Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1986 Jul;59(1):159–162. doi: 10.1128/jvi.59.1.159-162.1986

Direct detection of exogenous mouse mammary tumor virus sequences in lymphoid cells of BALB/cfC3H female mice.

T J Liegler, P B Blair
PMCID: PMC253051  PMID: 3012113

Abstract

The presence of exogenous mouse mammary tumor virus (MMTV) (C3H) DNA sequences in lymphoid tissue (spleen, bone marrow, and thymus) and nonlymphoid tissue (liver and kidney) of BALB/cfC3H female mice was directly assessed by DNA hybridization methods. Lymphoid tissues were found positive for integrated MMTV(C3H) sequences in females as young as 4 weeks. In most samples, the level of splenic MMTV(C3H) infection was low (2 to 5%). Infection remained throughout the life of the animal. The percentage of spleen samples found positive for exogenous viral infection was significantly higher in females bearing mammary tumors, whether virgin or multiparous. Liver and kidney DNAs were negative for exogenous MMTV sequences, suggesting tissue type selectivity in MMTV infection.

Full text

PDF
160

Images in this article

160Image
on p.160
160Image
on p.160
160Image
on p.160
161Image
on p.161

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Butel J. S., Dusing-Swartz S., Socher S. H., Medina D. Partial expression of endogenous mouse mammary tumor virus in mammary tumors induced in BALB/c mice by chemical, hormonal, and physical agents. J Virol. 1981 May;38(2):571–580. doi: 10.1128/jvi.38.2.571-580.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Calafat J., Hageman P. C. Binding of Concanavalin A to the envelope of two murine RNA tumour viruses. J Gen Virol. 1972 Jan;14(1):103–106. doi: 10.1099/0022-1317-14-1-103. [DOI] [PubMed] [Google Scholar]
  3. Cardiff R. D., Morris D. W., Young L. J. Alterations of acquired mouse mammary tumor virus DNA during mammary tumorigenesis in BALB/cfC3H mice. J Natl Cancer Inst. 1983 Nov;71(5):1011–1019. [PubMed] [Google Scholar]
  4. Charyulu V., Sigel M. M., Durden D. L., Lopez D. M. Mouse mammary tumor virus (MMTV) antigen(s) are present on B lymphocytes of BALB/c mice. Int J Cancer. 1979 Dec 15;24(6):813–818. doi: 10.1002/ijc.2910240620. [DOI] [PubMed] [Google Scholar]
  5. Cohen J. C., Majors J. E., Varmus H. E. Organization of mouse mammary tumor virus-specific DNA endogenous to BALB/c mice. J Virol. 1979 Nov;32(2):483–496. doi: 10.1128/jvi.32.2.483-496.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cohen J. C., Shank P. R., Morris V. L., Cardiff R., Varmus H. E. Integration of the DNA of mouse mammary tumor virus in virus-infected normal and neoplastic tissue of the mouse. Cell. 1979 Feb;16(2):333–345. doi: 10.1016/0092-8674(79)90010-2. [DOI] [PubMed] [Google Scholar]
  7. Dekaban G. A., Ball J. K. Integration of type B retroviral DNA in virus-induced primary murine thymic lymphomas. J Virol. 1984 Dec;52(3):784–792. doi: 10.1128/jvi.52.3.784-792.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dudley J., Risser R. Amplification and novel locations of endogenous mouse mammary tumor virus genomes in mouse T-cell lymphomas. J Virol. 1984 Jan;49(1):92–101. doi: 10.1128/jvi.49.1.92-101.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. FELDMAN D. G. Origin and distribution of virus-like particles associated with mammary tumors in DBA strain mice. II. Virus-like particles in the blood and organs. J Natl Cancer Inst. 1963 Mar;30:503–515. [PubMed] [Google Scholar]
  10. Fanning T. G., Puma J. P., Cardiff R. D. Selective amplification of mouse mammary tumor virus in mammary tumors of GR mice. J Virol. 1980 Oct;36(1):109–114. doi: 10.1128/jvi.36.1.109-114.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gillette R. W., Robertson S., Brown R., Blackman K. E. Expression of mammary tumor virus antigen on the membranes of lymphoid cells. J Natl Cancer Inst. 1974 Aug;53(2):499–505. doi: 10.1093/jnci/53.2.499. [DOI] [PubMed] [Google Scholar]
  12. HUMMEL K. P., LITTLE C. C. Comparison of the virulence of the mammary-tumor agent from four strains of mice. J Natl Cancer Inst. 1959 Oct;23:813–821. [PubMed] [Google Scholar]
  13. Hilgers J., Nowinski R. C., Geering G., Hardy W. Detection of avian and mammalian oncogenic RNA viruses (oncornaviruses) by immunofluorescence. Cancer Res. 1972 Jan;32(1):98–106. [PubMed] [Google Scholar]
  14. Hynes N. E., Groner B. Mammary tumor formation and hormonal control of mouse mammary tumor virus expression. Curr Top Microbiol Immunol. 1982;101:51–74. doi: 10.1007/978-3-642-68654-2_3. [DOI] [PubMed] [Google Scholar]
  15. Kwon B. S., Weissman S. M. Mouse mammary tumor virus-related sequences in mouse lymphocytes are inducible by 12-O-tetradecanoyl phorbol-13-acetate. J Virol. 1984 Dec;52(3):1000–1004. doi: 10.1128/jvi.52.3.1000-1004.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lopez D. M., Charyulu V., Paul R. D. B cell subsets in spleens of BALB/c mice: identification and isolation of MMTV-expressing and MMTV-responding subpopulations. J Immunol. 1985 Jan;134(1):603–607. [PubMed] [Google Scholar]
  17. Medina D., DeOme K. B., Young L. Tumor-producing capabilities of hyperplastic alveolar nodules in virgin and hormone-stimulated BALB/c f. C3H and C3Hf mice. J Natl Cancer Inst. 1970 Jan;44(1):167–174. [PubMed] [Google Scholar]
  18. Michalides R., Wagenaar E., Hilkens J., Hilgers J., Groner B., Hynes N. E. Acquisition of proviral DNA of mouse mammary tumor virus in thymic leukemia cells from GR mice. J Virol. 1982 Sep;43(3):819–829. doi: 10.1128/jvi.43.3.819-829.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Moore D. H., Long C. A., Vaidya A. B., Sheffield J. B., Dion A. S., Lasfargues E. Y. Mammary tumor viruses. Adv Cancer Res. 1979;29:347–418. doi: 10.1016/s0065-230x(08)60850-7. [DOI] [PubMed] [Google Scholar]
  20. Moore D. H., Sarkar N. H., Charney J. Bioactivity and virions in the blood of mice with mammary tumor virus. J Natl Cancer Inst. 1970 Apr;44(4):965–973. [PubMed] [Google Scholar]
  21. Nandi S., Helmich C., Haslam S. Hemic cell-associated mammary tumor virus activity in BALB-cfC3H mice. J Natl Cancer Inst. 1974 Apr;52(4):1277–1283. doi: 10.1093/jnci/52.4.1277. [DOI] [PubMed] [Google Scholar]
  22. Nusse R., Varmus H. E. Many tumors induced by the mouse mammary tumor virus contain a provirus integrated in the same region of the host genome. Cell. 1982 Nov;31(1):99–109. doi: 10.1016/0092-8674(82)90409-3. [DOI] [PubMed] [Google Scholar]
  23. Nusse R., van der Ploeg L., van Duijn L., Michalides R., Hilgers J. Impaired maturation of mouse mammary tumor virus precursor polypeptides in lymphoid leukemia cells, producing intracytoplasmic A particles and no extracellular B-type virions. J Virol. 1979 Oct;32(1):251–258. doi: 10.1128/jvi.32.1.251-258.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Osterrieth P. M., Kozma S., Hendrick J. C., François C., Calberg-Bacq C. M., Franchimont P., Gosselin L. Detection of virus antigens in Swiss albino mice infected by milk-borne mouse mammary tumour virus: the effect of age, sex and reproductive status. II. Radioimmunoassay of two virus components, gp47 and p28 in serum and organ extracts. J Gen Virol. 1979 Oct;45(1):41–50. doi: 10.1099/0022-1317-45-1-41. [DOI] [PubMed] [Google Scholar]
  25. PREHN R. T. Transfer of the mammary tumor milk agent from implant to host. J Natl Cancer Inst. 1952 Apr;12(5):1127–1139. [PubMed] [Google Scholar]
  26. Peters G., Brookes S., Smith R., Dickson C. Tumorigenesis by mouse mammary tumor virus: evidence for a common region for provirus integration in mammary tumors. Cell. 1983 Jun;33(2):369–377. doi: 10.1016/0092-8674(83)90418-x. [DOI] [PubMed] [Google Scholar]
  27. Racevskis J., Sarkar N. H. ML antigen of DBA/2 mouse leukemias: expression of an endogenous murine mammary tumor virus. J Virol. 1982 Jun;42(3):804–813. doi: 10.1128/jvi.42.3.804-813.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Ritter R. I., Nandi S. Density gradient centrifugation of red blood cells carrying the mammary tumour virus. Nature. 1968 Oct 26;220(5165):403–404. doi: 10.1038/220403a0. [DOI] [PubMed] [Google Scholar]
  29. Ritter R. I., Nandi S. Time of appearance of viral activity in red blood cells of mice infected naturally or artificially with mammary tumor virus. J Natl Cancer Inst. 1968 Jun;40(6):1313–1317. [PubMed] [Google Scholar]
  30. Shank P. R., Cohen J. C., Varmus H. E., Yamamoto K. R., Ringold G. M. Mapping of linear and circular forms of mouse mammary tumor virus DNA with restriction endonucleases: evidence for a large specific deletion occurring at high frequency during circularization. Proc Natl Acad Sci U S A. 1978 May;75(5):2112–2116. doi: 10.1073/pnas.75.5.2112. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Smith G. H. Role of the milk agent in disappearance of mammary cancer in C3H/StWi mice. J Natl Cancer Inst. 1966 Apr;36(4):685–701. doi: 10.1093/jnci/36.4.685. [DOI] [PubMed] [Google Scholar]
  32. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  33. Tax A., Ewert D., Manson L. A. An antigen cross-reactive with gp52 of mammary tumor virus is expressed on a B cell subpopulation of mice. J Immunol. 1983 May;130(5):2368–2371. [PubMed] [Google Scholar]
  34. Vaidya A. B., Long C. A., Sheffield J. B., Tamura A., Tanaka H. Murine mammary tumor virus deficient in the major glycoprotein: biochemical and biological studies on virions produced by a lymphoma cell line. Virology. 1980 Jul 30;104(2):279–293. doi: 10.1016/0042-6822(80)90333-5. [DOI] [PubMed] [Google Scholar]
  35. Wheeler D. A., Butel J. S., Medina D., Cardiff R. D., Hager G. L. Transcription of mouse mammary tumor virus: identification of a candidate mRNA for the long terminal repeat gene product. J Virol. 1983 Apr;46(1):42–49. doi: 10.1128/jvi.46.1.42-49.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES