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. 1968 May;95(5):1857–1861. doi: 10.1128/jb.95.5.1857-1861.1968

Induction and Propagation of a Bacillus subtilis L Form in Natural and Synthetic Media

H R Burmeister 1, C W Hesseltine 1
PMCID: PMC252221  PMID: 4967777

Abstract

The L form of Bacillus subtilis NRRL B-3275 was induced in a 7% NaCl broth medium and subsequently propagated in natural and synthetic media. The L form grew readily in tryptone broth supplemented with glucose, NaCl, and phosphate buffer, and in a synthetic medium containing only glucose and biotin, in addition to the required salts. Successive transfers from the bacillus inoculum and subsequent large bodies in the tryptone broth with 7% NaCl resulted in gradual selection or transition from the bacillary form to a stable L form without the addition of an antibiotic. The number of viable granules attained in the broth culture exceeded 9 × 107 per ml, and numerous large bodies were always present in rapidly growing cultures.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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