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. 1969 Feb;97(2):612–619. doi: 10.1128/jb.97.2.612-619.1969

Effect of pH on the Immunogenicity of Mycoplasma pneumoniae

J Dennis Pollack 1, Norman L Somerson 2, Laurence B Senterfit 3
PMCID: PMC249735  PMID: 4975150

Abstract

Mycoplasma pneumoniae harvested from media which had become acid lost the ability both to induce formation of tetrazolium reduction inhibition antibody and to act as antigens in immunodiffusion against human convalescent-phase sera. Incorporation of N-tris(hydroxymethyl)-2-aminoethane sulfonic acid and N-2-hydroxyethylpiperazine-N′-2-ethane sulfonic acid buffers into a new medium containing PPLO Serum Fraction instead of horse serum delayed the pH decline. Tris(hydroxymethyl)aminomethane, triethanolamine, and 3,6-endomethylene-1,2,3,6-tetrahydrophthalic acid buffers inhibited growth. Mycoplasmas obtained from buffered cultures retained antigenicity as measured by immunodiffusion and could stimulate tetrazolium reduction inhibition antibody formation in animals.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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