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. 1969 Feb;97(2):867–872. doi: 10.1128/jb.97.2.867-872.1969

Gene-Enzyme Relationships in Neurospora Invertase1

Malcolm L Sargent a,2, Dow O Woodward a
PMCID: PMC249771  PMID: 4975154

Abstract

A spontaneous, single-gene mutation responsible for a total lack of invertase activity in Neurospora crassa is described. The mutation is believed to lie in the structural gene for invertase, since an immunologically cross-reacting protein is made by the mutant strain. In addition, there was no evidence for a defect in regulation of invertase activity or synthesis by the following criteria. (i) The invertaseless condition was recessive in heterokaryons; (ii) no invertase inhibitor was found in mutant extracts by mixing experiments; and (iii) none of the several sugars able to induce activity in wild-type strains was able to induce activity in the mutant strain. It was also discovered that most of the wild-type enzyme (55 to 75%) cannot be washed free from the rapidly sedimenting cell debris. This finding provided additional support for the hypothesis that Neurospora invertase is located within or about the cell wall.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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