Abstract
The effects of starving Escherichia coli for glucose and required amino acids were determined. The disappearance of the majority of the ribosomal population, particularly of the ribonucleic acid (RNA) moiety, was noted. The events during recovery are detailed, with emphasis placed on the timing, requirements, and control of deoxyribonucleic acid, RNA, and protein synthesis and on ribosome reassembly. Finally, the applicability of the system to the study of temperature-sensitive mutants is documented and discussed.
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Selected References
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