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. 1972 Aug;111(2):443–446. doi: 10.1128/jb.111.2.443-446.1972

Cell Wall Alterations Associated with the Hyperproduction of Extracellular Enzymes in Neurospora crassa

Howard G Gratzner 1
PMCID: PMC251302  PMID: 4262302

Abstract

A pleiotropic mutation in Neurospora (exo-1), which confers derepression of α-amylase, glucoamylase, β-fructofuranosidase, and trehalase, appears to also affect the composition of the cell wall. Segregants resulting from the backcross of exo-1 to the wild-type strain from which it derived are altered in the ratio of galactosamine to glucosamine in hydrolysates of isolated cell walls. Conidial cell walls exhibit a marked decrease in the amount of galactosamine in both exo-1 and exo-1+ strains. Increased levels (approximately sevenfold) of amylase are found in conidia of exo-1, as compared with those of exo-1+.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brody S., Tatum E. L. The primary biochemical effect of a morphological mutation in Neurospora crassa. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1290–1297. doi: 10.1073/pnas.56.4.1290. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chung P. L., Trevithick J. R. Biochemical and histochemical localization of invertase in Neurospora crassa during conidial germination and hyphal growth. J Bacteriol. 1970 May;102(2):423–429. doi: 10.1128/jb.102.2.423-429.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gratzner H., Sheehan D. N. Neurospora mutant exhibiting hyperproduction of amylase and invertase. J Bacteriol. 1969 Feb;97(2):544–549. doi: 10.1128/jb.97.2.544-549.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Lee Y. C., Scocca J. R., Muir L. Rapid automatic analysis of sugar components in glycoproteins. I. Hexosamines. Anal Biochem. 1969 Mar;27(3):559–566. doi: 10.1016/0003-2697(69)90070-0. [DOI] [PubMed] [Google Scholar]
  5. Livingston L. R. Locus-specific changes in cell wall composition characteristic of osmotic mutants of Neurospora crassa. J Bacteriol. 1969 Jul;99(1):85–90. doi: 10.1128/jb.99.1.85-90.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Mahadevan P. R., Mahadkar U. R. Major constituents of the conidial wall of Neurospora crassa. Indian J Exp Biol. 1970 Jul;8(3):207–210. [PubMed] [Google Scholar]
  7. Mahadevan P. R., Tatum E. L. Relationship of the major constituents of the Neurospora crassa cell wall to wild-type and colonial morphology. J Bacteriol. 1965 Oct;90(4):1073–1081. doi: 10.1128/jb.90.4.1073-1081.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Yabuki M., Fukui S. Presence of binding site for alpha-amylase and of masking protein for this site on mycelial cell wall of Aspergillus oryzae. J Bacteriol. 1970 Oct;104(1):138–144. doi: 10.1128/jb.104.1.138-144.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]

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