Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1969 Dec;100(3):1284–1288. doi: 10.1128/jb.100.3.1284-1288.1969

Killing of Haemophilus influenzae Cells by Integrated Ultraviolet-Induced Lesions from Transforming Deoxyribonucleic Acid

Kenneth L Beattie 1, Jane K Setlow 1
PMCID: PMC250315  PMID: 5308034

Abstract

Highly competent cultures of Haemophilus influenzae are inactivated by exposure to transforming deoxyribonucleic acid (DNA) irradiated with ultraviolet light (UV). As a function of UV dose to the DNA, the killing goes to a maximum and then decreases. The killing of H. influenzae by unirradiated H. parainfluenzae DNA, reported by other workers, is enhanced by low doses of UV, but drops off at high doses. Since there are no such lethal effects in a strain of H. influenzae that takes up DNA normally but does not integrate it, it is concluded that the killing is associated with integrated UV lesions. All the killing of wild-type cells due to irradiated DNA is eliminated by photoreactivation of the DNA. The killing of an excisionless strain of H. influenzae, however, is not eliminated by maximal photoreactivation of the irradiated transforming DNA. The nonphotoreactivable fraction of killing in the excisionless strain increases with increasing dose. The kinetics of the killing-dose curves may be explained only partially in terms of UV-induced loss of integration. It is postulated that the number of pyrimidine dimers relative to other DNA components integrated decreases at higher UV doses.

Full text

PDF
1287

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boling M. E., Setlow J. K. Dependence of Vegetative Recombination Among Haemophilus influenzae Bacteriophage on the Host Cell. J Virol. 1969 Sep;4(3):240–243. doi: 10.1128/jvi.4.3.240-243.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CABRERA JUAREZ E., HERRIOTT R. M. ULTRAVIOLET IRRADIATION OF NATIVE AND DENATURED TRANSFORMING DEOXYRIBONUCLEIC ACID FROM HAEMOPHILUS INFLUENZAE. J Bacteriol. 1963 Mar;85:671–675. doi: 10.1128/jb.85.3.671-675.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Modak S. P., Setlow J. K. Synthesis of deoxyribonucleic acid after ultraviolet irradiation of sensitive and resistant Haemophilus influenzae. J Bacteriol. 1969 Jun;98(3):1195–1198. doi: 10.1128/jb.98.3.1195-1198.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Muhammed A. Studies on the yeast photoreactivating enzyme. I. A method for the large scale purification and some properties of the enzyme. J Biol Chem. 1966 Jan 25;241(2):516–523. [PubMed] [Google Scholar]
  5. Notani N., Goodgal S. H. On the nature of recombinants formed during transformation in Hemophilus influenzae. J Gen Physiol. 1966 Jul;49(6):197–209. doi: 10.1085/jgp.49.6.197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Setlow J. K., Boling M. E., Bollum F. J. The chemical nature of photoreactivable lesions in DNA. Proc Natl Acad Sci U S A. 1965 Jun;53(6):1430–1436. doi: 10.1073/pnas.53.6.1430. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Setlow J. K., Brown D. C., Boling M. E., Mattingly A., Gordon M. P. Repair of deoxyribonucleic acid in Haemophilus influenzae. I. X-ray sensitivity of ultraviolet-sensitive mutants and their behavior as hosts to ultraviolet-irradiated bacteriophage and transforming deoxyribonucleic acid. J Bacteriol. 1968 Feb;95(2):546–558. doi: 10.1128/jb.95.2.546-558.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Setlow J. K., Randolph M. L., Boling M. E., Mattingly A., Price G., Gordon M. P. Repair of DNA in Haemophilus influenzae. II. Excision, repair of single-strand breaks, defects in transformation, and host cell modification in UV-sensitive mutants. Cold Spring Harb Symp Quant Biol. 1968;33:209–218. doi: 10.1101/sqb.1968.033.01.024. [DOI] [PubMed] [Google Scholar]
  9. Setlow R. B., Carrier W. L. Pyrimidine dimers in ultraviolet-irradiated DNA's. J Mol Biol. 1966 May;17(1):237–254. doi: 10.1016/s0022-2836(66)80105-5. [DOI] [PubMed] [Google Scholar]
  10. Spencer H. T., Herriott R. M. Development of competence of Haemophilus influenzae. J Bacteriol. 1965 Oct;90(4):911–920. doi: 10.1128/jb.90.4.911-920.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Steinhart W. L., Herriott R. M. Fate of recipient deoxyribonucleic acid during transformation in Haemophilus influenzae. J Bacteriol. 1968 Nov;96(5):1718–1724. doi: 10.1128/jb.96.5.1718-1724.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Steinhart W. L., Herriott R. M. Genetic integration in the heterospecific transformation of Haemophilus influenzae cells by Haemophilus parainfluenzae deoxyribonucleic acid. J Bacteriol. 1968 Nov;96(5):1725–1731. doi: 10.1128/jb.96.5.1725-1731.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES