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. 1969 Nov;100(2):803–808. doi: 10.1128/jb.100.2.803-808.1969

Circular Deoxyribonucleic Acid from Shigella dysenteriae Y6R

Mark G Rush a,1, C N Gordon a, Robert C Warner a,2
PMCID: PMC250161  PMID: 4901361

Abstract

Circular deoxyribonucleic acid was isolated from Shigella dysenteriae Y6R and was found to consist of six species having molecular weights of 106, 1.3 × 106, 2.6 × 106, 3.8 × 106, 20 × 106, and 24 × 106 daltons. These size classes were partially resolved by sucrose density gradient centrifugation. The minicircles (106 and 1.3 × 106) were found to have a buoyant density in CsCl of 1.710 g/ml. The 3.8 × 106 dalton class had a density of 1.707 g/ml. The two largest species had a density of 1.702 g/ml. Two other strains, S. sonnei II and S. dysenteriae 60, also contained circular deoxyribonucleic acid.

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Selected References

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  1. Ansz H. S., Zandberg J., van de Pol J. H., van Bruggen E. F. Circular DNA from Shigella paradysenteriae. Eur J Biochem. 1969 Jun;9(2):156–159. doi: 10.1111/j.1432-1033.1969.tb00589.x. [DOI] [PubMed] [Google Scholar]
  2. Cozzarelli N. R., Kelly R. B., Kornberg A. A minute circular DNA from Escherichia coli 15. Proc Natl Acad Sci U S A. 1968 Jul;60(3):992–999. doi: 10.1073/pnas.60.3.992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Freifelder D. Studies on Escherichia coli sex factors. IV. Molecular weights of the DNA of several F' elements. J Mol Biol. 1968 Jul 14;35(1):95–102. doi: 10.1016/s0022-2836(68)80039-7. [DOI] [PubMed] [Google Scholar]
  4. Hickson F. T., Roth T. F., Helinski D. R. Circular DNA forms of a bacterial sex factor. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1731–1738. doi: 10.1073/pnas.58.4.1731. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lee C. S., Davidson N. Covalently closed minicircular DNA in Micrococcus lysodeikticus. Biochem Biophys Res Commun. 1968 Sep 6;32(5):757–762. doi: 10.1016/0006-291x(68)90304-5. [DOI] [PubMed] [Google Scholar]
  6. Novick R. P. Extrachromosomal inheritance in bacteria. Bacteriol Rev. 1969 Jun;33(2):210–263. doi: 10.1128/br.33.2.210-263.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Novick R. P., Roth C. Plasmid-linked resistance to inorganic salts in Staphylococcus aureus. J Bacteriol. 1968 Apr;95(4):1335–1342. doi: 10.1128/jb.95.4.1335-1342.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Poulwels P. H., Jansz H. S., van Rotterdam J., Cohen J. A. Structure of the replicative form of bacteriophage phi-X-174. Physico-chemical studies. Biochim Biophys Acta. 1966 May 19;119(2):289–300. doi: 10.1016/0005-2787(66)90187-0. [DOI] [PubMed] [Google Scholar]
  9. Radloff R., Bauer W., Vinograd J. A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells. Proc Natl Acad Sci U S A. 1967 May;57(5):1514–1521. doi: 10.1073/pnas.57.5.1514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Riou G., Paoletti C. Preparation and properties of nuclear and satellite deoxyribonucleic acid of Trypanosoma cruzi. J Mol Biol. 1967 Sep 14;28(2):377–382. doi: 10.1016/s0022-2836(67)80017-2. [DOI] [PubMed] [Google Scholar]
  11. Roth T. F., Helinski D. R. Evidence for circular DNA forms of a bacterial plasmid. Proc Natl Acad Sci U S A. 1967 Aug;58(2):650–657. doi: 10.1073/pnas.58.2.650. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rownd R., Nakaya R., Nakamura A. Molecular nature of the drug-resistance factors of the Enterobacteriaceae. J Mol Biol. 1966 Jun;17(2):376–393. doi: 10.1016/s0022-2836(66)80149-3. [DOI] [PubMed] [Google Scholar]
  13. Rush M. G., Kleinschmidt A. K., Hellmann W., Warner R. C. Multiple-length rings in preparations of phi-X174 replicative form. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1676–1683. doi: 10.1073/pnas.58.4.1676. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rush M. G., Warner R. C. Multiple-length rings of phi-X-174 replicative form, II. Infectivity. Proc Natl Acad Sci U S A. 1967 Dec;58(6):2372–2376. doi: 10.1073/pnas.58.6.2372. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Vinograd J., Lebowitz J. Physical and topological properties of circular DNA. J Gen Physiol. 1966 Jul;49(6):103–125. doi: 10.1085/jgp.49.6.103. [DOI] [PMC free article] [PubMed] [Google Scholar]

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