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. 1969 Apr;98(1):56–61. doi: 10.1128/jb.98.1.56-61.1969

Regulation of Nitrogen Fixation in Azotobacter vinelandii OP: the Role of Nitrate Reductase

George J Sorger 1
PMCID: PMC249903  PMID: 5781587

Abstract

A number of chlorate-resistant mutants were selected, and one of these, clr68-5, was studied in detail. This mutant cannot utilize nitrate in vivo to overcome the effect of nonmetabolizable repressors of nitrogenase. The reason for this inability was that strain clr68-5 lacked nitrate reductase. Nitrate inhibited the activity of nitrogenase but did not act as a corepressor of nitrogenase in strain clr68-5 as it does in the wild type. Ammonia seemed to act as corepressor of nitrogenase in both strains.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BULEN W. A., BURNS R. C., LECOMTE J. R. NITROGEN FIXATION: HYDROSULFITE AS ELECTRON DONOR WITH CELL-FREE PREPARATIONS OF AZOTOBACTER VINELANDII AND RHODOSPIRILLUM RUBRUM. Proc Natl Acad Sci U S A. 1965 Mar;53:532–539. doi: 10.1073/pnas.53.3.532. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DE WITT C. W., ROWE J. A. N,O-Diacetylneuraminic acid and N-acetylneuraminic acid in Escherichia coli. Nature. 1959 Aug 1;184(Suppl 6):381–382. doi: 10.1038/184381b0. [DOI] [PubMed] [Google Scholar]
  3. GLOCK G. E., McLEAN P. Further studies on the properties and assay of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase of rat liver. Biochem J. 1953 Oct;55(3):400–408. doi: 10.1042/bj0550400. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. GREEN M., WILSON P. W. Hydrogenase and nitrogenase in Azotobacter. J Bacteriol. 1953 May;65(5):511–517. doi: 10.1128/jb.65.5.511-517.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GREEN M., WILSON P. W. The utilization of nitrate nitrogen by the Azotobacter. J Gen Microbiol. 1953 Aug;9(1):89–96. doi: 10.1099/00221287-9-1-89. [DOI] [PubMed] [Google Scholar]
  6. HACKENTHAL E., MANNHEIM W., HACKENTHAL R., BECHER R. DIE REDUKTION VON PERCHLORAT DURCH BAKTERIEN. I. UNTERSUCHUNGEN AN INTAKTEN ZELLEN. Biochem Pharmacol. 1964 Feb;13:195–206. doi: 10.1016/0006-2952(64)90137-6. [DOI] [PubMed] [Google Scholar]
  7. HAMILTON I. R., BURRIS R. H., WILSON P. W. HYDROGENASE AND NITROGENASE IN A NITROGEN-FIXING BACTERIUM. Proc Natl Acad Sci U S A. 1964 Sep;52:637–641. doi: 10.1073/pnas.52.3.637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hardy R. W., Knight E., Jr, D'Eustachio A. J. An energy-dependent hydrogen-evolution from dithionite in nitrogen-fixing extracts of Clostridium pasteurianum. Biochem Biophys Res Commun. 1965 Sep 8;20(5):539–544. doi: 10.1016/0006-291x(65)90431-6. [DOI] [PubMed] [Google Scholar]
  9. LOWE R. H., EVANS H. J. PREPARATION AND SOME PROPERTIES OF A SOLUBLE NITRATE REDUCTASE FROM RHIZOBIUM JAPONICUM. Biochim Biophys Acta. 1964 Jun 1;85:377–389. doi: 10.1016/0926-6569(64)90301-3. [DOI] [PubMed] [Google Scholar]
  10. Mahl M. C., Wilson P. W. Nitrogen fixation by cell-free extracts of Klebsiella penumoniae. Can J Microbiol. 1968 Jan;14(1):33–38. doi: 10.1139/m68-006. [DOI] [PubMed] [Google Scholar]
  11. Mortenson L. E., Morris J. A., Jeng D. Y. Purification, metal composition and properties of molybdoferredoxin and azoferredoxin, two of the components of the nitrogen-fixing system of Clostridium pasteurianum. Biochim Biophys Acta. 1967 Aug 29;141(3):516–522. doi: 10.1016/0304-4165(67)90180-8. [DOI] [PubMed] [Google Scholar]
  12. NICHOLAS D. J., WILSON P. W., HEINEN W., PALMER G., BEINERT H. Use of electron paramagnetic resonance spectroscopy in investigations of functional metal components in micro-organisms. Nature. 1962 Nov 3;196:433–436. doi: 10.1038/196433a0. [DOI] [PubMed] [Google Scholar]
  13. PICHINOTY F. A PROPOS DES NITRATE-R'EDUCTASES D'UNE BACT'ERIE D'ENITRIFIANTE. Biochim Biophys Acta. 1964 Aug 26;89:378–381. [PubMed] [Google Scholar]
  14. PICHINOTY F. [Research on nitrate-reductase of Aerobacter aerogenes. Properties, specificity of action and inhibition by oxygen]. Ann Inst Pasteur (Paris) 1963 Mar;104:394–418. [PubMed] [Google Scholar]
  15. Piéchaud M., Puig J., Pichinoty F., Azoulay E., Le Minor L. Mutations affectant la nitrate-réductase A et d'autres enzymes bactériennes d'oxydoréduction. Ann Inst Pasteur (Paris) 1967 Jan;112(1):24–37. [PubMed] [Google Scholar]
  16. Sorger G. J., Giles N. H. Genetic control of nitrate reductase in Neurospora crassa. Genetics. 1965 Oct;52(4):777–788. doi: 10.1093/genetics/52.4.777. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sorger G. J. Regulation of nitrogen fixation in Azotobacter vinelandii OP and in an apparently partially constitutive mutant. J Bacteriol. 1968 May;95(5):1721–1726. doi: 10.1128/jb.95.5.1721-1726.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Strandberg G. W., Wilson P. W. Formation of the nitrogen-fixing enzyme system in Azotobacter vinelandii. Can J Microbiol. 1968 Jan;14(1):25–31. doi: 10.1139/m68-005. [DOI] [PubMed] [Google Scholar]
  19. Wilson P. W., Burris R. H. THE MECHANISM OF BIOLOGICAL NITROGEN FIXATION. Bacteriol Rev. 1947 Mar;11(1):41–73. doi: 10.1128/br.11.1.41-73.1947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wilson P. W., Hull J. F., Burris R. H. Competition between Free and Combined Nitrogen in Nutrition of Azotobacter. Proc Natl Acad Sci U S A. 1943 Sep;29(9):289–294. doi: 10.1073/pnas.29.9.289. [DOI] [PMC free article] [PubMed] [Google Scholar]

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