Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1988 Nov;62(11):4362–4364. doi: 10.1128/jvi.62.11.4362-4364.1988

Evaluation of the interaction of phi X174 gene products E and K in E-mediated lysis of Escherichia coli.

U Bläsi 1, R Young 1, W Lubitz 1
PMCID: PMC253874  PMID: 2971822

Abstract

Gene K of bacteriophage phi X174 was cloned, and its gene product was localized in the cell envelope of Escherichia coli. Compared with the sole expression of the phi X174 lysis gene E, the simultaneous expression of the K and E genes had no effect on scheduling of cell lysis. Therefore, a direct interaction of proteins E and K could be excluded. In contrast, phi X174 infection of a host carrying a plasmid expressing gene K resulted in a delayed lysis and an apparent increase in phage titer.

Full text

PDF
4364

Images in this article

4363Image
on p.4363

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Altman E., Young K., Garrett J., Altman R., Young R. Subcellular localization of lethal lysis proteins of bacteriophages lambda and phiX174. J Virol. 1985 Mar;53(3):1008–1011. doi: 10.1128/jvi.53.3.1008-1011.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barrell B. G., Air G. M., Hutchison C. A., 3rd Overlapping genes in bacteriophage phiX174. Nature. 1976 Nov 4;264(5581):34–41. doi: 10.1038/264034a0. [DOI] [PubMed] [Google Scholar]
  3. Bläsi U., Henrich B., Lubitz W. Lysis of Escherichia coli by cloned phi X174 gene E depends on its expression. J Gen Microbiol. 1985 May;131(5):1107–1114. doi: 10.1099/00221287-131-5-1107. [DOI] [PubMed] [Google Scholar]
  4. Gillam S., Atkinson T., Markham A., Smith M. Gene K of bacteriophage phi X174 codes for a protein which affects the burst size of phage production. J Virol. 1985 Feb;53(2):708–709. doi: 10.1128/jvi.53.2.708-709.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Henrich B., Lubitz W., Plapp R. Lysis of Escherichia coli by induction of cloned phi X174 genes. Mol Gen Genet. 1982;185(3):493–497. doi: 10.1007/BF00334146. [DOI] [PubMed] [Google Scholar]
  6. Hutchison C. A., 3rd, Sinsheimer R. L. The process of infection with bacteriophage phi-X174. X. Mutations in a phi-X Lysis gene. J Mol Biol. 1966 Jul;18(3):429–447. doi: 10.1016/s0022-2836(66)80035-9. [DOI] [PubMed] [Google Scholar]
  7. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  8. Remaut E., Stanssens P., Fiers W. Plasmid vectors for high-efficiency expression controlled by the PL promoter of coliphage lambda. Gene. 1981 Oct;15(1):81–93. doi: 10.1016/0378-1119(81)90106-2. [DOI] [PubMed] [Google Scholar]
  9. Sanger F., Coulson A. R., Friedmann T., Air G. M., Barrell B. G., Brown N. L., Fiddes J. C., Hutchison C. A., 3rd, Slocombe P. M., Smith M. The nucleotide sequence of bacteriophage phiX174. J Mol Biol. 1978 Oct 25;125(2):225–246. doi: 10.1016/0022-2836(78)90346-7. [DOI] [PubMed] [Google Scholar]
  10. Shaw D. C., Walker J. E., Northrop F. D., Barrell B. G., Godson G. N., Fiddes J. C. Gene K, a new overlapping gene in bacteriophage G4. Nature. 1978 Apr 6;272(5653):510–515. doi: 10.1038/272510a0. [DOI] [PubMed] [Google Scholar]
  11. Sinsheimer R. L. Bacteriophage phi-X174 and related viruses. Prog Nucleic Acid Res Mol Biol. 1968;8:115–169. [PubMed] [Google Scholar]
  12. Tessman E. S., Tessman I., Pollock T. J. Gene K of bacteriophage phi X 174 codes for a nonessential protein. J Virol. 1980 Jan;33(1):557–560. doi: 10.1128/jvi.33.1.557-560.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Young K. D., Young R. Lytic action of cloned phi X174 gene E. J Virol. 1982 Dec;44(3):993–1002. doi: 10.1128/jvi.44.3.993-1002.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES