Abstract
When Shigella dysenteriae strain 60 is used as a donor and Escherichia coli K-12 strains that are ultraviolet (UV)-sensitive, mucoid, and proline-requiring (Pro−) are employed as recipients, selection for Pro+ yields 2 to 6% nonmucoid clones. All of the nonmucoid clones examined are UV-resistant. Most of the nonmucoid UV-resistant transductants are partial diploids for the genes being studied. When these Shigella-Escherichia hybrids are used as donors with the same E. coli recipients, the cotransduction of Pro+ and nonmucoidness is greatly increased (59 to 94% cotransduction). All of these nonmucoid transductants examined were also UV-resistant. The results indicate that Shigella contains an allele (designated ShproC+) homologous to proC of E. coli and a second linked allele (designated ShcapR+) homologous to the capR allele of E. coli. The ShcapR+ allele changes the phenotype of certain E. coli strains from mucoid UV-sensitive (capR6) or very sensitive (capR9) to nonmucoid and UV-resistant. Unanticipated capR allele interactions in the partial diploid hybrids are described.
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