Abstract
The transforming activity of Bacillus subtilis deoxyribonucleic acid (DNA) that had been sheared and purified with respect to size by sucrose gradient sedimentation is given as a function of the DNA molecular weight. It is shown (i) that fragments of median molecular weight 1.2 × 106 have finite activity (10−4), (ii) that the shape of the activity-versus-molecular weight function is qualitatively similar to that observed previously for Diplococcus pneumoniae, and (iii) that this shape precludes interpretation in terms of critical size models.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cato A., Jr, Guild W. R. Transformation and DNA size. I. Activity of fragments of defined size and a fit to a random double cross-over model. J Mol Biol. 1968 Oct 14;37(1):157–178. doi: 10.1016/0022-2836(68)90080-6. [DOI] [PubMed] [Google Scholar]
- Guild W. R., Cato A., Jr, Lacks S. Transformation and DNA size: two controlling parameters and the efficiency of the single strand intermediate. Cold Spring Harb Symp Quant Biol. 1968;33:643–645. doi: 10.1101/sqb.1968.033.01.072. [DOI] [PubMed] [Google Scholar]
- Lacks S. Theoretical relationship between probability of marker integration and length of donor DNA in pneumococcal transformation. J Mol Biol. 1968 Oct 14;37(1):179–180. doi: 10.1016/0022-2836(68)90081-8. [DOI] [PubMed] [Google Scholar]
- Morrison D. A. Early intermediate state of transforming deoxyribonucleic acid during uptake by Bacillus subtilis. J Bacteriol. 1971 Oct;108(1):38–44. doi: 10.1128/jb.108.1.38-44.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STUDIER F. W. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA. J Mol Biol. 1965 Feb;11:373–390. doi: 10.1016/s0022-2836(65)80064-x. [DOI] [PubMed] [Google Scholar]