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. 1968 Feb;95(2):612–617. doi: 10.1128/jb.95.2.612-617.1968

Identification of the Toxic Component of Brucella abortus Endotoxin and Its Labeling with Radioactive Chromate

Daniel Leong a,1, Ramon Diaz a, J B Wilson a
PMCID: PMC252059  PMID: 4966552

Abstract

The evidence obtained from immuno-gel diffusion, centrifugation, and toxicity studies employing the serum iron assay proved that most of the toxicity in an ether-water brucella endotoxin preparation lies in the slow-diffusing component identified as the biologically active endotoxin. Subsequent destruction of the slow-diffusing component by acid hydrolysis resulted in a corresponding loss of toxicity. Chromate-51 was found to attach almost entirely on the slow-diffusing biologically active component and, hence, is a valid label for endotoxin derived from smooth Brucella abortus by the ether-water method.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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