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. 1988 Nov;62(11):4086–4095. doi: 10.1128/jvi.62.11.4086-4095.1988

Negative regulation of retrovirus expression in embryonal carcinoma cells mediated by an intragenic domain.

T P Loh 1, L L Sievert 1, R W Scott 1
PMCID: PMC253839  PMID: 3172339

Abstract

An intragenic region spanning the tRNA primer binding site of a Moloney murine leukemia virus recombinant retrovirus was found to restrict expression specifically in embryonal carcinoma (EC) cells. When the inhibitory domain was present, the levels of steady-state RNA synthesized from integrated recombinant templates in stable cotransformation assays were reduced 20-fold in EC cells but not in C2 myoblast cells. Transient-cotransfection assays showed that repression of a template containing the EC-specific inhibitory component was relieved by an excess of specific competitor DNA. In addition, repression mediated by the inhibitory component was orientation independent. This evidence demonstrates the presence of a saturable, trans-acting negative regulatory factor(s) in EC cells and suggests that the interaction of the factor(s) with the intragenic inhibitory component occurs at the DNA level.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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