Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1968 May;95(5):1721–1726. doi: 10.1128/jb.95.5.1721-1726.1968

Regulation of Nitrogen Fixation in Azotobacter vinelandii OP and in an Apparently Partially Constitutive Mutant

George J Sorger 1
PMCID: PMC252202  PMID: 5650079

Abstract

Methylamine and 2-methylalanine appeared to act as co-repressors of nitrogenase in Azotobacter vinelandii OP. They inhibited the growth of this organism on molecular nitrogen but not on nitrate, ammonia, or Casamino Acids; they prevented the formation of nitrogenase by cells transferred from repression to induction conditions; and they did not inhibit the activity of nitrogenase in vitro. A mutant of strain OP, selected on the basis of its relative resistance to methylalanine, appeared partially constitutive because nitrogenase in this strain was less sensitive to repressors than was the enzyme in the wild-type strain.

Full text

PDF
1722

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BULEN W. A., BURNS R. C., LECOMTE J. R. NITROGEN FIXATION: HYDROSULFITE AS ELECTRON DONOR WITH CELL-FREE PREPARATIONS OF AZOTOBACTER VINELANDII AND RHODOSPIRILLUM RUBRUM. Proc Natl Acad Sci U S A. 1965 Mar;53:532–539. doi: 10.1073/pnas.53.3.532. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DE WITT C. W., ROWE J. A. N,O-Diacetylneuraminic acid and N-acetylneuraminic acid in Escherichia coli. Nature. 1959 Aug 1;184(Suppl 6):381–382. doi: 10.1038/184381b0. [DOI] [PubMed] [Google Scholar]
  3. GEST H., ORMEROD J. G., ORMEROD K. S. Photometabolism of Rhodospirillum rubrum: light-dependent dissimilation of organic compounds to carbon dioxide and molecular hydrogen by an anaerobic citric acid cycle. Arch Biochem Biophys. 1962 Apr;97:21–33. doi: 10.1016/0003-9861(62)90039-5. [DOI] [PubMed] [Google Scholar]
  4. GREEN M., WILSON P. W. Hydrogenase and nitrogenase in Azotobacter. J Bacteriol. 1953 May;65(5):511–517. doi: 10.1128/jb.65.5.511-517.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gest H., Kamen M. D. STUDIES ON THE METABOLISM OF PHOTOSYNTHETIC BACTERIA IV. : Photochemical Production of Molecular Hydrogen by Growing Cultures of Photosynthetic Bacteria. J Bacteriol. 1949 Aug;58(2):239–245. [PMC free article] [PubMed] [Google Scholar]
  6. HAMILTON I. R., BURRIS R. H., WILSON P. W. HYDROGENASE AND NITROGENASE IN A NITROGEN-FIXING BACTERIUM. Proc Natl Acad Sci U S A. 1964 Sep;52:637–641. doi: 10.1073/pnas.52.3.637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hardy R. W., Knight E., Jr ATP-dependent reduction of azide and HCN by N2-fixing enzymes of Azotobacter vinelandii and Clostridium pasteurianum. Biochim Biophys Acta. 1967 May 16;139(1):69–90. doi: 10.1016/0005-2744(67)90114-3. [DOI] [PubMed] [Google Scholar]
  8. Hardy R. W., Knight E., Jr, D'Eustachio A. J. An energy-dependent hydrogen-evolution from dithionite in nitrogen-fixing extracts of Clostridium pasteurianum. Biochem Biophys Res Commun. 1965 Sep 8;20(5):539–544. doi: 10.1016/0006-291x(65)90431-6. [DOI] [PubMed] [Google Scholar]
  9. Mortenson L. E., Morris J. A., Jeng D. Y. Purification, metal composition and properties of molybdoferredoxin and azoferredoxin, two of the components of the nitrogen-fixing system of Clostridium pasteurianum. Biochim Biophys Acta. 1967 Aug 29;141(3):516–522. doi: 10.1016/0304-4165(67)90180-8. [DOI] [PubMed] [Google Scholar]
  10. NICHOLAS D. J., WILSON P. W., HEINEN W., PALMER G., BEINERT H. Use of electron paramagnetic resonance spectroscopy in investigations of functional metal components in micro-organisms. Nature. 1962 Nov 3;196:433–436. doi: 10.1038/196433a0. [DOI] [PubMed] [Google Scholar]
  11. ORMEROD J. G., ORMEROD K. S., GEST H. Light-dependent utilization of organic compounds and photoproduction of molecular hydrogen by photosynthetic bacteria; relationships with nitrogen metabolism. Arch Biochem Biophys. 1961 Sep;94:449–463. doi: 10.1016/0003-9861(61)90073-x. [DOI] [PubMed] [Google Scholar]
  12. PENGRA R. M., WILSON P. W. Physiology of nitrogen fixation by Aerobacter aerogenes. J Bacteriol. 1958 Jan;75(1):21–25. doi: 10.1128/jb.75.1.21-25.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Patil R. B., Pengra R. M., Yoch D. C. Effects of nitrogen supplements on nitrogen fixation by Aerobacter aerogenes. Biochim Biophys Acta. 1967 Feb 7;136(1):1–5. doi: 10.1016/0304-4165(67)90314-5. [DOI] [PubMed] [Google Scholar]
  14. WILSON P. W., BURRIS R. H. Biological nitrogen fixation; a reappraisal. Annu Rev Microbiol. 1953;7:415–432. doi: 10.1146/annurev.mi.07.100153.002215. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES