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. 1986 Jan;57(1):183–190. doi: 10.1128/jvi.57.1.183-190.1986

In vitro transcription of human pararotavirus.

M Jashés, A M Sandino, G Faúndez, L F Avendaño, E Spencer
PMCID: PMC252713  PMID: 3001343

Abstract

Purified human pararotavirus obtained from stool samples from a 6-month-old infant was characterized. Electron microscopy of the viral particles subjected to different treatments suggested that the protein shells differed from those described for rotavirus. Treatment with both EDTA or ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid in the presence or absence of Mg2+ seemed to convert the virions into core particles by removal of both the outer and inner shells, and no particles equivalent to single-shelled rotavirus were observed. Different procedures were used to activate the human pararotavirus-associated RNA-dependent RNA polymerase. The enzyme was not activated by chelating agents or by thermal shock as in rotavirus. Activation by thermal shock occurred only in the presence of the four ribonucleoside triphosphates and Mg2+. However, the polymerase of pararotavirus was found to be similar to those described for rotaviruses. When in vitro transcripts were analyzed, 11 RNA species having a migration pattern similar to that of the original genomic RNA were detected.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bican P., Cohen J., Charpilienne A., Scherrer R. Purification and characterization of bovine rotavirus cores. J Virol. 1982 Sep;43(3):1113–1117. doi: 10.1128/jvi.43.3.1113-1117.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bohl E. H., Saif L. J., Theil K. W., Agnes A. G., Cross R. F. Porcine pararotavirus: detection, differentiation from rotavirus, and pathogenesis in gnotobiotic pigs. J Clin Microbiol. 1982 Feb;15(2):312–319. doi: 10.1128/jcm.15.2.312-319.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bridger J. C., Clarke I. N., McCrae M. A. Characterization of an antigenically distinct porcine rotavirus. Infect Immun. 1982 Mar;35(3):1058–1062. doi: 10.1128/iai.35.3.1058-1062.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cohen J. Ribonucleic acid polymerase activity associated with purified calf rotavirus. J Gen Virol. 1977 Sep;36(3):395–402. doi: 10.1099/0022-1317-36-3-395. [DOI] [PubMed] [Google Scholar]
  5. Esparza J., Gil F. A study on the ultrastructure of human rotavirus. Virology. 1978 Nov;91(1):141–150. doi: 10.1016/0042-6822(78)90362-8. [DOI] [PubMed] [Google Scholar]
  6. Espejo R. T., López S., Arias C. Structural polypeptides of simian rotavirus SA11 and the effect of trypsin. J Virol. 1981 Jan;37(1):156–160. doi: 10.1128/jvi.37.1.156-160.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Espejo R. T., Puerto F., Soler C., González N. Characterization of a human pararotavirus. Infect Immun. 1984 Apr;44(1):112–116. doi: 10.1128/iai.44.1.112-116.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Furuichi Y. Allosteric stimulatory effect of S-adenosylmethionine on the RNA polymerase in cytoplasmic polyhedrosis virus. A model for the positive control of eukaryotic transcription. J Biol Chem. 1981 Jan 10;256(1):483–493. [PubMed] [Google Scholar]
  9. Joklik W. K. Structure and function of the reovirus genome. Microbiol Rev. 1981 Dec;45(4):483–501. doi: 10.1128/mr.45.4.483-501.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McNulty M. S., Allan G. M., Todd D., McFerran J. B., McCracken R. M. Isolation from chickens of a rotavirus lacking the rotavirus group antigen. J Gen Virol. 1981 Aug;55(Pt 2):405–413. doi: 10.1099/0022-1317-55-2-405. [DOI] [PubMed] [Google Scholar]
  11. Nicolas J. C., Cohen J., Fortier B., Lourenco M. H., Bricout F. Isolation of a human pararotavirus. Virology. 1983 Jan 15;124(1):181–184. doi: 10.1016/0042-6822(83)90302-1. [DOI] [PubMed] [Google Scholar]
  12. Rodger S. M., Bishop R. F., Holmes I. H. Detection of a rotavirus-like agent associated with diarrhea in an infant. J Clin Microbiol. 1982 Oct;16(4):724–726. doi: 10.1128/jcm.16.4.724-726.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Spencer E., Arias M. L. In vitro transcription catalyzed by heat-treated human rotavirus. J Virol. 1981 Oct;40(1):1–10. doi: 10.1128/jvi.40.1.1-10.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Spencer E., Avendaño F., Araya M. Characteristics and analysis of electropherotypes of human rotavirus isolated in Chile. J Infect Dis. 1983 Jul;148(1):41–48. doi: 10.1093/infdis/148.1.41. [DOI] [PubMed] [Google Scholar]
  15. Spencer E., García B. I. Effect of S-adenosylmethionine on human rotavirus RNA synthesis. J Virol. 1984 Oct;52(1):188–197. doi: 10.1128/jvi.52.1.188-197.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Testa D., Banerjee A. K. Initiation of RNA synthesis in vitro by vesicular stomatitis virus. Role of ATP. J Biol Chem. 1979 Mar 25;254(6):2053–2058. [PubMed] [Google Scholar]

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