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. 1973 May;114(2):804–813. doi: 10.1128/jb.114.2.804-813.1973

Micrococcus lysodeikticus Bacterial Walls as a Substrate Specific for the Autolytic Glycosidase of Bacillus subtilis

David P Fan 1, Mary M Beckman 1
PMCID: PMC251842  PMID: 4196257

Abstract

The Bacillus subtilis 168 autolytic glycosidase degrades Micrococcus lysodeikticus cells or cell walls, whereas the B. subtilis autolytic amidase does not. The criteria used to establish this fact included: the determination of chemical bonds broken, heat-inactivated kinetics, pH dependence curves, and the physical separation of glycosidase from amidase. The physical separation involved LiCl elution from two different ion-exchange materials, walls from B. subtilis 168 strain βAO, and walls from mutant strain βA173 derived from strain βAO. No evidence was obtained for B. subtilis vegetative bacteria making any more autolysins than one autolytic amidase and one autolytic glycosidase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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