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. 1968 Aug;96(2):462–471. doi: 10.1128/jb.96.2.462-471.1968

Analysis of Melibiose Mutants Deficient in α-Galactosidase and Thiomethylgalactoside Permease II in Escherichia coli K-12

Rüdiger Schmitt a,1
PMCID: PMC252319  PMID: 4877127

Abstract

Three types of mutants (mel−) unable to metabolize the α-d-galactoside, melibiose, were derived from Escherichia coli K-12. One type lacked α-galactosidase; another lacked a specific transport system, termed thiomethylgalactoside (TMG) permease II; and the third lacked both of these functions. The mutational sites were genetically mapped by recombination frequency with different markers and by determination of chromosomal transfer in interrupted-mating experiments. All three mel− mutant types mapped in a cluster near to the metA marker on the E. coli chromosome and were cotransducible. Induction studies revealed that the three α-d-galactosides, melibiose, melibiitol, and galactinol, induced α-galactosidase and TMG permease II coordinately; d-galactose also induced them but only in a galactokinaseless mutant. These data suggest that α-galactosidase and TMG permease II may be components of a common operon.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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