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. 1972 Dec;112(3):1193–1200. doi: 10.1128/jb.112.3.1193-1200.1972

Lipids of Thermoplasma acidophilum

Thomas A Langworthy 1, Paul F Smith 1, William R Mayberry 1
PMCID: PMC251548  PMID: 4344918

Abstract

Cells of Thermoplasma acidophilum contain about 3% total lipid on a dry weight basis. Total lipid was found to contain 17.5% neutral lipid, 25.1% glycolipid, and 56.6% phospholipid by chromatography on silicic acid. The lipids contain almost no fatty acid ester groups but appear to have long-chain alkyl groups in ether linkages to glycerol. The phospholipid fraction includes a major component which represents about 80% of the lipid phosphorus and 46% of the total lipids. We believe this component to be a long-chain isopranol glycerol diether analogue of glycerolphosphoryl monoglycosyl diglyceride. The glycolipids appear to contain isopranol diether analogues. Several components of the complex, neutral lipid fraction have been identified as hydrocarbons, vitamin K2-7, and isopranol glycerol diether analogues. Sterols are present in the neutral lipids but do not appear to be synthesized by the organism.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Carter H. E., Gaver R. C. Improved reagent for trimethylsilylation of sphingolipid bases. J Lipid Res. 1967 Jul;8(4):391–395. [PubMed] [Google Scholar]
  2. Darland G., Brock T. D., Samsonoff W., Conti S. F. A thermophilic, acidophilic mycoplasma isolated from a coal refuse pile. Science. 1970 Dec 25;170(3965):1416–1418. doi: 10.1126/science.170.3965.1416. [DOI] [PubMed] [Google Scholar]
  3. Gray G. M. A comparison of the glycolipids found in different strains of ascites tumour cells in mice. Nature. 1965 Jul 31;207(996):505–507. doi: 10.1038/207505a0. [DOI] [PubMed] [Google Scholar]
  4. HANES C. S., ISHERWOOD F. A. Separation of the phosphoric esters on the filter paper chromatogram. Nature. 1949 Dec 31;164(4183):1107-12, illust. doi: 10.1038/1641107a0. [DOI] [PubMed] [Google Scholar]
  5. Jonah M., Erwin J. A. The lipids of membraneous cell organelles isolated from the ciliate, Tetrahymena pyriformis. Biochim Biophys Acta. 1971 Feb 2;231(1):80–92. doi: 10.1016/0005-2760(71)90256-6. [DOI] [PubMed] [Google Scholar]
  6. KATES M., YENGOYAN L. S., SASTRY P. S. A DIETHER ANALOG OF PHOSPHATIDYL GLYCEROPHOSPHATE IN HALOBACTERIUM CUTIRUBRUM. Biochim Biophys Acta. 1965 Apr 5;98:252–268. doi: 10.1016/0005-2760(65)90119-0. [DOI] [PubMed] [Google Scholar]
  7. Kates M., Park C. E., Palameta B., Joo C. N. Synthesis of diphytanyl ether analogues of phosphatidic acid and cytidine diphosphate diglyceride. Can J Biochem. 1971 Mar;49(3):275–281. doi: 10.1139/o71-040. [DOI] [PubMed] [Google Scholar]
  8. LESTER R. L., WHITE D. C., SMITH S. L. THE 2-DESMETHYL VITAMIN K2'S. A NEW GROUP OF NAPHTHOQUINONES ISOLATED FROM HEMOPHILUS PARAINFLUENZAE. Biochemistry. 1964 Jul;3:949–954. doi: 10.1021/bi00895a018. [DOI] [PubMed] [Google Scholar]
  9. Plackett P., Smith P. F., Mayberry W. R. Lipids of a sterol-nonrequiring Mycoplasma. J Bacteriol. 1970 Nov;104(2):798–807. doi: 10.1128/jb.104.2.798-807.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ramasarma T. Lipid quinones. Adv Lipid Res. 1968;6:107–180. doi: 10.1016/b978-1-4831-9942-9.50010-3. [DOI] [PubMed] [Google Scholar]
  11. Shaw N., Smith P. F., Koostra W. L. The lipid composition of Mycoplasma laidlawii strain B. Biochem J. 1968 Apr;107(3):329–333. doi: 10.1042/bj1070329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Vaskovsky V. E., Kostetsky E. Y. Modified spray for the detection of phospholipids on thin-layer chromatograms. J Lipid Res. 1968 May;9(3):396–396. [PubMed] [Google Scholar]
  13. WELLS M. A., DITTMER J. C. THE USE OF SEPHADEX FOR THE REMOVAL OF NONLIPID CONTAMINANTS FROM LIPID EXTRACTS. Biochemistry. 1963 Nov-Dec;2:1259–1263. doi: 10.1021/bi00906a015. [DOI] [PubMed] [Google Scholar]
  14. White D. C., Frerman F. E. Extraction, characterization, and cellular localization of the lipids of Staphylococcus aureus. J Bacteriol. 1967 Dec;94(6):1854–1867. doi: 10.1128/jb.94.6.1854-1867.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]

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