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. 1989 Apr;63(4):1525–1530. doi: 10.1128/jvi.63.4.1525-1530.1989

Inhibition of T-lymphocyte mitogenic responses and effects on cell functions by bovine herpesvirus 1.

J J Carter 1, A D Weinberg 1, A Pollard 1, R Reeves 1, J A Magnuson 1, N S Magnuson 1
PMCID: PMC248384  PMID: 2538643

Abstract

The mitogenic response of bovine peripheral blood mononuclear cells stimulated by concanavalin A (ConA) was suppressed by infectious bovine herpesvirus 1 (BHV-1). Proliferation in response to interleukin-2 (IL-2) by IL-2-dependent lymphocyte cultures was also inhibited by BHV-1. Although inhibition of mitogenesis approached 100%, less than 1 cell in 1,000 was productively infected by BHV-1 in ConA-stimulated cultures. Neither conditioned medium from mitogen-stimulated peripheral blood mononuclear cell cultures nor human recombinant IL-2 reversed suppression by the virus. Infection by BHV-1 did not influence the expression of IL-2 or IL-2 receptor mRNA in ConA-stimulated cultures, nor did it affect the cytolytic capabilities of lymphocytes. The data suggest that the inhibition of T-lymphocyte proliferation is the result of a nonproductive BHV-1 infection.

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Selected References

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