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. 1967 Sep;94(3):700–706. doi: 10.1128/jb.94.3.700-706.1967

Effect of Rec− Mutations on the Activity of Colicinogenic Factors

Donald R Helinski 1, Harvey R Herschman 1
PMCID: PMC251941  PMID: 5340681

Abstract

The effect of two Rec− mutations (AB2463 and JC1553) on the ability of a cell to accept, maintain, and express the colicinogenic factors ColE1, ColE2, and ColV was examined in Escherichia coli. These mutations had no observable effect on the colicinogenic properties of the ColV factor, but prevented the spontaneous and induced production of colicins E1 and E2 which are determined by the ColE1 and ColE2 factors, respectively. The two Rec− mutations had no apparent effect on the ability of the cells to acquire, maintain, or transfer the ColE1 and ColE2 factors. These mutations did not affect the expression of immunity by any of the three Col factors. ColE1 and ColE2 were also shown to be indirectly induced by mating F− cells carrying these Col factors with ultraviolet-irradiated, non-colicinogenic, Hfr and F+ cells. Indirect induction of colicin production occurred when either an irradiated F+ Rec+ or F+ Rec− strain was employed as the donor strain.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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