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. 1972 Sep;111(3):641–648. doi: 10.1128/jb.111.3.641-648.1972

Allostery of 3-Deoxy-d-arabino-Heptulosonate 7-Phosphate Synthetase in Clostridium: Another Conserved Generic Characteristic

Roy A Jensen 1, Robert Twarog 2
PMCID: PMC251335  PMID: 5053880

Abstract

Enzymological studies were done to characterize the allosteric control of 3-deoxy-d-arabino-heptulosonate 7-phosphate (DAHP) synthetase in three species of Clostridium. Allosteric control was identified as feedback inhibition by phenylalanine and was qualitatively similar for the DAHP synthetases of C. butyricum, C. acetobutylicum, and C. tetanomorphum. Quantitative differences in the enzymology and kinetics of allosteric control distinguished C. tetanomorphum from C. butyricum and C. acetobutylicum. Crude extracts contained apparent proteolytic activity which could be fractionated from DAHP synthetase. The proteolytic activity was more labile than DAHP synthetase in extracts and was progressively inactivated by serial freeze-thaw treatments. Protease activity was at least partially inhibited by phenylmethylsulfonyl-fluoride. The method of comparative allostery of DAHP synthetase distinguishes the genera Bacillus and Clostridium, each having a strongly conserved pattern of regulation for DAHP synthetase. The data reinforce previous conclusions that allosteric control patterns governing the activity of DAHP synthetase are stable, reliable generic characteristics.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. COHEN-BAZIRE G., SISTROM W. R., STANIER R. Y. Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Physiol. 1957 Feb;49(1):25–68. doi: 10.1002/jcp.1030490104. [DOI] [PubMed] [Google Scholar]
  2. De Ley J. Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. J Bacteriol. 1970 Mar;101(3):738–754. doi: 10.1128/jb.101.3.738-754.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Foubert E. L., Douglas H. C. Studies on the Anaerobic Micrococci: II. The Fermentation of Lactate by Micrococcus lactilyticus. J Bacteriol. 1948 Jul;56(1):35–36. [PMC free article] [PubMed] [Google Scholar]
  4. Hill L. R. An index to deoxyribonucleic acid base compositions of bacterial species. J Gen Microbiol. 1966 Sep;44(3):419–437. doi: 10.1099/00221287-44-3-419. [DOI] [PubMed] [Google Scholar]
  5. JENSEN R. A., NESTER E. W. THE REGULATORY SIGNIFICANCE OF INTERMEDIARY METABOLITES: CONTROL OF AROMATIC ACID BIOSYNTHESIS BY FEEDBACK INHIBITION IN BACILLUS SUBTILIS. J Mol Biol. 1965 Jun;12:468–481. doi: 10.1016/s0022-2836(65)80270-4. [DOI] [PubMed] [Google Scholar]
  6. Jensen R. A., Nasser D. S., Nester E. W. Comparative control of a branch-point enzyme in microorganisms. J Bacteriol. 1967 Nov;94(5):1582–1593. doi: 10.1128/jb.94.5.1582-1593.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jensen R. A., Nester E. W. Regulatory enzymes of aromatic amino acid biosynthesis in Bacillus subtilis. I. Purification and properties of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthetase. J Biol Chem. 1966 Jul 25;241(14):3365–3372. [PubMed] [Google Scholar]
  8. Jensen R. A., Stenmark S. L. Comparative allostery of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthetase as a molecular basis for classification. J Bacteriol. 1970 Mar;101(3):763–769. doi: 10.1128/jb.101.3.763-769.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jensen R. A. Taxonomic implications of temperature dependence of the allosteric inhibition of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthetase in Bacillus. J Bacteriol. 1970 May;102(2):489–497. doi: 10.1128/jb.102.2.489-497.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Mead G. C. The amino acid-fermenting clostridia. J Gen Microbiol. 1971 Jul;67(1):47–56. doi: 10.1099/00221287-67-1-47. [DOI] [PubMed] [Google Scholar]
  12. Nester E. W., Jensen R. A. Control of aromatic acid biosynthesis in Bacillus subtilis: sequenial feedback inhibition. J Bacteriol. 1966 Apr;91(4):1594–1598. doi: 10.1128/jb.91.4.1594-1598.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Richmond R. C. Non-Darwinian evolution: a critique. Nature. 1970 Mar 14;225(5237):1025–1028. doi: 10.1038/2251025a0. [DOI] [PubMed] [Google Scholar]

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