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. 1972 Sep;111(3):791–796. doi: 10.1128/jb.111.3.791-796.1972

Antibody-Mediated Modification of the Binding Properties of a Protein Related to Galactose Transport

Boris Rotman 1, John H Ellis Jr 1
PMCID: PMC251354  PMID: 4559829

Abstract

A galactose-binding protein related to the mglP transport system of Escherichia coli increases its affinity and binding capacity for the substrate when exposed to both d-galactose and specific antibodies. For this increase to occur, the binding protein has to be in contact with d-galactose for at least 2 min prior to the addition of the antibodies. This reaction was used to show that other substrates of the mglP transport system compete with galactose for a site(s) of the binding protein and that the degree of competition is comparable to that observed in vivo. A model for substrate translocation is presented postulating a cellular component that can induce conformational changes in the galactose-binding protein similar to those caused by antibodies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anraku Y. Transport of sugars and amino acids in bacteria. 3. Studies on the restoration of active transport. J Biol Chem. 1968 Jun 10;243(11):3128–3135. [PubMed] [Google Scholar]
  2. Anraku Y. Transport of sugars and amino acids in bacteria. I. Purification and specificity of the galactose- and leucine-binding proteins. J Biol Chem. 1968 Jun 10;243(11):3116–3122. [PubMed] [Google Scholar]
  3. Boos W., Gordon A. S., Hall R. E., Price H. D. Transport properties of the galactose-binding protein of Escherichia coli. Substrate-induced conformational change. J Biol Chem. 1972 Feb 10;247(3):917–924. [PubMed] [Google Scholar]
  4. Boos W., Sarvas M. O. Close linkage between a galactose binding protein and the beta-methylgalactoside permease in Escherichia coli. Eur J Biochem. 1970 Apr;13(3):526–533. doi: 10.1111/j.1432-1033.1970.tb00956.x. [DOI] [PubMed] [Google Scholar]
  5. Boos W. The galactose binding protein and its relationship to the beta-methylgalactoside permease from Escherichia coli. Eur J Biochem. 1969 Aug;10(1):66–73. doi: 10.1111/j.1432-1033.1969.tb00656.x. [DOI] [PubMed] [Google Scholar]
  6. Crumpton M. J. Conformational changes in sperm-whale metmyoglobin due to combination with antibodies to apomyoglobin. Biochem J. 1966 Jul;100(1):223–232. doi: 10.1042/bj1000223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ganesan A. K., Rotman B. Transport systems for galactose and galactosides in Escherichia coli. I. Genetic determination and regulation of the methyl-galactoside permease. J Mol Biol. 1966 Mar;16(1):42–50. doi: 10.1016/s0022-2836(66)80261-9. [DOI] [PubMed] [Google Scholar]
  8. Hazelbauer G. L., Adler J. Role of the galactose binding protein in chemotaxis of Escherichia coli toward galactose. Nat New Biol. 1971 Mar 24;230(12):101–104. doi: 10.1038/newbio230101a0. [DOI] [PubMed] [Google Scholar]
  9. Hogg R. W., Englesberg E. L-arabinose binding protein from Escherichia coli B-r. J Bacteriol. 1969 Oct;100(1):423–432. doi: 10.1128/jb.100.1.423-432.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kaback H. R. Transport. Annu Rev Biochem. 1970;39:561–598. doi: 10.1146/annurev.bi.39.070170.003021. [DOI] [PubMed] [Google Scholar]
  11. Kalckar H. M. The periplasmic galactose binding protein of Escherichia coli. Science. 1971 Nov 5;174(4009):557–565. doi: 10.1126/science.174.4009.557. [DOI] [PubMed] [Google Scholar]
  12. Lin E. C. The genetics of bacterial transport systems. Annu Rev Genet. 1970;4:225–262. doi: 10.1146/annurev.ge.04.120170.001301. [DOI] [PubMed] [Google Scholar]
  13. Neu H. C., Heppel L. A. The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts. J Biol Chem. 1965 Sep;240(9):3685–3692. [PubMed] [Google Scholar]
  14. POLLOCK M. R. STIMULATING AND INHIBITING ANTIBODIES FOR BACTERIAL PENICILLINASE. Immunology. 1964 Nov;7:707–723. [PMC free article] [PubMed] [Google Scholar]
  15. PORTER R. R. The hydrolysis of rabbit y-globulin and antibodies with crystalline papain. Biochem J. 1959 Sep;73:119–126. doi: 10.1042/bj0730119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pardee A. B. Membrane transport proteins. Proteins that appear to be parts of membrane transport systems are being isolated and characterized. Science. 1968 Nov 8;162(3854):632–637. doi: 10.1126/science.162.3854.632. [DOI] [PubMed] [Google Scholar]
  17. ROTMAN B., RADOJKOVIC J. GALACTOSE TRANSPORT IN ESCHERICHIA COLI. THE MECHANISM UNDERLYING THE RETENTION OF INTRACELLULAR GALACTOSE. J Biol Chem. 1964 Oct;239:3153–3156. [PubMed] [Google Scholar]
  18. Rotman B., Ganesan A. K., Guzman R. Transport systems for galactose and galactosides in Escherichia coli. II. Substrate and inducer specificities. J Mol Biol. 1968 Sep 14;36(2):247–260. doi: 10.1016/0022-2836(68)90379-3. [DOI] [PubMed] [Google Scholar]
  19. Rotman M. B., Celada F. Antibody-mediated activation of a defective beta-D-galactosidase extracted from an Escherichia coli mutant. Proc Natl Acad Sci U S A. 1968 Jun;60(2):660–667. doi: 10.1073/pnas.60.2.660. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Taylor A. L. Current linkage map of Escherichia coli. Bacteriol Rev. 1970 Jun;34(2):155–175. doi: 10.1128/br.34.2.155-175.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]

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