Abstract
The phospholipids of Hyphomicrobium vulgare NQ-521 have been separated by preparative thin-layer chromatography and analyzed by paper chromotography of the water-soluble products of acid and mild alkaline hydrolysis. The principal phospholipids are phosphatidyl ethanolamine (23%), phosphatidyl N,N′-dimethylethanolamine (36%), lecithin (29%), and phosphatidyl glycerol (10%). Three other strains of Hyphomicrobium were found to have similar phospholipid compositions. Growing cells incorporated the methyl group of methionine into lipid-bound N,N′-dimethylethanolamine and choline. Experiments with sonic extracts of H. vulgare NQ-521 and 14C (methyl) S-adenosylmethionine demonstrated the formation of phosphatidyl N-monomethylethanolamine in addition to the dimethylethanolamine and choline phosphatides.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- BAUMANN N. A., HAGEN P. O., GOLDFINE H. PHOSPHOLIPIDS OF CLOSTRIDIUM BUTYRICUM. STUDIES ON PLASMALOGEN COMPOSITION AND BIOSYNTHESIS. J Biol Chem. 1965 Apr;240:1559–1567. [PubMed] [Google Scholar]
- CONTI S. F., HIRSCH P. BIOLOGY OF BUDDING BACTERIA. 3. FINE STRUCTURE OF RHODOMICROBIUM AND HYPHOMICROBIUM SPP. J Bacteriol. 1965 Feb;89:503–512. doi: 10.1128/jb.89.2.503-512.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GIBSON K. D., WILSON J. D., UDENFRIEND S. The enzymatic conversion of phospholipid ethanolamine to phospholipid choline in rat liver. J Biol Chem. 1961 Mar;236:673–679. [PubMed] [Google Scholar]
- GOLDFINE H., BLOCH K. On the origin of unsaturated fatty acids in clostridia. J Biol Chem. 1961 Oct;236:2596–2601. [PubMed] [Google Scholar]
- GOLDFINE H., ELLIS M. E. N-METHYL GROUPS IN BACTERIAL LIPIDS. J Bacteriol. 1964 Jan;87:8–15. doi: 10.1128/jb.87.1.8-15.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GOLDFINE H. The characterization and biosynthesis of an N-methylethanolamine phospholipid from Clostridium butyricum. Biochim Biophys Acta. 1962 May 21;59:504–506. doi: 10.1016/0006-3002(62)90212-3. [DOI] [PubMed] [Google Scholar]
- GORCHEIN A. ORNITHINE IN RHODOPSEUDOMONAS SPHEROIDES. Biochim Biophys Acta. 1964 Jun 15;84:356–358. doi: 10.1016/0926-6542(64)90064-2. [DOI] [PubMed] [Google Scholar]
- Goldfine H. Use of a filter-paper disk assay in the measurement of lipid biosynthesis. J Lipid Res. 1966 Jan;7(1):146–149. [PubMed] [Google Scholar]
- HANES C. S., ISHERWOOD F. A. Separation of the phosphoric esters on the filter paper chromatogram. Nature. 1949 Dec 31;164(4183):1107-12, illust. doi: 10.1038/1641107a0. [DOI] [PubMed] [Google Scholar]
- HIRSCH P., CONTI S. F. BIOLOGY OF BUDDING BACTERIA. I. ENRICHMENT, ISOLATION AND MORPHOLOGY OF HYPHOMICROBIUM SPP. Arch Mikrobiol. 1964 Jun 26;48:339–357. doi: 10.1007/BF00405978. [DOI] [PubMed] [Google Scholar]
- HIRSCH P., CONTI S. F. BIOLOGY OF BUDDING BACTERIA. II. GROWTH AND NUTRITION OF HYPHOMICROBIUM SPP. Arch Mikrobiol. 1964 Jun 26;48:358–367. doi: 10.1007/BF00405979. [DOI] [PubMed] [Google Scholar]
- Hagen P. O., Goldfine H., Williams P. J. Phospholipids of bacteria with extensive intracytoplasmic membranes. Science. 1966 Mar 25;151(3717):1543–1544. doi: 10.1126/science.151.3717.1543. [DOI] [PubMed] [Google Scholar]
- Ikawa M. Bacterial phosphatides and natural relationships. Bacteriol Rev. 1967 Mar;31(1):54–64. doi: 10.1128/br.31.1.54-64.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JONES G. E., BENSON A. A. PHOSPHATIDYL GLYCEROL IN THIOBACILLUS THIOOXIDANS. J Bacteriol. 1965 Jan;89:260–261. doi: 10.1128/jb.89.1.260-261.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KANESHIRO T., LAW J. H. PHOSPHATIDYLCHOLINE SYNTHESIS IN AGROBACTERIUM TUMEFACIENS. I. PURIFICATION AND PROPERTIES OF A PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASE. J Biol Chem. 1964 Jun;239:1705–1713. [PubMed] [Google Scholar]
- Kates M. Bacterial lipids. Adv Lipid Res. 1964;2:17–90. [PubMed] [Google Scholar]
- LASCELLES J., SZILAGYI J. F. PHOSPHOLIPID SYNTHESIS BY RHODOPSEUDOMONAS SPHEROIDES IN RELATION TO THE FORMATION OF PHOTOSYNTHETIC PIGMENTS. J Gen Microbiol. 1965 Jan;38:55–64. doi: 10.1099/00221287-38-1-55. [DOI] [PubMed] [Google Scholar]
- Lust G., Daniel L. J. The biosynthesis of the methyl groups of choline in Ochromonas malhamensis. Arch Biochem Biophys. 1966 Mar;113(3):603–608. doi: 10.1016/0003-9861(66)90237-2. [DOI] [PubMed] [Google Scholar]
- MURRAY R. G., WATSON S. W. STRUCTURE OF NITROSOCYSTIS OCEANUS AND COMPARISON WITH NITROSOMONAS AND NITROBACTER. J Bacteriol. 1965 Jun;89:1594–1609. doi: 10.1128/jb.89.6.1594-1609.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Park C. E., Berger L. R. Complex lipids of Rhodomicrobium vannielii. J Bacteriol. 1967 Jan;93(1):221–229. doi: 10.1128/jb.93.1.221-229.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Scarborough G. A., Nyc J. F. Methylation of ethanolamine phosphatides by microsomes from normal and mutant strains of Neurospora crassa. J Biol Chem. 1967 Jan 25;242(2):238–242. [PubMed] [Google Scholar]
- TARLOV A. R., KENNEDY E. P. THE BETA-GALACTOSIDE PERMEASE SYSTEM AND THE METABOLISM OF PHOSPHOLIPIDS IN ESCHERICHIA COLI. J Biol Chem. 1965 Jan;240:49–53. [PubMed] [Google Scholar]
- Wood B. J., Nichols B. W., James A. T. The lipids and fatty acid metabolism of photosynthetic bacteria. Biochim Biophys Acta. 1965 Oct 4;106(2):261–273. doi: 10.1016/0005-2760(65)90034-2. [DOI] [PubMed] [Google Scholar]
- van Iterson W. Symposium on the fine structure and replication of bacteria and their parts. II. Bacterial cytoplasm. Bacteriol Rev. 1965 Sep;29(3):299–325. doi: 10.1128/br.29.3.299-325.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]