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. 1968 Feb;95(2):559–564. doi: 10.1128/jb.95.2.559-564.1968

Chemical and Serological Relationships Between the Heteropolysaccharides of Mycobacterium tuberculosis and Mycobacterium kansasii1

Susan E Birnbaum a, Lewis F Affronti a
PMCID: PMC252052  PMID: 4966549

Abstract

The identity of a heteropolysaccharide from cell walls of Mycobacterium tuberculosis H37Ra with Seibert's tuberculopolysaccharide I was demonstrated by thin-layer chromatography, chemical analysis, and antigenic tests. The polysaccharide of M. kansasii was shown to be identical with that of M. tuberculosis. Defatted cells were disintegrated by ultrasonic treatment in the presence of glass beads; cell walls were obtained by differential ultracentrifugation. Ethyl alcohol-precipitated carbohydrate extracts were analyzed for protein and nucleic acid; these impurities were removed. Tuberculopolysaccharide I from the mycobacterial culture filtrate is probably derived from a lipopolysaccharide of the cell wall, which is partially removed by chloroform in the intact state. Alkaline extraction releases additional polysaccharide, in varying degrees of association with cell wall murein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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