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. 1973 Apr;114(1):53–58. doi: 10.1128/jb.114.1.53-58.1973

Sodium and Potassium Requirements for Active Transport of Glutamate by Escherichia coli K-12

Y S Halpern 1, H Barash 1, S Dover 1, Karnina Druck 1
PMCID: PMC251739  PMID: 4572725

Abstract

Active transport of glutamate by Escherichia coli K-12 requires both Na+ and K+ ions. Increasing the concentration of Na+ in the medium results in a decrease in the Km of the uptake system for glutamate; the capacity is not affected. Glutamate uptake by untreated cells is not stimulated by K+. K+-depleted cells show a greatly reduced capacity for glutamate uptake. Preincubation of such cells in the presence of K+ fully restores their capacity for glutamate uptake when Na+ ions are also present in the uptake medium. Addition of either K+ or Na+ alone restores glutamate uptake to only about 20% of its maximum capacity in the presence of both cations. Changes in K+ concentration affect the capacity for glutamate uptake but have no effect on the Km of the glutamate transport system. Ouabain does not inhibit the (Na+-K+)-stimulated glutamate uptake by intact cells or spheroplasts of E. coli K-12.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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