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. 1971 Mar;105(3):908–912. doi: 10.1128/jb.105.3.908-912.1971

Effect of Nalidixic Acid on Semiconservative Replication and Repair Synthesis After Ultraviolet Irradiation in Escherichia coli

Helen Eberle 1, Warren Masker 1
PMCID: PMC248517  PMID: 4926687

Abstract

Both semiconservative deoxyribonucleic acid replication and “extensive repair” synthesis, after ultraviolet irradiation, appear to be blocked by nalidixic acid. These findings suggest that the agent(s) responsible for both of these modes of replication, or some necessary common process or structure, is affected by this drug.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ayad S. R., Fox M. The implication of repair processes in the mechanism of DNA integration by lymphoma cells. Int J Radiat Biol Relat Stud Phys Chem Med. 1969 Jul;15(5):445–455. doi: 10.1080/09553006914550731. [DOI] [PubMed] [Google Scholar]
  2. BOYCE R. P., HOWARD-FLANDERS P. RELEASE OF ULTRAVIOLET LIGHT-INDUCED THYMINE DIMERS FROM DNA IN E. COLI K-12. Proc Natl Acad Sci U S A. 1964 Feb;51:293–300. doi: 10.1073/pnas.51.2.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bonhoeffer F. DNA transfer and DNA synthesis during bacterial conjugation. Z Vererbungsl. 1966;98(2):141–149. doi: 10.1007/BF00897186. [DOI] [PubMed] [Google Scholar]
  4. Boyle J. M., Paterson M. C., Setlow R. B. Excision-repair properties of an Escherichia coli mutant deficient in DNA polymerase. Nature. 1970 May 23;226(5247):708–710. doi: 10.1038/226708a0. [DOI] [PubMed] [Google Scholar]
  5. Boyle J. V., Cook T. M., Goss W. A. Mechanism of action of nalidixic acid on Escherichia coli. Vi. Cell-free studies. J Bacteriol. 1969 Jan;97(1):230–236. doi: 10.1128/jb.97.1.230-236.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Deitz W. H., Cook T. M., Goss W. A. Mechanism of action of nalidixic acid on Escherichia coli. 3. Conditions required for lethality. J Bacteriol. 1966 Feb;91(2):768–773. doi: 10.1128/jb.91.2.768-773.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. GOSS W. A., DEITZ W. H., COOK T. M. MECHANISM OF ACTION OF NALIDIXIC ACID ON ESCHERICHIA COLI.II. INHIBITION OF DEOXYRIBONUCLEIC ACID SYNTHESIS. J Bacteriol. 1965 Apr;89:1068–1074. doi: 10.1128/jb.89.4.1068-1074.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gross J. D., Karamata D., Hempstead P. G. Temperature-sensitive mutants of B. subtilis defective in DNA synthesis. Cold Spring Harb Symp Quant Biol. 1968;33:307–312. doi: 10.1101/sqb.1968.033.01.034. [DOI] [PubMed] [Google Scholar]
  9. Hanawalt P. C., Pettijohn D. E., Pauling E. C., Brunk C. F., Smith D. W., Kanner L. C., Couch J. L. Repair replication of DNA in vivo. Cold Spring Harb Symp Quant Biol. 1968;33:187–194. doi: 10.1101/sqb.1968.033.01.022. [DOI] [PubMed] [Google Scholar]
  10. Hane M. W., Wood T. H. Escherichia coli K-12 mutants resistant to nalidixic acid: genetic mapping and dominance studies. J Bacteriol. 1969 Jul;99(1):238–241. doi: 10.1128/jb.99.1.238-241.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hirota Y., Ryter A., Jacob F. Thermosensitive mutants of E. coli affected in the processes of DNA synthesis and cellular division. Cold Spring Harb Symp Quant Biol. 1968;33:677–693. doi: 10.1101/sqb.1968.033.01.077. [DOI] [PubMed] [Google Scholar]
  12. Kanner L., Hanawalt P. Efficiency of utilization of thymine and 5-bromouracil for normal and repair DNA synthesis in bacteria. Biochim Biophys Acta. 1968 May 21;157(3):532–545. doi: 10.1016/0005-2787(68)90151-2. [DOI] [PubMed] [Google Scholar]
  13. LARK K. G., REPKO T., HOFFMAN E. J. THE EFFECT OF AMINO ACID DEPRIVATION ON SUBSEQUENT DEOXYRIBONUCLEIC ACID REPLICATION. Biochim Biophys Acta. 1963 Sep 17;76:9–24. [PubMed] [Google Scholar]
  14. LATARJET R., MORENNE P., BERGER R. Un appareil simple pour le dosage des rayonnements ultraviolets émis par les lampes germicides. Ann Inst Pasteur (Paris) 1953 Aug;85(2):175–184. [PubMed] [Google Scholar]
  15. LEHMAN I. R., BESSMAN M. J., SIMMS E. S., KORNBERG A. Enzymatic synthesis of deoxyribonucleic acid. I. Preparation of substrates and partial purification of an enzyme from Escherichia coli. J Biol Chem. 1958 Jul;233(1):163–170. [PubMed] [Google Scholar]
  16. PETTIJOHN D., HANAWALT P. EVIDENCE FOR REPAIR-REPLICATION OF ULTRAVIOLET DAMAGED DNA IN BACTERIA. J Mol Biol. 1964 Aug;9:395–410. doi: 10.1016/s0022-2836(64)80216-3. [DOI] [PubMed] [Google Scholar]
  17. SETLOW R. B., CARRIER W. L. THE DISAPPEARANCE OF THYMINE DIMERS FROM DNA: AN ERROR-CORRECTING MECHANISM. Proc Natl Acad Sci U S A. 1964 Feb;51:226–231. doi: 10.1073/pnas.51.2.226. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Silver S., Wendt L. Mechanism of action of phenethyl alcohol: breakdown of the cellular permeability barrier. J Bacteriol. 1967 Feb;93(2):560–566. doi: 10.1128/jb.93.2.560-566.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. WULFF D. L., RUPERT C. S. Disappearance of thymine photodimer in ultraviolet irradiated DNA upon treatment with a photoreactivating enzyme from baker's yeast. Biochem Biophys Res Commun. 1962 Apr 20;7:237–240. doi: 10.1016/0006-291x(62)90181-x. [DOI] [PubMed] [Google Scholar]

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