Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1968 May;95(5):1844–1850. doi: 10.1128/jb.95.5.1844-1850.1968

Ribosome Development and the Methylation of Ribosomal Ribonucleic Acid

Paul S Sypherd 1
PMCID: PMC252219  PMID: 4870284

Abstract

Immature ribonucleoprotein particles accumulate in amino acid-starved cells of a relaxed mutant of Escherichia coli. The ribonucleic acid (RNA) of these particles is nonmethylated in cells starved for methionine. However, in bacteria starved for arginine, lysine, or histidine, the RNA of these particles is one-half methylated. The relationship of submethylation to a structural alteration in the same RNA was studied. The results of kinetic studies showed that submethylation and the structural transition are not causally related, since they are described by different rate constants. Moreover, it was possible to accumulate fully methylated immature-particle RNA that possessed the sedimentation and chromatographic properties of nonmethylated RNA. It was concluded that, during the normal course of ribosome development, methylation of ribosomal RNA is completed prior to the final maturation steps.

Full text

PDF
1847

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRITTEN R. J., McCARTHY B. J. The synthesis of ribosomes in E. coli. II. Analysis of the kinetics of tracer incorporation in growing cells. Biophys J. 1962 Jan;2:49–55. doi: 10.1016/s0006-3495(62)86840-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DUBIN D. T., ELKORT A. T. SOME ABNORMAL PROPERTIES OF CHLORAMPHENICOL RNA. J Mol Biol. 1964 Dec;10:508–518. doi: 10.1016/s0022-2836(64)80069-3. [DOI] [PubMed] [Google Scholar]
  3. DUBIN D. T. SOME EFFECT OF STREPTOMYCIN ON RNA METABOLISM IN ESCHERICHIA COLI. J Mol Biol. 1964 May;8:749–767. doi: 10.1016/s0022-2836(64)80122-4. [DOI] [PubMed] [Google Scholar]
  4. FLEISSNER E., BOREK E. STUDIES ON THE ENZYMATIC METHYLATION OF SOLUBLE RNA. I. METHYLATION OF THE S-RNA POLYMER. Biochemistry. 1963 Sep-Oct;2:1093–1100. doi: 10.1021/bi00905a032. [DOI] [PubMed] [Google Scholar]
  5. GORDON J., BOMAN H. G. STUDIES ON MICROBIAL RNA.II. TRANSFER OF METHYL GROUPS FROM METHIONINE TO THE RNA OF A RIBONUCLEOPROTEIN PARTICLE. J Mol Biol. 1964 Sep;9:638–653. doi: 10.1016/s0022-2836(64)80172-8. [DOI] [PubMed] [Google Scholar]
  6. Gesteland R. F. Isolation and characterization of ribonuclease I mutants of Escherichia coli. J Mol Biol. 1966 Mar;16(1):67–84. doi: 10.1016/s0022-2836(66)80263-2. [DOI] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Manor H., Haselkorn R. Properties of ribonucleic acid components in ribonucleoprotein particle preparations obtained from an Escherichia coli RC-relaxed strain. J Mol Biol. 1967 Mar 14;24(2):269–288. doi: 10.1016/0022-2836(67)90332-4. [DOI] [PubMed] [Google Scholar]
  9. SYPHERD P. S. ACCUMULATION OF RIBONUCLEOPROTEIN PARTICLES IN A RELAXED MUTANT OF ESCHERICHIA COLI. J Bacteriol. 1965 Aug;90:403–410. doi: 10.1128/jb.90.2.403-410.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. SYPHERD P. S. FORMATION OF RIBOSOMES FROM PRECURSOR RIBONUCLEOPROTEIN PARTICLES. J Bacteriol. 1965 Aug;90:411–417. doi: 10.1128/jb.90.2.411-417.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Srinivasan P. R., Borek E. Enzymatic alteration of macromolecular structure. Prog Nucleic Acid Res Mol Biol. 1966;5:157–189. doi: 10.1016/s0079-6603(08)60234-2. [DOI] [PubMed] [Google Scholar]
  12. Sypherd P. S., Fansler B. S. Structural transitions in ribonucleic acid during ribosome development. J Bacteriol. 1967 Mar;93(3):920–929. doi: 10.1128/jb.93.3.920-929.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sypherd P. S. Site of synthesis of ribosomal proteins. J Mol Biol. 1966 Jun;17(2):358–369. doi: 10.1016/s0022-2836(66)80147-x. [DOI] [PubMed] [Google Scholar]
  14. Sypherd P. S., Strauss N. CHLORAMPHENICOL-PROMOTED REPRESSION OF beta-GALACTOSIDASE SYNTHESIS IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1963 Mar;49(3):400–407. doi: 10.1073/pnas.49.3.400. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. YANKOFSKY S. A., SPIEGELMAN S. Distinct cistrons for the two ribosomal RNA components. Proc Natl Acad Sci U S A. 1963 Apr;49:538–544. doi: 10.1073/pnas.49.4.538. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES