Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1991 Oct;65(10):5485–5490. doi: 10.1128/jvi.65.10.5485-5490.1991

Identification and mapping of neutralizing epitopes of human parvovirus B19 by using human antibodies.

H Sato 1, J Hirata 1, N Kuroda 1, H Shiraki 1, Y Maeda 1, K Okochi 1
PMCID: PMC249042  PMID: 1716693

Abstract

We identified and mapped the regions responsible for neutralization in the human parvovirus B19 structural protein by using region-specific human antibodies derived from seropositive blood donors. The region-specific antibodies were purified by using affinity columns coupled with synthetic peptides of the hydrophilic regions including the beta-turn structure deduced by the predicted secondary structure of VP2. Fifteen highly specific antibodies against the synthetic peptides were obtained. Ten of them were able to precipitate the radiolabeled virus. Six of them proved to be able to protect the colony-forming unit erythroid cells in human bone marrow cell cultures from injury by the virus. The sequences recognized by the six neutralizing antibodies were sites corresponding to amino acids 253 to 272, 309 to 330, 325 to 346, 359 to 382, 449 to 468, and 491 to 515 from the amino-terminal portion of VP2. These observations suggest that the neutralizing epitopes were distributed in the region from amino acid 253 in the amino-terminal portion of VP2 to the carboxyl terminus of VP2.

Full text

PDF
5490

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anand A., Gray E. S., Brown T., Clewley J. P., Cohen B. J. Human parvovirus infection in pregnancy and hydrops fetalis. N Engl J Med. 1987 Jan 22;316(4):183–186. doi: 10.1056/NEJM198701223160403. [DOI] [PubMed] [Google Scholar]
  2. Anderson M. J., Jones S. E., Fisher-Hoch S. P., Lewis E., Hall S. M., Bartlett C. L., Cohen B. J., Mortimer P. P., Pereira M. S. Human parvovirus, the cause of erythema infectiosum (fifth disease)? Lancet. 1983 Jun 18;1(8338):1378–1378. doi: 10.1016/s0140-6736(83)92152-9. [DOI] [PubMed] [Google Scholar]
  3. Brown T., Anand A., Ritchie L. D., Clewley J. P., Reid T. M. Intrauterine parvovirus infection associated with hydrops fetalis. Lancet. 1984 Nov 3;2(8410):1033–1034. doi: 10.1016/s0140-6736(84)91126-7. [DOI] [PubMed] [Google Scholar]
  4. Chou P. Y., Fasman G. D. Prediction of protein conformation. Biochemistry. 1974 Jan 15;13(2):222–245. doi: 10.1021/bi00699a002. [DOI] [PubMed] [Google Scholar]
  5. Clewley J. P. Biochemical characterization of a human parvovirus. J Gen Virol. 1984 Jan;65(Pt 1):241–245. doi: 10.1099/0022-1317-65-1-241. [DOI] [PubMed] [Google Scholar]
  6. Cohen B. J., Mortimer P. P., Pereira M. S. Diagnostic assays with monoclonal antibodies for the human serum parvovirus-like virus (SPLV). J Hyg (Lond) 1983 Aug;91(1):113–130. doi: 10.1017/s0022172400060095. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cossart Y. E., Field A. M., Cant B., Widdows D. Parvovirus-like particles in human sera. Lancet. 1975 Jan 11;1(7898):72–73. doi: 10.1016/s0140-6736(75)91074-0. [DOI] [PubMed] [Google Scholar]
  8. Cotmore S. F., McKie V. C., Anderson L. J., Astell C. R., Tattersall P. Identification of the major structural and nonstructural proteins encoded by human parvovirus B19 and mapping of their genes by procaryotic expression of isolated genomic fragments. J Virol. 1986 Nov;60(2):548–557. doi: 10.1128/jvi.60.2.548-557.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cotmore S. F., Tattersall P. Characterization and molecular cloning of a human parvovirus genome. Science. 1984 Dec 7;226(4679):1161–1165. doi: 10.1126/science.6095448. [DOI] [PubMed] [Google Scholar]
  10. Duncan J. R., Potter C. B., Cappellini M. D., Kurtz J. B., Anderson M. J., Weatherall D. J. Aplastic crisis due to parvovirus infection in pyruvate kinase deficiency. Lancet. 1983 Jul 2;2(8340):14–16. doi: 10.1016/s0140-6736(83)90005-3. [DOI] [PubMed] [Google Scholar]
  11. Knott P. D., Welply G. A., Anderson M. J. Serologically proved intrauterine infection with parvovirus. Br Med J (Clin Res Ed) 1984 Dec 15;289(6459):1660–1660. doi: 10.1136/bmj.289.6459.1660. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kurtzman G. J., Cohen B. J., Field A. M., Oseas R., Blaese R. M., Young N. S. Immune response to B19 parvovirus and an antibody defect in persistent viral infection. J Clin Invest. 1989 Oct;84(4):1114–1123. doi: 10.1172/JCI114274. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kurtzman G. J., Cohen B., Meyers P., Amunullah A., Young N. S. Persistent B19 parvovirus infection as a cause of severe chronic anaemia in children with acute lymphocytic leukaemia. Lancet. 1988 Nov 19;2(8621):1159–1162. doi: 10.1016/s0140-6736(88)90233-4. [DOI] [PubMed] [Google Scholar]
  14. Kurtzman G. J., Ozawa K., Cohen B., Hanson G., Oseas R., Young N. S. Chronic bone marrow failure due to persistent B19 parvovirus infection. N Engl J Med. 1987 Jul 30;317(5):287–294. doi: 10.1056/NEJM198707303170506. [DOI] [PubMed] [Google Scholar]
  15. Kyte J., Doolittle R. F. A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982 May 5;157(1):105–132. doi: 10.1016/0022-2836(82)90515-0. [DOI] [PubMed] [Google Scholar]
  16. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  17. Mortimer P. P., Humphries R. K., Moore J. G., Purcell R. H., Young N. S. A human parvovirus-like virus inhibits haematopoietic colony formation in vitro. 1983 Mar 31-Apr 6Nature. 302(5907):426–429. doi: 10.1038/302426a0. [DOI] [PubMed] [Google Scholar]
  18. Mortimer P. P. Hypothesis: the aplastic crisis of hereditary spherocytosis is due to a single transmissible agent. J Clin Pathol. 1983 Apr;36(4):445–448. doi: 10.1136/jcp.36.4.445. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Okochi K., Mori R., Miyazaki M., Cohen B. J., Mortimer P. P. Nakatani antigen and human parvovirus (B19) Lancet. 1984 Jan 21;1(8369):160–161. doi: 10.1016/s0140-6736(84)90087-4. [DOI] [PubMed] [Google Scholar]
  20. Ozawa K., Ayub J., Hao Y. S., Kurtzman G., Shimada T., Young N. Novel transcription map for the B19 (human) pathogenic parvovirus. J Virol. 1987 Aug;61(8):2395–2406. doi: 10.1128/jvi.61.8.2395-2406.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Parvovirus infection, leukaemia, and immunodeficiency. Lancet. 1989 Jan 14;1(8629):101–102. [PubMed] [Google Scholar]
  22. Pattison J. R., Jones S. E., Hodgson J., Davis L. R., White J. M., Stroud C. E., Murtaza L. Parvovirus infections and hypoplastic crisis in sickle-cell anaemia. Lancet. 1981 Mar 21;1(8221):664–665. doi: 10.1016/s0140-6736(81)91579-8. [DOI] [PubMed] [Google Scholar]
  23. Rao K. R., Patel A. R., Anderson M. J., Hodgson J., Jones S. E., Pattison J. R. Infection with parvovirus-like virus and aplastic crisis in chronic hemolytic anemia. Ann Intern Med. 1983 Jun;98(6):930–932. doi: 10.7326/0003-4819-98-6-930. [DOI] [PubMed] [Google Scholar]
  24. Reid D. M., Reid T. M., Brown T., Rennie J. A., Eastmond C. J. Human parvovirus-associated arthritis: a clinical and laboratory description. Lancet. 1985 Feb 23;1(8426):422–425. doi: 10.1016/s0140-6736(85)91146-8. [DOI] [PubMed] [Google Scholar]
  25. Sato H., Hirata J., Furukawa M., Kuroda N., Shiraki H., Maeda Y., Okochi K. Identification of the region including the epitope for a monoclonal antibody which can neutralize human parvovirus B19. J Virol. 1991 Apr;65(4):1667–1672. doi: 10.1128/jvi.65.4.1667-1672.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Sato H., Okochi K. Transmission of human T-cell leukemia virus (HTLV-I) by blood transfusion: demonstration of proviral DNA in recipients' blood lymphocytes. Int J Cancer. 1986 Mar 15;37(3):395–400. doi: 10.1002/ijc.2910370311. [DOI] [PubMed] [Google Scholar]
  27. Shade R. O., Blundell M. C., Cotmore S. F., Tattersall P., Astell C. R. Nucleotide sequence and genome organization of human parvovirus B19 isolated from the serum of a child during aplastic crisis. J Virol. 1986 Jun;58(3):921–936. doi: 10.1128/jvi.58.3.921-936.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. White D. G., Woolf A. D., Mortimer P. P., Cohen B. J., Blake D. R., Bacon P. A. Human parvovirus arthropathy. Lancet. 1985 Feb 23;1(8426):419–421. doi: 10.1016/s0140-6736(85)91145-6. [DOI] [PubMed] [Google Scholar]
  29. Yaegashi N., Shiraishi H., Takeshita T., Nakamura M., Yajima A., Sugamura K. Propagation of human parvovirus B19 in primary culture of erythroid lineage cells derived from fetal liver. J Virol. 1989 Jun;63(6):2422–2426. doi: 10.1128/jvi.63.6.2422-2426.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES