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. 1969 Jan;97(1):166–173. doi: 10.1128/jb.97.1.166-173.1969

Development of Competence in Thymine-starved Bacillus subtilis with Chromosomes Arrested at the Terminus1

Luis J Archer a,2, Otto E Landman a
PMCID: PMC249570  PMID: 4974387

Abstract

Tryptophan- and thymine-requiring cells of Bacillus subtilis, emerging from an amino acid starvation treatment which causes arrest of the chromosomes at the terminus, were not transformable. During subsequent incubation in a thymineless medium supplemented with amino acids, the cultures developed competence while retaining chromosome arrest. The competent subpopulation apparently shares the synchronous chromosome arrest of the bulk population. This was shown by different methods. The principal method was marker frequency analysis of the deoxyribonucleic acid extracted from a population enriched for competent cells by a column-chromatographic method. It is concluded that development of the competent state can occur in nondividing cells, and that the presence of a replication fork actively engaged in synthesis of deoxyribonucleic acid is not required for the development of this state.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anagnostopoulos C., Spizizen J. REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS. J Bacteriol. 1961 May;81(5):741–746. doi: 10.1128/jb.81.5.741-746.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anraku N., Landman O. E. Control of the synthesis of macromolecules during amino acid and thymine starvation in Bacillus subtilis. J Bacteriol. 1968 May;95(5):1813–1827. doi: 10.1128/jb.95.5.1813-1827.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Archer L. J., Landman O. E. Transport of donor deoxyribonucleic acid into the cell interior of thymine-starved Bacillus subtilis with chromosomes arrested at the terminus. J Bacteriol. 1969 Jan;97(1):174–181. doi: 10.1128/jb.97.1.174-181.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bodmer W. F. Recombination and integration in Bacillus subtilis transformation: involvement of DNA synthesis. J Mol Biol. 1965 Dec;14(2):534–557. doi: 10.1016/s0022-2836(65)80203-0. [DOI] [PubMed] [Google Scholar]
  5. Bott K. F., Wilson G. A. Development of competence in the Bacillus subtilis transformation system. J Bacteriol. 1967 Sep;94(3):562–570. doi: 10.1128/jb.94.3.562-570.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FARMER J. L., ROTHMAN F. TRANSFORMABLE THYMINE-REQUIRING MUTANT OF BACILLUS SUBTILS. J Bacteriol. 1965 Jan;89:262–263. doi: 10.1128/jb.89.1.262-263.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LANDMAN O. E., HALLE S. ENZYMICALLY AND PHYSICALLY INDUCED INHERITANCE CHANGES IN BACILLUS SUBTILIS. J Mol Biol. 1963 Dec;7:721–738. doi: 10.1016/s0022-2836(63)80119-9. [DOI] [PubMed] [Google Scholar]
  8. Lark K. G. Regulation of chromosome replication and segregation in bacteria. Bacteriol Rev. 1966 Mar;30(1):3–32. doi: 10.1128/br.30.1.3-32.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McCarthy C., Nester E. W. Macromolecular synthesis in newly transformed cells of Bacillus subtilis. J Bacteriol. 1967 Jul;94(1):131–140. doi: 10.1128/jb.94.1.131-140.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. NESTER E. W. PENICILLIN RESISTANCE OF COMPETENT CELLS IN DEOXYRIBONUCLEIC ACID TRANSFORMATION OF BACILLUS SUBTILIS. J Bacteriol. 1964 Apr;87:867–875. doi: 10.1128/jb.87.4.867-875.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. SAITO H., MIURA K. I. PREPARATION OF TRANSFORMING DEOXYRIBONUCLEIC ACID BY PHENOL TREATMENT. Biochim Biophys Acta. 1963 Aug 20;72:619–629. [PubMed] [Google Scholar]
  12. Spizizen J. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072–1078. doi: 10.1073/pnas.44.10.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. YOSHIKAWA H., O'SULLIVAN A., SUEOKA N. SEQUENTIAL REPLICATION OF THE BACILLUS SUBTILIS CHROMOSOME. 3. REGULATION OF INITIATION. Proc Natl Acad Sci U S A. 1964 Oct;52:973–980. doi: 10.1073/pnas.52.4.973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. YOSHIKAWA H., SUEOKA N. Sequential replication of the Bacillus subtilis chromosome. II. Isotopic transfer experiments. Proc Natl Acad Sci U S A. 1963 Jun;49:806–813. doi: 10.1073/pnas.49.6.806. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Young F. E. Competence in Bacillus subtilis transformation system. Nature. 1967 Feb 25;213(5078):773–775. doi: 10.1038/213773a0. [DOI] [PubMed] [Google Scholar]

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