Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1972 Nov;112(2):806–814. doi: 10.1128/jb.112.2.806-814.1972

Relationship Between Sporulation-Specific 20S Ribonucleic Acid and Ribosomal Ribonucleic Acid Processing in Saccharomyces cerevisiae

Stephen J Sogin 1, James E Haber 1, Harlyn O Halvorson 1
PMCID: PMC251490  PMID: 4563977

Abstract

The nature and properties of the 20S ribonucleic acid which accumulates only during the sporulation of Saccharomyces cerevisiae were examined. The 20S ribonucleic acid (RNA) has a base composition considerably different from ribosomal RNA species and is virtually unmethylated. The 20S RNA did, however, exhibit approximately 70% homology with 18S RNA by RNA-deoxyribonucleic acid filter hybridization competitions. The 20S RNA showed a hybridization saturation plateau level 30 to 40% higher than 18S, consistent with measurements of the size difference in polyacrylamide gels. Pulse-chase experiments in the presence and absence of cycloheximide indicate that the 20S RNA has a presumptive relationship to the 20S ribosomal RNA precursor normally observed only in short pulse-labeling in vegetative cells.

Full text

PDF
808

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bonner J., Kung G., Bekhor I. A method for the hybridization of nucleic acid molecules at low temperature. Biochemistry. 1967 Dec;6(12):3650–3653. doi: 10.1021/bi00864a005. [DOI] [PubMed] [Google Scholar]
  2. De Klt S. R. The formation of ribonucleic acid in yeast: hybridization of high molecular weight RNA species to yeast DNA. Arch Biochem Biophys. 1970 Feb;136(2):402–412. doi: 10.1016/0003-9861(70)90211-0. [DOI] [PubMed] [Google Scholar]
  3. Esposito M. S., Esposito R. E., Arnaud M., Halvorson H. O. Acetate utilization and macromolecular synthesis during sporulation of yeast. J Bacteriol. 1969 Oct;100(1):180–186. doi: 10.1128/jb.100.1.180-186.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gillespie D., Spiegelman S. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol. 1965 Jul;12(3):829–842. doi: 10.1016/s0022-2836(65)80331-x. [DOI] [PubMed] [Google Scholar]
  5. Kadowaki K., Halvorson H. O. Appearance of a new species of ribonucleic acid during sporulation in Saccharomyces cerevisiae. J Bacteriol. 1971 Mar;105(3):826–830. doi: 10.1128/jb.105.3.826-830.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kadowaki K., Halvorson H. O. Isolation and properties of a new species of ribonucleic acid synthesized in sporulating cells of Saccharomyces cerevisiae. J Bacteriol. 1971 Mar;105(3):831–836. doi: 10.1128/jb.105.3.831-836.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Loening U. E. The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochem J. 1967 Jan;102(1):251–257. doi: 10.1042/bj1020251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. MUNDKUR B. Electron microscopical studies of frozendried yeast. III. Formation of the tetrad in Saccharomyces. Exp Cell Res. 1961 Oct;25:24–40. doi: 10.1016/0014-4827(61)90304-4. [DOI] [PubMed] [Google Scholar]
  9. Maden B. E., Salim M., Summers D. F. Maturation pathway for ribosomal RNA in the Hela cell nucleolus. Nat New Biol. 1972 May 3;237(70):5–9. doi: 10.1038/newbio237005a0. [DOI] [PubMed] [Google Scholar]
  10. Retèl J., Planta R. J. The investigation of the ribosomal RNA sites in yeast DNA by the hybridization technique. Biochim Biophys Acta. 1968 Dec 17;169(2):416–429. doi: 10.1016/0005-2787(68)90050-6. [DOI] [PubMed] [Google Scholar]
  11. STAHL A. J., BAKES J., WEIL J. H., EBEL J. P. ETUDE DU TRANSFERT DU PHOSPHORE DES POLYPHOSPHATES INORGANIQUES DANS LES ACIDES RIBONUCH'EIQUES CHEZ LA LEVURE. II. DISTRIBUTION DU PHOSPHORE POLYPHOSPHORIQUE DANS LES DIVERSES FRACTIONS RIBONUCL'EIQUES. Bull Soc Chim Biol (Paris) 1964;46:1017–1026. [PubMed] [Google Scholar]
  12. Sanger F., Brownlee G. G., Barrell B. G. A two-dimensional fractionation procedure for radioactive nucleotides. J Mol Biol. 1965 Sep;13(2):373–398. doi: 10.1016/s0022-2836(65)80104-8. [DOI] [PubMed] [Google Scholar]
  13. Schweizer E., MacKechnie C., Halvorson H. O. The redundancy of ribosomal and transfer RNA genes in Saccharomyces cerevisiae. J Mol Biol. 1969 Mar 14;40(2):261–277. doi: 10.1016/0022-2836(69)90474-4. [DOI] [PubMed] [Google Scholar]
  14. Udem S. A., Warner J. R. Ribosomal RNA synthesis in Saccharomyces cerevisiae. J Mol Biol. 1972 Mar 28;65(2):227–242. doi: 10.1016/0022-2836(72)90279-3. [DOI] [PubMed] [Google Scholar]
  15. Vaughan M. H., Jr, Soeiro R., Warner J. R., Darnell J. E., Jr The effects of methionine deprivation on ribosome synthesis in HeLa cells. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1527–1534. doi: 10.1073/pnas.58.4.1527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. van den Bos R. C., Planta R. J. Studies on the role of rapidly labeled 20-S RNA in the biosynthesis of ribosomal RNA in yeast. Biochim Biophys Acta. 1971 Sep 30;247(1):175–180. doi: 10.1016/0005-2787(71)90822-7. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES