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. 1968 Mar;95(3):1024–1029. doi: 10.1128/jb.95.3.1024-1029.1968

Cystathionine Metabolism in Methionine Auxotrophic and Wild-Type Strains of Saccharomyces cerevisiae

W A Sorsoli 1, M Buettner 1, L W Parks 1
PMCID: PMC252127  PMID: 5643045

Abstract

The role of cystathionine in methionine biosynthesis in wild-type and auxotrophic strains of Saccharomyces cerevisiae was studied. Homocysteine and cysteinerequiring mutants were selected for detailed study. Exogenously supplied cystathionine, although actively transported by all strains tested, could not satisfy the organic sulfur requirements of the mutants. Cell-free extracts of the wild-type, homocysteine, and cysteine auxotrophs were shown to cleave cystathionine. Pyruvic acid and homocysteine were identified as teh products of this cleavage. A mutant containing an enzyme which could cleave cystathionine to homocysteine in cell-free experiments was unable to use cystathionine as a methionine precursor in the intact organisms. The significance of this finding is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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