Skip to main content
Bulletin of the World Health Organization logoLink to Bulletin of the World Health Organization
. 1983;61(2):307–316.

Enzyme analysis of Schistosoma haematobium*

C A Wright, G C Ross
PMCID: PMC2536131  PMID: 6222843

Abstract

Results are reported of enzyme analyses, by isoelectric focusing in polyacrylamide gels, of adult Schistosoma haematobium worms derived from 22 isolates originating from 13 countries. Polymorphisms have been identified in the glucose-6-phosphate dehydrogenase (G6PD) and phosphoglucomutase (PGM) systems. Certain forms appear to be restricted in their geographical distribution and their occurrence outside their usual areas suggests human population movements resulting in mixing of parasite strains. The possible implications of minor variations in some PGM patterns and the apparent absence of heteropolymer fractions in presumed G6PD heterozygotes are discussed. The use of the technique to detect natural multiple miracidial infections in snails is reported and discussed.

Full text

PDF
309

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Coles G. C. A comparison of some isoenzymes of Schistosoma mansoni and Schistosoma haematobium. Comp Biochem Physiol. 1970 Apr 1;33(3):549–558. doi: 10.1016/0010-406x(70)90370-1. [DOI] [PubMed] [Google Scholar]
  2. Conde-del Pino E., Annexy-Martínez A. M., Pérez-Vilar M., Cintrón-Rivera A. A. Studies in Schistosoma mansoni. II. Isoenzyme patterns for alkaline phosphatase, isocitric dehydrogenase, glutamic oxalacetic transaminase, and glucose 6-phosphate dehydrogenase of adult worms and cercariae. Exp Parasitol. 1968 Jun;22(3):288–294. doi: 10.1016/0014-4894(68)90104-5. [DOI] [PubMed] [Google Scholar]
  3. Fletcher M., LoVerde P. T., Woodruff D. S. Genetic variation in Schistosoma mansoni: enzyme polymorphisms in populations from Africa, Southwest Asia, South America, and the West Indies. Am J Trop Med Hyg. 1981 Mar;30(2):406–421. doi: 10.4269/ajtmh.1981.30.406. [DOI] [PubMed] [Google Scholar]
  4. Jourdane J., Theron A. Schistosoma mansoni: cloning by microsurgical transplantation of sporocysts. Exp Parasitol. 1980 Dec;50(3):349–357. doi: 10.1016/0014-4894(80)90038-7. [DOI] [PubMed] [Google Scholar]
  5. Ross G. C., Southgate V. R., Knowles R. J. Observations on some isoenzymes of strains of Schistosoma bovis; S. mattheei, S. margrebowiei, and S. leiperi. Z Parasitenkd. 1978 Sep 4;57(1):49–56. doi: 10.1007/BF00927628. [DOI] [PubMed] [Google Scholar]
  6. Ruff M. D., Davis G. M., Werner J. K. Schistosoma japonicum: disc electrophoretic protein patterns of the Japanese, Philippine, and Formosan strains. Exp Parasitol. 1973 Jun;33(3):437–446. doi: 10.1016/0014-4894(73)90111-2. [DOI] [PubMed] [Google Scholar]
  7. Southgate V. R., Rollinson D., Ross G. C., Knowles R. J. Observations on an isolate of Schistosoma bovis from Tanzania. Z Parasitenkd. 1980;63(3):241–249. doi: 10.1007/BF00931986. [DOI] [PubMed] [Google Scholar]
  8. Wright C. A., Bennett M. S. Studies on Schistosoma haematobium in the laboratory. I. A strain from Durban, Natal, South Africa. Trans R Soc Trop Med Hyg. 1967;61(2):221–227. doi: 10.1016/0035-9203(67)90160-5. [DOI] [PubMed] [Google Scholar]
  9. Wright C. A., Bennett M. S. Studies on Schistosoma haematobium in the laboratory. II. A strain from South Arabia. Trans R Soc Trop Med Hyg. 1967;61(2):228–233. doi: 10.1016/0035-9203(67)90161-7. [DOI] [PubMed] [Google Scholar]
  10. Wright C. A., Knowles R. J. Studies on Schistosoma haematobium in the laboratory. 3. Strains from Iran, Mauritius and Ghana. Trans R Soc Trop Med Hyg. 1972;66(1):108–118. doi: 10.1016/0035-9203(72)90057-0. [DOI] [PubMed] [Google Scholar]
  11. Wright C. A., Ross G. C. Hybrids between Schistosoma haematobium and S. mattheei and their identification by isoelectric focusing of enzymes. Trans R Soc Trop Med Hyg. 1980;74(3):326–332. doi: 10.1016/0035-9203(80)90091-7. [DOI] [PubMed] [Google Scholar]
  12. Wright C. A., Southgate V. R., Ross G. C. Enzymes in Schistosoma intercalatum and the relative status of the Lower Guinea and Zaire strains of the parasite. Int J Parasitol. 1979 Dec;9(6):523–528. doi: 10.1016/0020-7519(79)90007-9. [DOI] [PubMed] [Google Scholar]
  13. Yan S. L., Hsu S. H., Cross J. H. Malic dehydrogenase patterns in different strains of Schistosoma japonicum and subspecies of Oncomelania hupensis. Zhonghua Min Guo Wei Sheng Wu Xue Za Zhi. 1976 Dec;9(3-4):83–86. [PubMed] [Google Scholar]

Articles from Bulletin of the World Health Organization are provided here courtesy of World Health Organization

RESOURCES