Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1968 Mar;95(3):932–936. doi: 10.1128/jb.95.3.932-936.1968

Regulation of Pentitol Metabolism by Aerobacter aerogenes II. Induction of the Ribitol Pathway

Theresa M Bisson 1, Eugene J Oliver 1, Robert P Mortlock 1
PMCID: PMC252112  PMID: 5643066

Abstract

The incubation of Aerobacter aerogenes PRL-R3 with ribitol resulted in the induction of ribitol dehydrogenase and d-ribulokinase, coordinately controlled enzymes of the pathway of ribitol catabolism. A dehydrogenase-negative mutant was unable to induce d-ribulokinase activity following incubation with ribitol. Similar experiments using a kinase-negative mutant resulted in normal induction of ribitol dehydrogenase, as compared to the wild-type PRL-R3 strain. Constitutive or induced cells for l-fucose isomerase were capable of catalyzing the isomerization of d-arabinose to d-ribulose. In contrast to the experiments using ribitol as the substrate, the isomerization of d-arabinose resulted in the induction of d-ribulokinase with dehydrogenase-negative cells. These data indicated that d-ribulose, rather than ribitol, acts as the inducer of the enzymes for ribitol degradation.

Full text

PDF
933

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDERSON R. L., WOOD W. A. Pathway of L-xylose and L-lyxose degradation in Aerobacter aerogenes. J Biol Chem. 1962 Feb;237:296–303. [PubMed] [Google Scholar]
  2. Camyre K. P., Mortlock R. P. Growth of Aerobacter aerogenes on D-arabinose and L-xylose. J Bacteriol. 1965 Oct;90(4):1157–1158. doi: 10.1128/jb.90.4.1157-1158.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DISCHE Z., BORENFREUND E. A new spectrophotometric method for the detection and determination of keto sugars and trioses. J Biol Chem. 1951 Oct;192(2):583–587. [PubMed] [Google Scholar]
  4. KOCH J. P., HAYASHI S., LIN E. C. THE CONTROL OF DISSIMILATION OF GLYCEROL AND L-ALPHA-GLYCEROPHOSPHATE IN ESCHERICHIA COLI. J Biol Chem. 1964 Sep;239:3106–3108. [PubMed] [Google Scholar]
  5. MORTLOCK R. P., FOSSITT D. D., PETERING D. H., WOOD W. A. METABOLISM OF PENTOSES AND PENTITOLS BY AEROBACTER AEROGENES. 3. PHYSICAL AND IMMUNOLOGICAL PROPERTIES OF PENITOL DEHYDROGENASES AND PENTULOKINASES. J Bacteriol. 1965 Jan;89:129–135. doi: 10.1128/jb.89.1.129-135.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. MORTLOCK R. P., WOOD W. A. METABOLISM OF PENTOSES AND PENTITOLS BY AEROBACTER AEROGENES. I. DEMONSTRATION OF PENTOSE ISOMERASE, PENTULOKINASE, AND PENTITOL DEHYDROGENASE ENZYME FAMILIES. J Bacteriol. 1964 Oct;88:838–844. doi: 10.1128/jb.88.4.838-844.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. MORTLOCK R. P., WOOD W. A. METABOLISM OF PENTOSES AND PENTITOLS BY AEROBACTER AEROGENES. II. MECHANISM OF ACQUISITION OF KINASE, ISOMERASE, AND DEHYDROGENASE ACTIVITY. J Bacteriol. 1964 Oct;88:845–849. doi: 10.1128/jb.88.4.845-849.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Magasanik B., Lund P., Neidhardt F. C., Schwartz D. T. Induction and repression of the histidine-degrading enzymes in Aerobacter aerogenes. J Biol Chem. 1965 Nov;240(11):4320–4324. [PubMed] [Google Scholar]
  9. PALLERONI N. J., STANIER R. Y. REGULATORY MECHANISMS GOVERNING SYNTHESIS OF THE ENZYMES FOR TRYPTOPHAN OXIDATION BY PSEUDOMONAS FLUORESCENS. J Gen Microbiol. 1964 May;35:319–334. doi: 10.1099/00221287-35-2-319. [DOI] [PubMed] [Google Scholar]
  10. Schlesinger S., Scotto P., Magasanik B. Exogenous and endogenous induction of the histidine-degrading enzymes in Aerobacter aerogenes. J Biol Chem. 1965 Nov;240(11):4331–4337. [PubMed] [Google Scholar]
  11. WOOD W. A., McDONOUGH M. J., JACOBS L. B. Rihitol and D-arabitol utilization by Aerobacter aerogenes. J Biol Chem. 1961 Aug;236:2190–2195. [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES