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. 1970 Mar;101(3):965–972. doi: 10.1128/jb.101.3.965-972.1970

Depression of Guanosine Diphosphate-Mannose Pyrophosphorylase by Mutations in Two Different Regulator Genes Involved in Capsular Polysaccharide Synthesis in Escherichia coli K-12

Michael M Lieberman a,1, Alvin Markovitz a
PMCID: PMC250417  PMID: 4908790

Abstract

Mutations in a regulator gene (capR) that causes increased synthesis of capsular polysaccharide and derepressed synthesis of several enzymes involved in polysaccharide synthesis also derepress synthesis of guanosine diphosphate (GDP)-mannose pyrophosphorylase. In addition, a second mucoid mutation (capS, which maps separately from capR) also results in the derepression of GDP-mannose pyrophosphorylase. New conditions for assaying GDP-mannose hydrolyase and GDP-l-fucose synthetase permitted us to show that these enzymes are also derepressed in the capS mucoid strain. Although phosphomannose isomerase and uridine diphosphate-galactose-4-epimerase are derepressed in capR mucoid strains, they are not derepressed in capS mucoid strains. A nonmucoid mutant of a strain containing the capR9 (mucoid) allele was deficient in GDP-mannose pyrophosphorylase.

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Selected References

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