doi: 10.1073/pnas.54.3.967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nomura M., Traub P. Structure and function of Escherichia coli ribosomes. 3. Stoichiometry and rate of the reconstitution of ribosomes from subribosomal particles and split proteins. J Mol Biol. 1968 Jun 28;34(3):609–619. doi: 10.1016/0022-2836(68)90184-8. [DOI] [PubMed] [Google Scholar]
  11. Otaka E., Itoh T., Osawa S. Ribosomal proteins of bacterial cells: strain- and species-specificity. J Mol Biol. 1968 Apr 14;33(1):93–107. doi: 10.1016/0022-2836(68)90283-0. [DOI] [PubMed] [Google Scholar]
  12. Parenti-Rosina R., Eisenstadt A., Eisenstadt J. M. Isolation of protein initiation factors from 30S ribosomal subunits. Nature. 1969 Jan 25;221(5178):363–365. doi: 10.1038/221363a0. [DOI] [PubMed] [Google Scholar]
  13. Perry R. P., Kelley D. E. Buoyant densities of cytoplasmic ribonucleoprotein particles of mammalian cells: distinctive character of ribosome subunits and the rapidly labeled components. J Mol Biol. 1966 Apr;16(2):255–268. doi: 10.1016/s0022-2836(66)80171-7. [DOI] [PubMed] [Google Scholar]
  14. Spirin A. S., Belitsina N. V., Lerman M. I. Use of formaldehyde fixation for studies of ribonucleoprotein particles by caesium chloride density-gradient centrifugation. J Mol Biol. 1965 Dec;14(2):611–615. doi: 10.1016/s0022-2836(65)80213-3. [DOI] [PubMed] [Google Scholar]
  15. Sussman A. J., Gilvarg C. Protein turnover in amino acid-starved strains of Escherichia coli K-12 differing in their ribonucleic acid control. J Biol Chem. 1969 Nov 25;244(22):6304–6306. [PubMed] [Google Scholar]
  16. Takanami M. Analysis of the 5'-terminal nucleotide sequences of ribonucleic acids 1. the 5'-termini of Excherichia coli ribosomal RNA. J Mol Biol. 1967 Jan 28;23(2):135–148. doi: 10.1016/s0022-2836(67)80022-6. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES