Abstract
Methyl viologen was reduced photochemically in the presence of proflavine and ethylenediaminetetraacetic acid. The reduced methyl viologen was oxidized by hydrogenase from Vibrio succinogenes. H2 and oxidized methyl viologen were the products. Hydrogenase activity was determined by spectrophotometric measurement of the disappearance of reduced methyl viologen at 600 nm. The extinction coefficient of reduced methyl viologen was determined and is 8.25 mm−1 × cm−1 at 600 nm. Optimal conditions for assaying V. succinogenes hydrogenase were developed. Extracts of Escherichia coli and Desulfovibrio desulfuricans catalyzed reduced methyl viologen oxidation in the assay system, whereas Azotobacter vinelandii extracts were inactive.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Aspen A. J., Wolin M. J. Solubilization and reconstitution of a particulate hydrogenase from Vibrio succinogenes. J Biol Chem. 1966 Sep 25;241(18):4152–4156. [PubMed] [Google Scholar]
- GEST H., PECK H. D., Jr A study of the hydrogenlyase reaction with systems derived from normal and anaerogenic coli-aerogenes bacteria. J Bacteriol. 1955 Sep;70(3):326–334. doi: 10.1128/jb.70.3.326-334.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- PECK H. D., Jr, GEST H. A new procedure for assay of bacterial hydrogenases. J Bacteriol. 1956 Jan;71(1):70–80. doi: 10.1128/jb.71.1.70-80.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PECK H. D., Jr, GEST H. Formic dehydrogenase and the hydrogenlyase enzyme complex in coli-aerogenes bacteria. J Bacteriol. 1957 Jun;73(6):706–721. doi: 10.1128/jb.73.6.706-721.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RUFENER W. H., Jr, NELSON W. O., WOLIN M. J. Maintenance of the rumen microbial population in continuous culture. Appl Microbiol. 1963 May;11:196–201. doi: 10.1128/am.11.3.196-201.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Strandberg G. W., Wilson P. W. Molecular H2 and the PN2 function of azotobacter. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1404–1409. doi: 10.1073/pnas.58.4.1404. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wolin M. J. Lysis of Vibrio succinogenes by ethylenediamine-tetraacetic acid or lysozyme. J Bacteriol. 1966 May;91(5):1781–1786. doi: 10.1128/jb.91.5.1781-1786.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]