Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1972 Oct;112(1):7–12. doi: 10.1128/jb.112.1.7-12.1972

Initiation of Deoxyribonucleic Acid Replication in Escherichia coli B/r: Chronology of Events and Transcriptional Control of Initiation

Walter Messer 1
PMCID: PMC251374  PMID: 4562418

Abstract

Three processes necessary for the initiation of deoxyribonucleic acid replication have been separated in time in synchronously growing Escherichia coli B/r. One can be inhibited with 2 μg of chloramphenicol per ml and occurs about 20 min prior to initiation. A second step, occurring 5 to 10 min prior to initiation, is sensitive to 4 to 30 μg of chloramphenicol per ml. A third process occurs at the time of initiation and can be inhibited with rifampin. The experiments suggest that transcription itself, or the synthesis of a relatively small ribonucleic acid, is required for initiation of replication.

Full text

PDF
9

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brutlag D., Schekman R., Kornberg A. A possible role for RNA polymerase in the initiation of M13 DNA synthesis. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2826–2829. doi: 10.1073/pnas.68.11.2826. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Clark D. J. The regulation of DNA replication and cell division in E. coli B-r. Cold Spring Harb Symp Quant Biol. 1968;33:823–838. doi: 10.1101/sqb.1968.033.01.094. [DOI] [PubMed] [Google Scholar]
  3. Cooper S., Weusthoff G. Comment on the use of chloramphenicol to study the initiation of deoxyribonucleic acid synthesis. J Bacteriol. 1971 May;106(2):709–711. doi: 10.1128/jb.106.2.709-711.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. HELMSTETTER C. E., CUMMINGS D. J. AN IMPROVED METHOD FOR THE SELECTION OF BACTERIAL CELLS AT DIVISION. Biochim Biophys Acta. 1964 Mar 16;82:608–610. doi: 10.1016/0304-4165(64)90453-2. [DOI] [PubMed] [Google Scholar]
  5. Helmstetter C. E., Pierucci O. Cell division during inhibition of deoxyribonucleic acid synthesis in Escherichia coli. J Bacteriol. 1968 May;95(5):1627–1633. doi: 10.1128/jb.95.5.1627-1633.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Helmstetter C., Cooper S., Pierucci O., Revelas E. On the bacterial life sequence. Cold Spring Harb Symp Quant Biol. 1968;33:809–822. doi: 10.1101/sqb.1968.033.01.093. [DOI] [PubMed] [Google Scholar]
  7. LARK C., LARK K. G. EVIDENCE FOR TWO DISTINCT ASPECTS OF THE MECHANISM REGULATING CHROMOSOME REPLICATION IN ESCHERICHIA COLI. J Mol Biol. 1964 Oct;10:120–136. doi: 10.1016/s0022-2836(64)80032-2. [DOI] [PubMed] [Google Scholar]
  8. LARK K. G., REPKO T., HOFFMAN E. J. THE EFFECT OF AMINO ACID DEPRIVATION ON SUBSEQUENT DEOXYRIBONUCLEIC ACID REPLICATION. Biochim Biophys Acta. 1963 Sep 17;76:9–24. [PubMed] [Google Scholar]
  9. Lark K. G., Lark C. Regulation of chromosome replication in Escherichia coli: a comparison of the effects of phenethyl alcohol treatment with those of amino acid starvation. J Mol Biol. 1966 Sep;20(1):9–19. doi: 10.1016/0022-2836(66)90113-6. [DOI] [PubMed] [Google Scholar]
  10. Lark K. G., Renger H. Initiation of DNA replication in Escherichia coli 15T-: chronological dissection of three physiological processes required for initiation. J Mol Biol. 1969 Jun 14;42(2):221–235. doi: 10.1016/0022-2836(69)90039-4. [DOI] [PubMed] [Google Scholar]
  11. MAALOE O., HANAWALT P. C. Thymine deficiency and the normal DNA replication cycle. I. J Mol Biol. 1961 Apr;3:144–155. doi: 10.1016/s0022-2836(61)80041-7. [DOI] [PubMed] [Google Scholar]
  12. Mosteller R. D., Yanofsky C. Transcription of the tryptophan operon in Escherichia coli: rifampicin as an inhibitor of initiation. J Mol Biol. 1970 Mar;48(3):525–531. doi: 10.1016/0022-2836(70)90064-1. [DOI] [PubMed] [Google Scholar]
  13. Rose J. K., Mosteller R. D., Yanofsky C. Tryptophan messenger ribonucleic acid elongation rates and steady-state levels of tryptophan operon enzymes under various growth conditions. J Mol Biol. 1970 Aug;51(3):541–550. doi: 10.1016/0022-2836(70)90007-0. [DOI] [PubMed] [Google Scholar]
  14. Sekiguchi M., Iida S. Mutants of Escherichia coli permeable to actinomycin. Proc Natl Acad Sci U S A. 1967 Dec;58(6):2315–2320. doi: 10.1073/pnas.58.6.2315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Silverstein S., Billen D. Transcription: role in the initiation and replication of DNA synthesis in Escherichia coli and phiX174. Biochim Biophys Acta. 1971 Oct;247(3):383–390. doi: 10.1016/0005-2787(71)90023-2. [DOI] [PubMed] [Google Scholar]
  16. Ward C. B., Glaser D. A. Analysis of the chloramphenicol-sensitive and chloramphenicol-resistant steps in the initiation of DNA synthesis in E. coli B-r. Proc Natl Acad Sci U S A. 1969 Nov;64(3):905–912. doi: 10.1073/pnas.64.3.905. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wickner W., Brutlag D., Schekman R., Kornberg A. RNA synthesis initiates in vitro conversion of M13 DNA to its replicative form. Proc Natl Acad Sci U S A. 1972 Apr;69(4):965–969. doi: 10.1073/pnas.69.4.965. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES