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. 1972 Aug;111(2):404–411. doi: 10.1128/jb.111.2.404-411.1972

Nature of the Receptor Substance of Mycobacterium smegmatis for D4 Bacteriophage Adsorption

Atsuko Furuchi 1, Tohru Tokunaga 2
PMCID: PMC251297  PMID: 5053464

Abstract

The acetone-soluble fraction extracted from lyophilized cells of Mycobacterium smegmatis ATCC 607 inhibited D4, a species-specific phage active against M. smegmatis. Evidence is presented indicating that the D4 inhibition was caused by the phage receptor substance(s) contained in this fraction. A fraction eluted from silicic acid with chloroform-methanol (95:5, v/v) showed the strongest inhibition of D4 phage. This fraction contained sugars and amino acids, and its infrared absorption spectrum was practically identical with those of the mycoside C isolated from the other species of mycobacteria. Further fractionation revealed that the active material was a mixture of several closely related peptidoglycolipids all of which showed, more or less, the phage inhibition. One of the compounds was purified and partially characterized; it contains three different amino acids, allo-threonine, alanine, and phenylalanine, at a molar ratio of 1:1:1, and also three different deoxyhexoses, probably 6-deoxytalose, 3,4-di-o-methylrhamnose, and 2,3,4-tri-o-methylrhamnose. A tentative name of “mycoside Csm” is proposed for this substance which possesses a slightly different structure from the known types of mycoside C and is probably specific for the species of M. smegmatis. A fraction extracted from the D4-resistant mutant of M. smegmatis ATCC 607 by acetone and then by chloroform-methanol (95:5, v/v) showed no phage inhibition and had no sugar component. In addition, this fraction contained lysine, serine, and a small amount of both glycine and an unidentified amino acid.

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Selected References

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  1. BACON J. S. D., EDELMAN J. The carbohydrates of the Jerusalem artichoke and other Compositae. Biochem J. 1951 Jan;48(1):114–126. doi: 10.1042/bj0480114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CHAPUT M., MICHEL G., LEDERER E. STRUCTURE DU MYCOSIDE C2 DE MYCOBACTERIUM AVIUM. Biochim Biophys Acta. 1963 Oct 29;78:329–341. doi: 10.1016/0006-3002(63)91643-3. [DOI] [PubMed] [Google Scholar]
  3. CHAPUT M., MICHEL G., LEDERER E. [On the mycoside Cm, a new peptido-glycolipid isolated from Mycobacterium marianum]. Experientia. 1961 Mar 15;17:107–108. doi: 10.1007/BF02160810. [DOI] [PubMed] [Google Scholar]
  4. CHAPUT M., MICHEL G., LEDERER E. [Structure of mycoside Cm, peptidoglycolipid of Mycobacterium marianum]. Biochim Biophys Acta. 1962 Sep 24;63:310–326. doi: 10.1016/0006-3002(62)90685-6. [DOI] [PubMed] [Google Scholar]
  5. Castelnuovo G., Bellezza G., Yamanaka S. Phage inhibiting substances of mycobacteria. I. The nature of phage inhibiting substances of Mycobacterium phlei. Ann Inst Pasteur (Paris) 1970 Sep;119(3):302–311. [PubMed] [Google Scholar]
  6. DITTMER J. C., LESTER R. L. A SIMPLE, SPECIFIC SPRAY FOR THE DETECTION OF PHOSPHOLIPIDS ON THIN-LAYER CHROMATOGRAMS. J Lipid Res. 1964 Jan;5:126–127. [PubMed] [Google Scholar]
  7. Drozdz B., Kowalewski Z. Selective detection of various types of sugars present in cardiac heterosides. J Chromatogr. 1965 Oct;20(1):188–188. doi: 10.1016/s0021-9673(01)97394-7. [DOI] [PubMed] [Google Scholar]
  8. FROMAN S., WILL D. W., BOGEN E. Bacteriophage active against virulent Mycobacterium tuberculosis. I. Isolation and activity. Am J Public Health Nations Health. 1954 Oct;44(10):1326–1333. doi: 10.2105/ajph.44.10.1326. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Glaser L., Ionesco H., Schaeffer P. Teichoic acids as components of a specific phage receptor in Bacillus subtilis. Biochim Biophys Acta. 1966 Aug 24;124(2):415–417. doi: 10.1016/0304-4165(66)90211-x. [DOI] [PubMed] [Google Scholar]
  10. IKAWA M., SNELL E. E., LEDERER E. Occurrence of D-phenylalanine, D-allothreonine and other D-amino-acids in peptido-lipids of bacterial origin. Nature. 1960 Nov 12;188:558–560. doi: 10.1038/188558a0. [DOI] [PubMed] [Google Scholar]
  11. JOLLES P., BIGLER F., GENDRE T., LEDERER E. [On the chemical structure of "myoside C", a peptide-glycolipid from Mycobacterium avium]. Bull Soc Chim Biol (Paris) 1961;43:177–192. [PubMed] [Google Scholar]
  12. Lanéelle M. A., Lanéelle G., Bennet P., Asselineau J. Sur les lipides d'une souche non-photochromogène de mycobactérie. Bull Soc Chim Biol (Paris) 1965;47(11):2047–2067. [PubMed] [Google Scholar]
  13. Lindberg A. A., Hellerqvist C. G. Bacteriophage attachment sites, serological specificity, and chemical composition of the lipopolysaccharides of semirough and rough mutants of Salmonella typhimurium. J Bacteriol. 1971 Jan;105(1):57–64. doi: 10.1128/jb.105.1.57-64.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. MACLENNAN A. P. The monosaccharide units in specific glycolipids of Mycobacterium avium. Biochem J. 1962 Mar;82:394–400. doi: 10.1042/bj0820394. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Michael J. G. The surface antigens and phage receptors in Escherichia coli B. Proc Soc Exp Biol Med. 1968 Jun;128(2):434–438. doi: 10.3181/00379727-128-33031. [DOI] [PubMed] [Google Scholar]
  16. ROE J. H. The determination of sugar in blood and spinal fluid with anthrone reagent. J Biol Chem. 1955 Jan;212(1):335–343. [PubMed] [Google Scholar]
  17. SELLERS M. I., BAXTER W. L., RUNNALS H. R. Growth characteristics of mycobacteriophages D28 and D29. Can J Microbiol. 1962 Jun;8:389–399. doi: 10.1139/m62-051. [DOI] [PubMed] [Google Scholar]
  18. SELLES M. I., RUNNALS H. R. Mycobacteriophage. I. Physicochemical characterization. J Bacteriol. 1961 Mar;81:442–447. doi: 10.1128/jb.81.3.442-447.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. SMITH D. W., RANDALL H. M., MACLENNAN A. P., LEDERER E. Mycosides: a new class of type-specific glycolipids of Mycobacteria. Nature. 1960 Jun 11;186:887–888. doi: 10.1038/186887a0. [DOI] [PubMed] [Google Scholar]
  20. SMITH D. W., RANDALL H. M., MACLENNAN A. P., PUTNEY R. K., RAO S. V. Detection of specific lipids in mycobacteria by infrared spectroscopy. J Bacteriol. 1960 Feb;79:217–229. doi: 10.1128/jb.79.2.217-229.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. TOKUNAGA T., SELLERS M. INFECTION OF MYCOBACTERIUM SMEGMATIS WITH D29 PHAGE DNA. J Exp Med. 1964 Jan 1;119:139–149. doi: 10.1084/jem.119.1.139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Tokunaga T., Kataoka T., Suga K. Phage inactivation by an ethanol-ether extract of Mycobacterium smegmatis. Am Rev Respir Dis. 1970 Feb;101(2):309–313. doi: 10.1164/arrd.1970.101.2.309. [DOI] [PubMed] [Google Scholar]
  23. Tokunaga T., Kataoka T., Suga K. [Bacteriophage receptor of Mycobacteria. 1. Preliminary experiments on the phage-receptor of Mycobacterium smegmatis]. Igaku To Seibutsugaku. 1968 Dec 10;77(6):225–229. [PubMed] [Google Scholar]
  24. Tokunaga T., Maruyama Y., Murohashi T. [Further studies on the phage typing of rapidly growing Mycobacteria]. Nihon Saikingaku Zasshi. 1965 Sep;20(9):554–559. doi: 10.3412/jsb.20.554. [DOI] [PubMed] [Google Scholar]
  25. VILKAS E., MIQUEL A. M., LEDERER E. [On the isolation and structure of fortuitine, peptidolipid from Mycobacterium fortuitum]. Biochim Biophys Acta. 1963 Apr 23;70:217–218. doi: 10.1016/0006-3002(63)90745-5. [DOI] [PubMed] [Google Scholar]
  26. Vidaver A. K., Brock T. D. Purification and properties of a bacteriophage receptor material from Streptococcus faecium. Biochim Biophys Acta. 1966 Jun 29;121(2):298–314. doi: 10.1016/0304-4165(66)90119-x. [DOI] [PubMed] [Google Scholar]
  27. WEIDEL W. Bacterial viruses; with particular reference to adsorption/penetration. Annu Rev Microbiol. 1958;12:27–48. doi: 10.1146/annurev.mi.12.100158.000331. [DOI] [PubMed] [Google Scholar]
  28. WHITE A., KNIGHT V. Effect of tween 80 and serum on the interaction of mycobacteriophage D-29 with certain mycobacterial species. Am Rev Tuberc. 1958 Jan;77(1):134–145. doi: 10.1164/artpd.1958.77.1.134. [DOI] [PubMed] [Google Scholar]

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