Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1972 Oct;112(1):1–6. doi: 10.1128/jb.112.1.1-6.1972

Analysis of Ribosomes from Viomycin-Sensitive and -Resistant Strains of Mycobacterium smegmatis

Takeshi Yamada 1, Kunitsugu Masuda 1, Ko Shoji 1, Mitsuo Hori 1
PMCID: PMC251373  PMID: 4342812

Abstract

Viomycin-resistant strains were isolated from Mycobacterium smegmatis. Ribosomes were isolated and tested for drug resistance in subcellular systems containing poly(U) as messenger ribonucleic acid. Resistance to viomycin in these strains was due to altered ribosomes. Further analysis showed that viomycin resistance of two mutants with low level resistance (20 μg/ml) was due to altered 30S ribosomal subunits. Another mutant that was highly resistant to viomycin (1 mg/ml), however, had altered 50S ribosomal subunits.

Full text

PDF
4

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARTZ Q. R., EHRLICH J., MOLD J. D., PENNER M. A., SMITH R. M. Viomycin, a new tuberculostatic antibiotic. Am Rev Tuberc. 1951 Jan;63(1):4–6. doi: 10.1164/art.1951.63.1.4. [DOI] [PubMed] [Google Scholar]
  2. Beljanski M., Bourgarel P., Beljanski M. Drastic alteration of ribosomal RNA and ribosomal proteins in showdomycin-resistant Escherichia coli. Proc Natl Acad Sci U S A. 1971 Feb;68(2):491–495. doi: 10.1073/pnas.68.2.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Benveniste R., Yamada T., Davies J. Enzymatic Adenylylation of Streptomycin and Spectinomycin by R-Factor-Resistant Escherichia coli. Infect Immun. 1970 Jan;1(1):109–119. doi: 10.1128/iai.1.1.109-119.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bollen A., Davies J., Ozaki M., Mizushima S. Ribosomal protein conferring sensitivity to the antibiotic spectinomycin in Escherichia coli. Science. 1968 Jul 4;165(3888):85–86. [PubMed] [Google Scholar]
  5. Bollen A., Helser T., Yamada T., Davies J. Altered ribosomes in antibiotic-resistant mutants of E. coli. Cold Spring Harb Symp Quant Biol. 1969;34:95–100. doi: 10.1101/sqb.1969.034.01.015. [DOI] [PubMed] [Google Scholar]
  6. COX E. C., WHITE J. R., FLAKS J. G. STREPTOMYCIN ACTION AND THE RIBOSOME. Proc Natl Acad Sci U S A. 1964 Apr;51:703–709. doi: 10.1073/pnas.51.4.703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Capecchi M. R. Polypeptide chain termination in vitro: isolation of a release factor. Proc Natl Acad Sci U S A. 1967 Sep;58(3):1144–1151. doi: 10.1073/pnas.58.3.1144. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. DAVIES J. E. STUDIES ON THE RIBOSOMES OF STREPTOMYCIN-SENSITIVE AND RESISTANT STRAINS OF ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1964 Apr;51:659–664. doi: 10.1073/pnas.51.4.659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Davies J., Anderson P., Davis B. D. Inhibition of protein synthesis by spectinomycin. Science. 1965 Sep 3;149(3688):1096–1098. doi: 10.1126/science.149.3688.1096. [DOI] [PubMed] [Google Scholar]
  10. Davies J., Gorini L., Davis B. D. Misreading of RNA codewords induced by aminoglycoside antibiotics. Mol Pharmacol. 1965 Jul;1(1):93–106. [PubMed] [Google Scholar]
  11. Krembel J., Apirion D. Changes in ribosomal proteins associated with mutants in a locus that affects Escherichia coli ribosomes. J Mol Biol. 1968 Apr 28;33(2):363–368. doi: 10.1016/0022-2836(68)90194-0. [DOI] [PubMed] [Google Scholar]
  12. Nomura M., Mizushima S., Ozaki M., Traub P., Lowry C. V. Structure and function of ribosomes and their molecular components. Cold Spring Harb Symp Quant Biol. 1969;34:49–61. doi: 10.1101/sqb.1969.034.01.009. [DOI] [PubMed] [Google Scholar]
  13. Okamoto S., Suzuki Y. Chloramphenicol-, dihydrostreptomycin-, and kanamycin-inactivating enzymes from multiple drug-resistant Escherichia coli carrying episome 'R'. Nature. 1965 Dec 25;208(5017):1301–1303. doi: 10.1038/2081301a0. [DOI] [PubMed] [Google Scholar]
  14. Ozaki M., Mizushima S., Nomura M. Identification and functional characterization of the protein controlled by the streptomycin-resistant locus in E. coli. Nature. 1969 Apr 26;222(5191):333–339. doi: 10.1038/222333a0. [DOI] [PubMed] [Google Scholar]
  15. Tanaka K., Teraoka H., Tamaki M., Otaka E., Osawa S. Erythromycin-resistant mutant of Escherichia coli with altered ribosomal protein component. Science. 1968 Nov 1;162(3853):576–578. doi: 10.1126/science.162.3853.576. [DOI] [PubMed] [Google Scholar]
  16. Tanaka N., Igusa S. Effects of viomycin and polymyxin B on protein synthesis in vitro. J Antibiot (Tokyo) 1968 Mar;21(3):239–240. doi: 10.7164/antibiotics.21.239. [DOI] [PubMed] [Google Scholar]
  17. Umezawa H., Takasawa S., Okanishi M., Utahara R. Adenylylstreptomycin, a product of streptomycin inactivated by E. coli carrying R factor. J Antibiot (Tokyo) 1968 Jan;21(1):81–82. doi: 10.7164/antibiotics.21.81. [DOI] [PubMed] [Google Scholar]
  18. Weisblum B., Davies J. Antibiotic inhibitors of the bacterial ribosome. Bacteriol Rev. 1968 Dec;32(4 Pt 2):493–528. [PMC free article] [PubMed] [Google Scholar]
  19. Yamada T., Tipper D., Davies J. Enzymatic inactivation of streptomycin by R factor-resistant Escherichia coli. Nature. 1968 Jul 20;219(5151):288–291. doi: 10.1038/219288a0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES