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. 1988 May;62(5):1819–1823. doi: 10.1128/jvi.62.5.1819-1823.1988

Prediction of human rotavirus serotype by nucleotide sequence analysis of the VP7 protein gene.

K Y Green 1, J F Sears 1, K Taniguchi 1, K Midthun 1, Y Hoshino 1, M Gorziglia 1, K Nishikawa 1, S Urasawa 1, A Z Kapikian 1, R M Chanock 1, et al.
PMCID: PMC253238  PMID: 2833626

Abstract

Human rotavirus field isolates were characterized by direct sequence analysis of the gene encoding the serotype-specific major neutralization protein (VP7). Single-stranded RNA transcripts were prepared from virus particles obtained directly from stool specimens or after two or three passages in MA-104 cells. Two regions of the gene (nucleotides 307 through 351 and 670 through 711) which had previously been shown to contain regions of sequence divergence among rotavirus serotypes were sequenced by the dideoxynucleotide method with two different synthetic oligonucleotide primers. The resulting nucleotide sequences were compared with the corresponding sequences from rotaviruses of known serotype (serotype 1, 2, 3, or 4). A total of 25 field isolates and 10 laboratory strains examined by this method exhibited marked sequence identity in both areas of the gene with the corresponding regions of 1 of the 4 reference strains. In addition, the predicted serotype from the sequence analysis correlated in each case with the serotype determined when the rotaviruses were examined by plaque reduction neutralization or reactivity with serotype-specific monoclonal antibodies. These data suggest that as a result of the high degree of sequence conservation observed among rotaviruses of the same serotype, it is possible to predict the serotype of a rotavirus isolate by direct sequence analysis of its VP7 gene.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albert M. J., Unicomb L. E., Bishop R. F. Cultivation and characterization of human rotaviruses with "super short" RNA patterns. J Clin Microbiol. 1987 Jan;25(1):183–185. doi: 10.1128/jcm.25.1.183-185.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bastardo J. W., McKimm-Breschkin J. L., Sonza S., Mercer L. D., Holmes I. H. Preparation and characterization of antisera to electrophoretically purified SA11 virus polypeptides. Infect Immun. 1981 Dec;34(3):641–647. doi: 10.1128/iai.34.3.641-647.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Beards G. M., Pilfold J. N., Thouless M. E., Flewett T. H. Rotavirus serotypes by serum neutralisation. J Med Virol. 1980;5(3):231–237. doi: 10.1002/jmv.1890050307. [DOI] [PubMed] [Google Scholar]
  4. Beck E., Strohmaier K. Subtyping of European foot-and-mouth disease virus strains by nucleotide sequence determination. J Virol. 1987 May;61(5):1621–1629. doi: 10.1128/jvi.61.5.1621-1629.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Clark H. F., Hoshino Y., Bell L. M., Groff J., Hess G., Bachman P., Offit P. A. Rotavirus isolate WI61 representing a presumptive new human serotype. J Clin Microbiol. 1987 Sep;25(9):1757–1762. doi: 10.1128/jcm.25.9.1757-1762.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Coulson B. S., Unicomb L. E., Pitson G. A., Bishop R. F. Simple and specific enzyme immunoassay using monoclonal antibodies for serotyping human rotaviruses. J Clin Microbiol. 1987 Mar;25(3):509–515. doi: 10.1128/jcm.25.3.509-515.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Coulson B. S. Variation in neutralization epitopes of human rotaviruses in relation to genomic RNA polymorphism. Virology. 1987 Aug;159(2):209–216. doi: 10.1016/0042-6822(87)90457-0. [DOI] [PubMed] [Google Scholar]
  8. Dyall-Smith M. L., Lazdins I., Tregear G. W., Holmes I. H. Location of the major antigenic sites involved in rotavirus serotype-specific neutralization. Proc Natl Acad Sci U S A. 1986 May;83(10):3465–3468. doi: 10.1073/pnas.83.10.3465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Flores J., Myslinski J., Kalica A. R., Greenberg H. B., Wyatt R. G., Kapikian A. Z., Chanock R. M. In vitro transcription of two human rotaviruses. J Virol. 1982 Sep;43(3):1032–1037. doi: 10.1128/jvi.43.3.1032-1037.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Flores J., Perez-Schael I., Gonzalez M., Garcia D., Perez M., Daoud N., Cunto W., Chanock R. M., Kapikian A. Z. Protection against severe rotavirus diarrhoea by rhesus rotavirus vaccine in Venezuelan infants. Lancet. 1987 Apr 18;1(8538):882–884. doi: 10.1016/s0140-6736(87)92858-3. [DOI] [PubMed] [Google Scholar]
  11. Glass R. I., Keith J., Nakagomi O., Nakagomi T., Askaa J., Kapikian A. Z., Chanock R. M., Flores J. Nucleotide sequence of the structural glycoprotein VP7 gene of Nebraska calf diarrhea virus rotavirus: comparison with homologous genes from four strains of human and animal rotaviruses. Virology. 1985 Mar;141(2):292–298. doi: 10.1016/0042-6822(85)90260-0. [DOI] [PubMed] [Google Scholar]
  12. Green K. Y., Midthun K., Gorziglia M., Hoshino Y., Kapikian A. Z., Chanock R. M., Flores J. Comparison of the amino acid sequences of the major neutralization protein of four human rotavirus serotypes. Virology. 1987 Nov;161(1):153–159. doi: 10.1016/0042-6822(87)90181-4. [DOI] [PubMed] [Google Scholar]
  13. Greenberg H. B., Flores J., Kalica A. R., Wyatt R. G., Jones R. Gene coding assignments for growth restriction, neutralization and subgroup specificities of the W and DS-1 strains of human rotavirus. J Gen Virol. 1983 Feb;64(Pt 2):313–320. doi: 10.1099/0022-1317-64-2-313. [DOI] [PubMed] [Google Scholar]
  14. Gunn P. R., Sato F., Powell K. F., Bellamy A. R., Napier J. R., Harding D. R., Hancock W. S., Siegman L. J., Both G. W. Rotavirus neutralizing protein VP7: antigenic determinants investigated by sequence analysis and peptide synthesis. J Virol. 1985 Jun;54(3):791–797. doi: 10.1128/jvi.54.3.791-797.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Heath R., Birch C., Gust I. Antigenic analysis of rotavirus isolates using monoclonal antibodies specific for human serotypes 1, 2, 3 and 4, and SA11. J Gen Virol. 1986 Nov;67(Pt 11):2455–2466. doi: 10.1099/0022-1317-67-11-2455. [DOI] [PubMed] [Google Scholar]
  16. Hoshino Y., Wyatt R. G., Greenberg H. B., Flores J., Kapikian A. Z. Serotypic similarity and diversity of rotaviruses of mammalian and avian origin as studied by plaque-reduction neutralization. J Infect Dis. 1984 May;149(5):694–702. doi: 10.1093/infdis/149.5.694. [DOI] [PubMed] [Google Scholar]
  17. Kalica A. R., Greenberg H. B., Wyatt R. G., Flores J., Sereno M. M., Kapikian A. Z., Chanock R. M. Genes of human (strain Wa) and bovine (strain UK) rotaviruses that code for neutralization and subgroup antigens. Virology. 1981 Jul 30;112(2):385–390. doi: 10.1016/0042-6822(81)90285-3. [DOI] [PubMed] [Google Scholar]
  18. Matsuno S., Hasegawa A., Mukoyama A., Inouye S. A candidate for a new serotype of human rotavirus. J Virol. 1985 May;54(2):623–624. doi: 10.1128/jvi.54.2.623-624.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Midthun K., Flores J., Taniguchi K., Urasawa S., Kapikian A. Z., Chanock R. M. Genetic relatedness among human rotavirus genes coding for VP7, a major neutralization protein, and its application to serotype identification. J Clin Microbiol. 1987 Jul;25(7):1269–1274. doi: 10.1128/jcm.25.7.1269-1274.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rico-Hesse R., Pallansch M. A., Nottay B. K., Kew O. M. Geographic distribution of wild poliovirus type 1 genotypes. Virology. 1987 Oct;160(2):311–322. doi: 10.1016/0042-6822(87)90001-8. [DOI] [PubMed] [Google Scholar]
  21. Shaw R. D., Stoner-Ma D. L., Estes M. K., Greenberg H. B. Specific enzyme-linked immunoassay for rotavirus serotypes 1 and 3. J Clin Microbiol. 1985 Aug;22(2):286–291. doi: 10.1128/jcm.22.2.286-291.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Taniguchi K., Urasawa T., Morita Y., Greenberg H. B., Urasawa S. Direct serotyping of human rotavirus in stools by an enzyme-linked immunosorbent assay using serotype 1-, 2-, 3-, and 4-specific monoclonal antibodies to VP7. J Infect Dis. 1987 Jun;155(6):1159–1166. doi: 10.1093/infdis/155.6.1159. [DOI] [PubMed] [Google Scholar]
  23. Thouless M. E., Bryden A. S., Flewett T. H. Serotypes of human rotavirus. Lancet. 1978 Jan 7;1(8054):39–39. doi: 10.1016/s0140-6736(78)90381-1. [DOI] [PubMed] [Google Scholar]
  24. Wyatt R. G., Greenberg H. B., James W. D., Pittman A. L., Kalica A. R., Flores J., Chanock R. M., Kapikian A. Z. Definition of human rotavirus serotypes by plaque reduction assay. Infect Immun. 1982 Jul;37(1):110–115. doi: 10.1128/iai.37.1.110-115.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wyatt R. G., James H. D., Jr, Pittman A. L., Hoshino Y., Greenberg H. B., Kalica A. R., Flores J., Kapikian A. Z. Direct isolation in cell culture of human rotaviruses and their characterization into four serotypes. J Clin Microbiol. 1983 Aug;18(2):310–317. doi: 10.1128/jcm.18.2.310-317.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]

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