Skip to main content
Journal of Virology logoLink to Journal of Virology
. 1985 Dec;56(3):1041–1044. doi: 10.1128/jvi.56.3.1041-1044.1985

Novel 2',5'-oligoadenylates synthesized in interferon-treated, vaccinia virus-infected cells.

A P Rice, S M Kerr, W K Roberts, R E Brown, I M Kerr
PMCID: PMC252683  PMID: 2415713

Abstract

2-5A[ppp(A2'p)nA] and related materials accumulate to greater than micromolar concentrations in vaccinia virus-infected HeLa cells and in interferon-treated, vaccinia-infected HeLa, CV1, and L929 cells even when virus replication is not inhibited. A variably complex mixture of authentic 2-5A (n = 2 to 4), nonphosphorylated cores [(A2'p)nA; n = 2 to 5], and additional compounds of unknown structure was observed.

Full text

PDF
1043

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benavente J., Paez E., Esteban M. Indiscriminate degradation of RNAs in interferon-treated, vaccinia virus-infected mouse L cells. J Virol. 1984 Sep;51(3):866–871. doi: 10.1128/jvi.51.3.866-871.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brown R. E., Cayley P. J., Kerr I. M. Analysis of (2'-5')-oligo(A) and related oligonucleotides by high-performance liquid chromatography. Methods Enzymol. 1981;79(Pt B):208–216. doi: 10.1016/s0076-6879(81)79031-1. [DOI] [PubMed] [Google Scholar]
  3. Cayley P. J., Davies J. A., McCullagh K. G., Kerr I. M. Activation of the ppp(A2'p)nA system in interferon-treated, herpes simplex virus-infected cells and evidence for novel inhibitors of the ppp(A2'p)nA-dependent RNase. Eur J Biochem. 1984 Aug 15;143(1):165–174. doi: 10.1111/j.1432-1033.1984.tb08355.x. [DOI] [PubMed] [Google Scholar]
  4. Cayley P. J., Kerr I. M. Synthesis, characterisation and biological significance of (2'-5')oligoadenylate derivatives of NAD+, ADP-ribose and adenosine(5')tetraphospho(5')adenosine. Eur J Biochem. 1982 Mar 1;122(3):601–608. [PubMed] [Google Scholar]
  5. Esteban M., Benavente J., Paez E. Effect of interferon on integrity of vaccinia virus and ribosomal RNA in infected cells. Virology. 1984 Apr 15;134(1):40–51. doi: 10.1016/0042-6822(84)90270-8. [DOI] [PubMed] [Google Scholar]
  6. Ferbus D., Justesen J., Besançon F., Thang M. N. The 2'5' oligoadenylate synthetase has a multifunctional 2'5' nucleotidyl-transferase activity. Biochem Biophys Res Commun. 1981 May 29;100(2):847–856. doi: 10.1016/s0006-291x(81)80251-3. [DOI] [PubMed] [Google Scholar]
  7. Goswami B. B., Sharma O. K. Degradation of rRNA in interferon-treated vaccinia virus-infected cells. J Biol Chem. 1984 Feb 10;259(3):1371–1374. [PubMed] [Google Scholar]
  8. Haugh M. C., Cayley P. J., Serafinowska H. T., Norman D. G., Reese C. B., Kerr I. M. Analogues and analogue inhibitors of ppp(A2'p)nA. Their stability and biological activity. Eur J Biochem. 1983 Apr 15;132(1):77–84. doi: 10.1111/j.1432-1033.1983.tb07327.x. [DOI] [PubMed] [Google Scholar]
  9. Hersh C. L., Brown R. E., Roberts W. K., Swyryd E. A., Kerr I. M., Stark G. R. Simian virus 40-infected, interferon-treated cells contain 2',5'-oligoadenylates which do not activate cleavage of RNA. J Biol Chem. 1984 Feb 10;259(3):1731–1737. [PubMed] [Google Scholar]
  10. Hersh C. L., Reid T. R., Friedman R., Stark G. R. Preparation, characterization, and use of an antiserum specific for 2',5'-oligoadenylates. J Biol Chem. 1984 Feb 10;259(3):1727–1730. [PubMed] [Google Scholar]
  11. Knight M., Cayley P. J., Silverman R. H., Wreschner D. H., Gilbert C. S., Brown R. E., Kerr I. M. Radioimmune, radiobinding and HPLC analysis of 2-5A and related oligonucleotides from intact cells. Nature. 1980 Nov 13;288(5787):189–192. doi: 10.1038/288189a0. [DOI] [PubMed] [Google Scholar]
  12. Lengyel P. Biochemistry of interferons and their actions. Annu Rev Biochem. 1982;51:251–282. doi: 10.1146/annurev.bi.51.070182.001343. [DOI] [PubMed] [Google Scholar]
  13. Minks M. A., Benvin S., Baglioni C. Mechanism of pppA(2'p5'A)n2'p5'AOH synthesis in extracts of interferon-treated HeLa cells. J Biol Chem. 1980 Jun 10;255(11):5031–5035. [PubMed] [Google Scholar]
  14. Paez E., Esteban M. Nature and mode of action of vaccinia virus products that block activation of the interferon-mediated ppp(A2'p)nA-synthetase. Virology. 1984 Apr 15;134(1):29–39. doi: 10.1016/0042-6822(84)90269-1. [DOI] [PubMed] [Google Scholar]
  15. Paez E., Esteban M. Resistance of vaccinia virus to interferon is related to an interference phenomenon between the virus and the interferon system. Virology. 1984 Apr 15;134(1):12–28. doi: 10.1016/0042-6822(84)90268-x. [DOI] [PubMed] [Google Scholar]
  16. Rice A. P., Roberts W. K., Kerr I. M. 2-5A accumulates to high levels in interferon-treated, vaccinia virus-infected cells in the absence of any inhibition of virus replication. J Virol. 1984 Apr;50(1):220–228. doi: 10.1128/jvi.50.1.220-228.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES