Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1973 May;114(2):549–555. doi: 10.1128/jb.114.2.549-555.1973

Osmotic Pressure in Escherichia coli as Rendered Detectable by Lysozyme Attack

Paul Scheie 1
PMCID: PMC251808  PMID: 4574692

Abstract

The enhanced susceptibility of plasmolyzed Escherichia coli to lysozyme attack was used to estimate the internal osmotic pressure of these cells under various conditions. Differences were detected between strains, culture media, stages in the growth cycle, and the osmotically active material used to produce plasmolysis. Lysozyme also was found to attack unplasmolyzed cells at 0 C and between 50 and 70 C.

Full text

PDF
552

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bayer M. E. Response of Cell Walls of Escherichia coli to a Sudden Reduction of the Environmental Osmotic Pressure. J Bacteriol. 1967 Mar;93(3):1104–1112. doi: 10.1128/jb.93.3.1104-1112.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Birdsell D. C., Cota-Robles E. H. Production and ultrastructure of lysozyme and ethylenediaminetetraacetate-lysozyme spheroplasts of Escherichia coli. J Bacteriol. 1967 Jan;93(1):427–437. doi: 10.1128/jb.93.1.427-437.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bridges B. A., Ashwood-Smith M. J., Munson R. J. Correlation of bacterial sensitivities to ionizing radiation and mild heating. J Gen Microbiol. 1969 Sep;58(1):115–124. doi: 10.1099/00221287-58-1-115. [DOI] [PubMed] [Google Scholar]
  4. COTA-ROBLES E. H. ELECTRON MICROSCOPY OF PLASMOLYSIS IN ESCHERICHIA COLI. J Bacteriol. 1963 Mar;85:499–503. doi: 10.1128/jb.85.3.499-503.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. De Petris S. Ultrastructure of the cell wall of Escherichia coli and chemical nature of its constituent layers. J Ultrastruct Res. 1967 Jul;19(1):45–83. doi: 10.1016/s0022-5320(67)80059-5. [DOI] [PubMed] [Google Scholar]
  6. Harries D., Russell A. D. A note on some changes in the physical properties of Escherichia coli after heat treatment. J Pharm Pharmacol. 1967 Nov;19(11):740–743. doi: 10.1111/j.2042-7158.1967.tb08026.x. [DOI] [PubMed] [Google Scholar]
  7. Heppel L. A. Selective release of enzymes from bacteria. Science. 1967 Jun 16;156(3781):1451–1455. doi: 10.1126/science.156.3781.1451. [DOI] [PubMed] [Google Scholar]
  8. Leive L., Shovlin V. K., Mergenhagen S. E. Physical, chemical, and immunological properties of lipopolysaccharide released from Escherichia coli by ethylenediaminetetraacetate. J Biol Chem. 1968 Dec 25;243(24):6384–6391. [PubMed] [Google Scholar]
  9. Russell A. D., Harries D. Damage to Escherichia coli on exposure to moist heat. Appl Microbiol. 1968 Sep;16(9):1394–1399. doi: 10.1128/am.16.9.1394-1399.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. STRANGE R. E., SHON M. EFFECTS OF THERMAL STRESS ON VIABILITY AND RIBONUCLEIC ACID OF AEROBACTER AEROGENES IN AQUEOUS SUSPENSION. J Gen Microbiol. 1964 Jan;34:99–114. doi: 10.1099/00221287-34-1-99. [DOI] [PubMed] [Google Scholar]
  11. Scheie P. O. Plasmolysis of Escherichia coli B-r with sucrose. J Bacteriol. 1969 May;98(2):335–340. doi: 10.1128/jb.98.2.335-340.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Scheie P. O., Rehberg R. Response of Escherichia coli B-r to high concentrations of sucrose in a nutrient medium. J Bacteriol. 1972 Jan;109(1):229–235. doi: 10.1128/jb.109.1.229-235.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Scheie P., Dalen H. Spatial anisotropy in Escherichia coli. J Bacteriol. 1968 Oct;96(4):1413–1414. doi: 10.1128/jb.96.4.1413-1414.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Woodcock E., Grigg G. W. Repair of thermally induced DNA breakage in Escherichia coli. Nat New Biol. 1972 May 17;237(72):76–79. doi: 10.1038/newbio237076a0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES