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. 1986 Mar;57(3):1187–1190. doi: 10.1128/jvi.57.3.1187-1190.1986

Construction of poliovirus intertypic recombinants by use of cDNA.

G Stanway, P J Hughes, G D Westrop, D M Evans, G Dunn, P D Minor, G C Schild, J W Almond
PMCID: PMC252858  PMID: 3005620

Abstract

We investigated the use of infectious cDNA for the production of poliovirus type 1-type 3 recombinants. One such recombinant virus was produced, but a second construct involving the transfer of part of the capsid protein region was not infectious. Our results suggest that the approach may prove valuable but that not all cDNA constructs will give rise to viable viruses.

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Selected References

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  1. Agol V. I., Drozdov S. G., Frolova M. P., Grachev V. P., Kolesnikova M. S., Kozlov V. G., Ralph N. M., Romanova L. I., Tolskaya E. A., Viktorova E. G. Neurovirulence of the intertypic poliovirus recombinant v3/a1-25: characterization of strains isolated from the spinal cord of diseased monkeys and evaluation of the contribution of the 3' half of the genome. J Gen Virol. 1985 Feb;66(Pt 2):309–316. doi: 10.1099/0022-1317-66-2-309. [DOI] [PubMed] [Google Scholar]
  2. Agol V. I., Grachev V. P., Drozdov S. G., Kolesnikova M. S., Kozlov V. G., Ralph N. M., Romanova L. I., Tolskaya E. A., Tyufanov A. V., Viktorova E. G. Construction and properties of intertypic poliovirus recombinants: first approximation mapping of the major determinants of neurovirulence. Virology. 1984 Jul 15;136(1):41–55. doi: 10.1016/0042-6822(84)90246-0. [DOI] [PubMed] [Google Scholar]
  3. Cann A. J., Stanway G., Hauptmann R., Minor P. D., Schild G. C., Clarke L. D., Mountford R. C., Almond J. W. Poliovirus type 3: molecular cloning of the genome and nucleotide sequence of the region encoding the protease and polymerase proteins. Nucleic Acids Res. 1983 Mar 11;11(5):1267–1281. doi: 10.1093/nar/11.5.1267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cann A. J., Stanway G., Hughes P. J., Minor P. D., Evans D. M., Schild G. C., Almond J. W. Reversion to neurovirulence of the live-attenuated Sabin type 3 oral poliovirus vaccine. Nucleic Acids Res. 1984 Oct 25;12(20):7787–7792. doi: 10.1093/nar/12.20.7787. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Evans D. M., Dunn G., Minor P. D., Schild G. C., Cann A. J., Stanway G., Almond J. W., Currey K., Maizel J. V., Jr Increased neurovirulence associated with a single nucleotide change in a noncoding region of the Sabin type 3 poliovaccine genome. Nature. 1985 Apr 11;314(6011):548–550. doi: 10.1038/314548a0. [DOI] [PubMed] [Google Scholar]
  6. Evans D. M., Minor P. D., Schild G. S., Almond J. W. Critical role of an eight-amino acid sequence of VP1 in neutralization of poliovirus type 3. Nature. 1983 Aug 4;304(5925):459–462. doi: 10.1038/304459a0. [DOI] [PubMed] [Google Scholar]
  7. King A. M., McCahon D., Slade W. R., Newman J. W. Recombination in RNA. Cell. 1982 Jul;29(3):921–928. doi: 10.1016/0092-8674(82)90454-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kohara M., Omata T., Kameda A., Semler B. L., Itoh H., Wimmer E., Nomoto A. In vitro phenotypic markers of a poliovirus recombinant constructed from infectious cDNA clones of the neurovirulent Mahoney strain and the attenuated Sabin 1 strain. J Virol. 1985 Mar;53(3):786–792. doi: 10.1128/jvi.53.3.786-792.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Minor P. D. Characterization of strains of type 3 poliovirus by oligonucleotide mapping. J Gen Virol. 1982 Apr;59(Pt 2):307–317. doi: 10.1099/0022-1317-59-2-307. [DOI] [PubMed] [Google Scholar]
  10. Minor P. D., Evans D. M., Ferguson M., Schild G. C., Westrop G., Almond J. W. Principal and subsidiary antigenic sites of VP1 involved in the neutralization of poliovirus type 3. J Gen Virol. 1985 May;66(Pt 5):1159–1165. doi: 10.1099/0022-1317-66-5-1159. [DOI] [PubMed] [Google Scholar]
  11. Minor P. D., Schild G. C., Bootman J., Evans D. M., Ferguson M., Reeve P., Spitz M., Stanway G., Cann A. J., Hauptmann R. Location and primary structure of a major antigenic site for poliovirus neutralization. Nature. 1983 Feb 24;301(5902):674–679. doi: 10.1038/301674a0. [DOI] [PubMed] [Google Scholar]
  12. Omata T., Kohara M., Sakai Y., Kameda A., Imura N., Nomoto A. Cloned infectious complementary DNA of the poliovirus Sabin 1 genome: biochemical and biological properties of the recovered virus. Gene. 1984 Dec;32(1-2):1–10. doi: 10.1016/0378-1119(84)90026-x. [DOI] [PubMed] [Google Scholar]
  13. Parker B. A., Stark G. R. Regulation of simian virus 40 transcription: sensitive analysis of the RNA species present early in infections by virus or viral DNA. J Virol. 1979 Aug;31(2):360–369. doi: 10.1128/jvi.31.2.360-369.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Putnak J. R., Phillips B. A. Picornaviral structure and assembly. Microbiol Rev. 1981 Jun;45(2):287–315. doi: 10.1128/mr.45.2.287-315.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Racaniello V. R., Baltimore D. Cloned poliovirus complementary DNA is infectious in mammalian cells. Science. 1981 Nov 20;214(4523):916–919. doi: 10.1126/science.6272391. [DOI] [PubMed] [Google Scholar]
  16. Semler B. L., Dorner A. J., Wimmer E. Production of infectious poliovirus from cloned cDNA is dramatically increased by SV40 transcription and replication signals. Nucleic Acids Res. 1984 Jun 25;12(12):5123–5141. doi: 10.1093/nar/12.12.5123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Stanway G., Cann A. J., Hauptmann R., Hughes P., Clarke L. D., Mountford R. C., Minor P. D., Schild G. C., Almond J. W. The nucleotide sequence of poliovirus type 3 leon 12 a1b: comparison with poliovirus type 1. Nucleic Acids Res. 1983 Aug 25;11(16):5629–5643. doi: 10.1093/nar/11.16.5629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Stanway G., Hughes P. J., Mountford R. C., Minor P. D., Almond J. W. The complete nucleotide sequence of a common cold virus: human rhinovirus 14. Nucleic Acids Res. 1984 Oct 25;12(20):7859–7875. doi: 10.1093/nar/12.20.7859. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stanway G., Mountford R. C., Cox S. D., Schild G. C., Minor P. D., Almond J. W. Molecular cloning of the genomes of poliovirus type 3 strains by the cDNA: RNA hybrid method. Arch Virol. 1984;81(1-2):67–78. doi: 10.1007/BF01309297. [DOI] [PubMed] [Google Scholar]
  20. Tolskaya E. A., Romanova L. A., Kolesnikova M. S., Agol V. I. Intertypic recombination in poliovirus: genetic and biochemical studies. Virology. 1983 Jan 15;124(1):121–132. doi: 10.1016/0042-6822(83)90295-7. [DOI] [PubMed] [Google Scholar]
  21. Toyoda H., Kohara M., Kataoka Y., Suganuma T., Omata T., Imura N., Nomoto A. Complete nucleotide sequences of all three poliovirus serotype genomes. Implication for genetic relationship, gene function and antigenic determinants. J Mol Biol. 1984 Apr 25;174(4):561–585. doi: 10.1016/0022-2836(84)90084-6. [DOI] [PubMed] [Google Scholar]

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