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. 1968 Mar;95(3):824–832. doi: 10.1128/jb.95.3.824-832.1968

Regulation of Pyrimidine Biosynthesis in Saccharomyces cerevisiae1

Francois Lacroute a,2
PMCID: PMC252099  PMID: 5651325

Abstract

Biochemical steps of the pyrimidine pathway have been found to be the same in yeast as in bacteria, and all except one step have been characterized. The activities of the first two enzymes, carbamoyl phosphate synthetase and aspartic transcarbamylase, are simultaneously controlled by feedback inhibition and repression. Moreover, these enzymes are coded by the same genetic region (ura-2) and seem to form a single enzymatic complex. The enzymes that follow later in the pathway are induced in a sequential way by the intermediary products and are insensitive to pyrimidine repression. The corresponding genes (ura-4, ura-1, ura-3) are not linked to each other or to ura-2, the gene for carbamoyl phosphate synthetase and aspartic transcarbamylase. Mutants that have simultaneously lost feedback inhibition by uridine triphosphate for carbamoyl phosphate synthetase and for aspartic transcarbamylase have been found and mapped in the gene ura-2.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BECKWITH J. R., PARDEE A. B., AUSTRIAN R., JACOB F. Coordination of the synthesis of the enzymes in the pyrimidine pathway of E. coli. J Mol Biol. 1962 Dec;5:618–634. doi: 10.1016/s0022-2836(62)80090-4. [DOI] [PubMed] [Google Scholar]
  2. COHEN G. N., PATTE J. C., TRUFFA-BACHI P. PARALLEL MODIFICATIONS CAUSED BY MUTATIONS IN TWO ENZYMES CONCERNED WITH THE BIOSYNTHESIS OF THREONINE IN ESCHERICHIA COLI. Biochem Biophys Res Commun. 1965 May 3;19:546–550. doi: 10.1016/0006-291x(65)90160-9. [DOI] [PubMed] [Google Scholar]
  3. DAVIS R. H., WOODWARD V. W. The relationship between gene suppression and aspartate transcarbamylase activity in pyr-3 mutants of Neurospora. Genetics. 1962 Aug;47:1075–1083. doi: 10.1093/genetics/47.8.1075. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. GERHART J. C., PARDEE A. B. The enzymology of control by feedback inhibition. J Biol Chem. 1962 Mar;237:891–896. [PubMed] [Google Scholar]
  5. Gross S. R. The regulation of synthesis of leucine biosynthetic enzymes in Neurospora. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1538–1546. doi: 10.1073/pnas.54.6.1538. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. KORITZ S. B., COHEN P. P. Colorimetric determination of carbamylamino acids and related compounds. J Biol Chem. 1954 Jul;209(1):145–150. [PubMed] [Google Scholar]
  7. KROOTH R. S. PROPERTIES ODF DIPLOID CELL STRAINS DEVELOPED FROM PATIENTS WITH AN INHERITED ABNORMALITY OF URIDINE BIOSYNTHESIS. Cold Spring Harb Symp Quant Biol. 1964;29:189–212. doi: 10.1101/sqb.1964.029.01.024. [DOI] [PubMed] [Google Scholar]
  8. LACROUTE F., SLONIMSKI P. P. ETUDE PHYSIOLOGIQUE DES MUTANTS R'ESISTANT AU 5-FLUOROURACILE CHEZ LA LEVURE. C R Hebd Seances Acad Sci. 1964 Feb 17;258:2172–2174. [PubMed] [Google Scholar]
  9. LACROUTE F. UN CAS DE DOUBLE R'ETROCONTR OLE: LA CHA INE DE BIOSYNTH'ESE DE L'URACILE CHEZ LA LEVURE. C R Hebd Seances Acad Sci. 1964 Aug 10;259:1357–1359. [PubMed] [Google Scholar]
  10. Lacroute F., Piérard A., Grenson M., Wiame J. M. The biosynthesis of carbamoyl phosphate in Saccharomyces cerevisiae. J Gen Microbiol. 1965 Jul;40(1):127–142. doi: 10.1099/00221287-40-1-127. [DOI] [PubMed] [Google Scholar]
  11. Mortimer R. K., Hawthorne D. C. Genetic mapping in Saccharomyces. Genetics. 1966 Jan;53(1):165–173. doi: 10.1093/genetics/53.1.165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Pinsky L., Krooth R. S. Studies on the control of pyrimidine biosynthesis in human diploid cell strains. II. Effects of 5-azaorotic acid, barbituric acid, and pyrimidine precursors on cellular phenotype. Proc Natl Acad Sci U S A. 1967 May;57(5):1267–1274. doi: 10.1073/pnas.57.5.1267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. SOMLO M. [L-lactic dehydrogenase in aerobic yeast. Comparison of the properties of the bound enzyme and the soluble enzyme]. Biochim Biophys Acta. 1962 Dec 4;65:333–346. doi: 10.1016/0006-3002(62)91052-1. [DOI] [PubMed] [Google Scholar]
  14. Stanier R. Y. Simultaneous Adaptation: A New Technique for the Study of Metabolic Pathways. J Bacteriol. 1947 Sep;54(3):339–348. doi: 10.1128/jb.54.3.339-348.1947. [DOI] [PMC free article] [PubMed] [Google Scholar]

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